Human Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 CD4 / CD8 (Immune Deficiency Panel 2) Markers Test
Short Name: HIV-1 RNA PCR with CD3/CD4/CD8 Test
Also known as: HIV Viral Load Test with Immune Markers, Immune Deficiency Panel 2
Human Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 CD4 / CD8 (Immune Deficiency Panel 2) Markers Test test available at DNA Labs India for ₹8,190. Uses Real Time RT-PCR, Flow Cytometry on PCR: Plasma from EDTA tubes; Flow: Whole blood from EDTA and Sodium Heparin tubes samples. Results in Sample collection on Wed/Sat by 11 am; reports available on Fri/Tue.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
This panel is used to assess patient prognosis and monitor the effect of anti-retroviral therapy in HIV-1 infected patients. It helps in evaluating immune deficiency status and guiding clinical management.
- Test Code
- 771
- Price
- ₹8,190
- Sample Type
- PCR: Plasma from EDTA tubes; Flow: Whole blood from EDTA and Sodium Heparin tubes
- Result Time
- Sample collection on Wed/Sat by 11 am; reports available on Fri/Tue.
- Fasting Required
- Yes (12 hours)
- Method
- Real Time RT-PCR, Flow Cytometry
Sample Collection
12 hours fasting is recommended. Inform your physician about any medications or health conditions.
Method: Venipuncture
Laboratory Analysis
A blood sample will be drawn from a vein in your arm by a trained professional.
Report Delivery
Apply pressure to the puncture site to stop bleeding. Avoid heavy lifting for a few hours.
Timeline: Sample collection on Wed/Sat by 11 am; reports available on Fri/Tue.
Patient Instructions
About This Test
Who Should Get This Test
This panel is used to assess patient prognosis and monitor the effect of anti-retroviral therapy in HIV-1 infected patients. It helps in evaluating immune deficiency status and guiding clinical management.
How to Prepare
- PCR: Collect 3 mL plasma from 2 Lavender Top (EDTA) tubes; separate plasma aseptically within 2 hours of collection
- Flow: Collect 4 mL whole blood from 1 Lavender Top (EDTA) tube and 1 Green Top (Sodium Heparin) tube
- Ship PCR samples refrigerated or frozen, and Flow samples at 18-22°C; do not refrigerate or freeze Flow samples
- Specify time, date, and clinical details on the Test Request form
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"This test is essential for managing HIV-1 patients to monitor viral load and immune status, helping guide antiretroviral therapy and assess prognosis."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Incorrect sample type or volume
- Hemolyzed or lipemic samples
- Improper labeling or documentation
Understanding Your Results
High HIV-1 RNA
Indicates active viral replication and may require therapy adjustment
Low or undetectable HIV-1 RNA
Suggests effective antiretroviral therapy control
Low CD4 count
Indicates immune suppression and higher risk of opportunistic infections
Altered CD4/CD8 ratio
May reflect immune dysregulation in HIV infection
Consult your doctor if you experience symptoms like fever, weight loss, or recurrent infections, or if test results show abnormal viral load or immune markers. Regular monitoring is advised for HIV patients.
Limitations
- ⚠This test is not for initial HIV diagnosis; antibody tests are preferred
- ⚠Results may vary based on sample handling and timing
- ⚠Interpretation requires clinical context and physician guidance
Risks & Considerations
- ●Minor bruising or pain at the blood draw site
- ●Rare risk of infection or dizziness
Interfering Factors
- ●Anticoagulant use may affect sample integrity
- ●Contaminated samples can lead to inaccurate results
- ●Recent vaccinations or infections might alter immune markers
Compare With Similar Tests
| Test | Human Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 CD4 / CD8 (Immune Deficiency Panel 2) Markers Test | HIV Antibody Test | CD4 Count Test | HIV-1 RNA Qualitative PCR |
|---|---|---|---|---|
| Comparison | Human Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 CD4 / CD8 (Immune Deficiency Panel 2) Markers Test |
Frequently Asked Questions
What is the HIV-1 RNA Quantitative Real Time PCR with CD3 CD4 / CD8 test?
Why is this test performed?
How is the test different from an HIV antibody test?
What do CD3, CD4, and CD8 markers indicate?
Is fasting required for this test?
How long does it take to get results?
What sample is needed for this test?
Can this test be used to diagnose HIV?
What does a high HIV-1 RNA level mean?
How often should this test be done?
Is home sample collection available?
What should I do if my results are abnormal?
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