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DNA Labs India

HLA T & B Cell Lymphocyte Crossmatch Test

DNA Labs India | ISO 9001:2015 Certified

HLA T & B Cell Lymphocyte Crossmatch Test

Short Name: HLA T & B Cell Crossmatch

Also known as: HLA Crossmatch, T & B Cell Crossmatch, Lymphocyte Crossmatch, CDC Crossmatch

HLA T & B Cell Lymphocyte Crossmatch Test test available at DNA Labs India for ₹10,000. Uses Complement Dependent Cytotoxicity with DTT treatment on Whole blood and serum samples. Results in Reports are typically available within 10-12 days from sample receipt.. Free home collection in 300+ cities across India.

NephrologistAdults and children as per transplant protocol🏠 Home Collection

✍️ Written By

Overview

To assess immunological compatibility between donor and recipient prior to transplantation or blood transfusion by detecting preformed antibodies against donor T and B lymphocytes.

Test Code
3483
Price
₹10,000
Sample Type
Whole blood and serum
Result Time
Reports are typically available within 10-12 days from sample receipt.
Fasting Required
No
Method
Complement Dependent Cytotoxicity with DTT treatment
Step 1

Sample Collection

Recipient sample should be collected preferably 48 hours after dialysis. No fasting is required. Bring doctor's prescription on letterhead with stamp, 1 photograph each of recipient and donor, and completed Solid Organ Transplant Consent form (Form 33) when applicable.

Method: Venipuncture

Step 2

Laboratory Analysis

A healthcare professional will draw blood by venipuncture. Recipient sample includes whole blood in ACD tube and serum in SST. Donor sample includes whole blood in ACD tubes.

Step 3

Report Delivery

Samples must be shipped refrigerated and should not be frozen. Reports are usually delivered within 10-12 days via online portal, email, or WhatsApp.

Timeline: Reports are typically available within 10-12 days from sample receipt.

Patient Instructions

1
Before the Test:No fasting is required. Bring mandatory documents and collect recipient sample preferably 48 hours after dialysis.
2
During the Test:Venipuncture is performed to collect donor and recipient blood samples.
3
After the Test:Samples are shipped refrigerated. Avoid freezing. Reports take approximately 10-12 days.

About This Test

Who Should Get This Test

To assess immunological compatibility between donor and recipient prior to transplantation or blood transfusion by detecting preformed antibodies against donor T and B lymphocytes.

How to Prepare

  • Collect recipient sample preferably 48 hours after dialysis.
  • Recipient: Collect 6 mL whole blood in 1 Yellow Top ACD tube and 3 mL serum in 1 SST.
  • Donor: Collect 12 mL whole blood in 2 Yellow Top ACD tubes.
  • Ship refrigerated; do not freeze.
  • Ensure mandatory doctor's prescription with stamp, 1 photograph each of recipient and donor, and Solid Organ Transplant Consent form Form 33 are provided.

Doctor's Notes

"For pre-transplant patients, a negative HLA T & B cell crossmatch is a key requirement before proceeding. Always collect samples as per protocol and provide mandatory consent, prescription, and photographs."

Test Parameters & Specifications

Sample TypeWhole blood and serum
Sample VolumeRecipient: 6 mL whole blood + 3 mL serum; Donor: 12 mL whole blood
ContainerRecipient: 1 Yellow Top (ACD) tube and 1 SST; Donor: 2 Yellow Top (ACD) tubes
Collection MethodVenipuncture

Sample Stability

Room TemperatureNot applicable
Refrigerated 2-8 degree Celsius48 hours
FrozenNot applicable; do not freeze
Sample Rejection Criteria:
  • Frozen sample
  • Inadequate sample volume
  • Missing mandatory prescription, consent form, or photographs
  • Improperly labeled specimen

Understanding Your Results

The crossmatch is reported as positive or negative. A negative result suggests no detectable donor-specific antibodies in the tested conditions. A positive result indicates antibody reactivity and requires correlation with clinical history and additional donor-specific antibody testing.
Negative T cell crossmatch: no significant T-cell reactive antibodies detected.
Positive T cell crossmatch: suggests high risk of antibody-mediated rejection; transplant may be contraindicated or require desensitization.
Negative B cell crossmatch: no significant B-cell reactive antibodies detected.
Positive B cell crossmatch: may indicate HLA class I or II antibodies and should be evaluated with donor-specific antibody testing.
⚠️ When to Consult a Doctor:

If the result is positive or if there is a history of prior transplant, blood transfusion, or pregnancy, discuss the report with a transplant nephrologist or immunologist.

Limitations

  • ⚠CDC crossmatch may not detect very low levels of donor-specific antibodies
  • ⚠B cell crossmatch may be positive due to non-HLA antibodies
  • ⚠Does not replace DSA testing or clinical assessment
  • ⚠Requires live donor lymphocytes; sample viability affects results

Risks & Considerations

  • ●Mild pain or bruising at venipuncture site
  • ●Slight bleeding or hematoma
  • ●Light-headedness during blood draw

Interfering Factors

  • ●Frozen or improperly stored specimens
  • ●Incomplete clinical history or consent documentation
  • ●Recent dialysis timing deviations
  • ●Improper sample labeling
  • ●Drugs affecting immune response may influence interpretation

Compare With Similar Tests

TestHLA T & B Cell Lymphocyte Crossmatch Test
ComparisonHLA T & B Cell Lymphocyte Crossmatch TestDetects HLA antibodies in serum; crossmatch directly tests reaction against donor cells.More sensitive than CDC crossmatch and may detect low-level donor-specific antibodies.Identifies specific HLA antibody targets; crossmatch shows biological reactivity.

Frequently Asked Questions

What is HLA T & B Cell Lymphocyte Crossmatch test?
It is a laboratory test that checks whether a recipient has preformed antibodies that may react against donor T and B lymphocytes before organ transplantation or blood transfusion.
Why is this test required before transplant?
It helps assess donor-recipient compatibility and reduces the risk of antibody-mediated rejection or adverse reaction after transplantation or transfusion.
What is the cost at DNA Labs India?
The HLA T & B Cell Lymphocyte Crossmatch test costs Rs 10000 at DNA Labs India.
Is home sample collection available?
Yes, free home sample collection is available for online bookings at DNA Labs India across major Indian cities.
What sample is required?
Recipient sample requires 6 mL whole blood in a Yellow Top ACD tube and 3 mL serum in an SST. Donor sample requires 12 mL whole blood in 2 Yellow Top ACD tubes.
Do I need to fast before this test?
No fasting is required for HLA T & B Cell Lymphocyte Crossmatch test.
How long do reports take?
Reports are typically available within 10-12 days after sample receipt.
What does a positive crossmatch mean?
A positive crossmatch indicates that recipient antibodies reacted with donor cells. This may increase the risk of rejection and needs further immunological evaluation.
What does a negative crossmatch mean?
A negative crossmatch suggests no significant donor-specific antibody reaction was detected under the tested conditions.
What are mandatory documents for the test?
A doctor's prescription on letterhead with stamp and one photograph each of recipient and donor are mandatory. A brief history in Solid Organ Transplant Consent form Form 33 is required when applicable.
Are there any risks?
Risks are minimal and may include mild pain, bruising, slight bleeding, or light-headedness at the venipuncture site.
How can I book this test?
You can book online through DNA Labs India website or contact the laboratory. Home sample collection is available for online bookings.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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