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DNA Labs India

Human Immunodeficiency Virus 1 & 2 Antibodies Western Blot Test

DNA Labs India | ISO 9001:2015 Certified

Human Immunodeficiency Virus 1 & 2 Antibodies Western Blot Test

Short Name: HIV 1&2 Western Blot Test

Also known as: HIV Western Blot Test, HIV Antibody Confirmation Test, HIV 1/2 Western Blot

Human Immunodeficiency Virus 1 & 2 Antibodies Western Blot Test test available at DNA Labs India for ₹4,800. Uses Western Blot on Serum samples. Results in Same day for report after sample collection on Tue/Thu/Sat by 9 am. Free home collection in 300+ cities across India.

Confirmatory🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Kothari Ramesh Shantilal

Dr Kothari Ramesh Shantilal

Consultant Microbiologist · Reg: 27670

Last reviewed: September 7, 2026

Overview

To confirm the presence of HIV-1 and HIV-2 antibodies after initial screening, providing a definitive diagnosis for appropriate medical management.

Test Code
769
Price
₹4,800
Sample Type
Serum
Result Time
Same day for report after sample collection on Tue/Thu/Sat by 9 am
Fasting Required
No
Method
Western Blot
Step 1

Sample Collection

Duly filled Consent form for HIV testing & Pre-test counselling (Form 10 & 10A) is mandatory. No fasting required.

Method: Blood Draw

Step 2

Laboratory Analysis

Standard blood draw from vein, typically in the arm.

Step 3

Report Delivery

Apply pressure to the site to stop bleeding; mild soreness may occur.

Timeline: Same day for report after sample collection on Tue/Thu/Sat by 9 am

Patient Instructions

1
Before the Test:Complete consent and counselling forms; no special preparation needed.
2
During the Test:Simple blood draw; minimal discomfort.
3
After the Test:Resume normal activities; monitor for any adverse effects at puncture site.

About This Test

Who Should Get This Test

To confirm the presence of HIV-1 and HIV-2 antibodies after initial screening, providing a definitive diagnosis for appropriate medical management.

How to Prepare

  • Complete mandatory consent and counselling forms
  • Use primary sample tube only; aliquoted samples not accepted
  • Ship samples refrigerated or frozen

Doctor's Notes

Reviewed by — MBBS, MD (Microbiology) · Reg. No. 27670

"The HIV Western Blot test is essential for confirming HIV diagnosis after initial screening, ensuring high accuracy and guiding appropriate treatment."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL (1 mL min.)
ContainerSST (Serum Separator Tube)
Collection MethodBlood Draw

Sample Stability

Room Temperature: 8 hours
Refrigerator: 1 week
Frozen: 4 weeks
Sample Rejection Criteria:
  • Aliquoted samples not accepted
  • Only primary sample tube accepted
  • Incomplete consent forms

Understanding Your Results

Results are reported as reactive, non-reactive, or indeterminate based on antibody detection.
📊

Reactive

Indicates presence of HIV antibodies; confirm with clinical correlation.

📊

Non-reactive

No HIV antibodies detected; but may not rule out very early infection.

📊

Indeterminate

Inconclusive; further testing or clinical evaluation recommended.

⚠️ When to Consult a Doctor:

Consult a doctor if you have a positive or indeterminate result, persistent symptoms, or known exposure to HIV.

Limitations

  • ⚠Indeterminate results may occur in early infections or certain conditions
  • ⚠Not used as a standalone screening test
  • ⚠Requires confirmatory interpretation by healthcare provider

Risks & Considerations

  • ●Minor bruising or pain at blood draw site
  • ●Very rare risk of infection or excessive bleeding

Interfering Factors

  • ●Early HIV 1/2 infections
  • ●Autoimmune diseases
  • ●Pregnancy
  • ●Recent Tetanus Toxoid administration

Compare With Similar Tests

TestHuman Immunodeficiency Virus 1 & 2 Antibodies Western Blot TestELISARapid HIV TestHIV RNA PCR
ComparisonHuman Immunodeficiency Virus 1 & 2 Antibodies Western Blot Test

Frequently Asked Questions

What is the HIV Western Blot test?
It is a confirmatory blood test that detects antibodies to specific HIV proteins, used after a positive screening test like ELISA.
When should I get the HIV Western Blot test?
You should get it if you have a positive initial HIV screening test, symptoms of HIV, or potential exposure to the virus.
How is the test performed?
A blood sample is collected and analyzed in the lab using the Western Blot method to detect HIV-1 and HIV-2 antibodies.
What does a positive result mean?
A positive result indicates the presence of HIV antibodies, suggesting infection. Consult a doctor for confirmation and next steps.
What does a negative result mean?
A negative result means no HIV antibodies were detected, but it may not rule out very early infection. Discuss with your doctor if exposure was recent.
Are there any risks associated with this test?
Risks are minimal, such as minor bruising from the blood draw. There are no significant health risks from the test itself.
How accurate is the HIV Western Blot test?
When combined with ELISA, specificity exceeds 99.9%, making it highly accurate for confirming HIV diagnosis.
What is the cost of the test?
The cost is INR 4800 at DNA Labs India, with potential discounts or home collection options.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection for this test across many cities in India.
How long does it take to get results?
Results are typically available same day after sample collection on Tuesday, Thursday, or Saturday by 9 am.
What should I do after the test?
Follow up with your healthcare provider to discuss results and any necessary treatment or monitoring.
Is this test covered by insurance?
Coverage varies by insurance plan; check with your provider. It may not be covered under government schemes like PMJAY or CGHS.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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