Immunohistochemistry Any 3 Markers Test
Short Name: IHC Any 3 Markers
Also known as: IHC 3 Markers Test, IHC Panel Test, Immunohistochemistry Panel, Tissue Protein Marker Test
Immunohistochemistry Any 3 Markers Test test available at DNA Labs India for ₹7,500. Uses Immunohistochemistry (IHC) on Tumor tissue in 10% Formal-saline OR Formalin Fixed Paraffin Embedded (FFPE) block samples. Results in Results are typically available within 5 working days for standard biopsy specimens and within 7 working days for large or complex tissue specimens.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The IHC Any 3 Markers Test is performed to identify and characterise specific proteins in tissue samples. It helps pathologists and oncologists confirm a diagnosis, determine the origin and type of a tumour, guide treatment decisions such as targeted therapy or immunotherapy, assess disease prognosis, and monitor treatment response. The test is also valuable in diagnosing certain autoimmune and infectious diseases where tissue-based evidence is required.
- Test Code
- 960
- Price
- ₹7,500
- Sample Type
- Tumor tissue in 10% Formal-saline OR Formalin Fixed Paraffin Embedded (FFPE) block
- Result Time
- Results are typically available within 5 working days for standard biopsy specimens and within 7 working days for large or complex tissue specimens.
- Fasting Required
- No
- Method
- Immunohistochemistry (IHC)
Sample Collection
The biopsy or surgical specimen should be promptly placed in 10% formal-saline or formalin. Provide a copy of the histopathology report, site of biopsy, and clinical history alongside the sample.
Method: Surgical biopsy or core needle biopsy (collected by treating physician)
Laboratory Analysis
Specimen collection (biopsy or surgery) is performed by the treating physician or surgeon at a hospital or clinic. No additional patient-side collection is required for this test.
Report Delivery
Ensure the specimen container is properly labelled with patient details. The tissue sample is then processed, embedded in paraffin, sectioned, and stained with the selected antibodies in the laboratory.
Timeline: Results are typically available within 5 working days for standard biopsy specimens and within 7 working days for large or complex tissue specimens.
Patient Instructions
About This Test
Who Should Get This Test
The IHC Any 3 Markers Test is performed to identify and characterise specific proteins in tissue samples. It helps pathologists and oncologists confirm a diagnosis, determine the origin and type of a tumour, guide treatment decisions such as targeted therapy or immunotherapy, assess disease prognosis, and monitor treatment response. The test is also valuable in diagnosing certain autoimmune and infectious diseases where tissue-based evidence is required.
How to Prepare
- Submit tumor tissue in 10% Formal-saline or as a Formalin Fixed Paraffin Embedded (FFPE) block
- Ship the specimen at room temperature
- Provide a copy of the histopathology report along with the sample
- Mention the site of biopsy clearly on the request form
- Include relevant clinical history and suspected diagnosis on the requisition form
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"Immunohistochemistry is an indispensable tool in modern oncology. Selecting the right three markers based on the clinical context and histopathological findings allows us to accurately subtype tumours, determine prognosis, and identify actionable therapeutic targets. I always recommend this test when histopathology alone is insufficient to arrive at a definitive diagnosis or when treatment decisions depend on specific protein expression patterns in the tissue. Patients should ensure that their biopsy specimen is properly preserved in formalin and that the histopathology report accompanies the sample for optimal marker selection and interpretation."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Specimen not preserved in formalin or received unfixed with significant autolysis
- Specimen container without proper patient identification or labelling
- Tissue sample too small to allow three marker staining
- Absence of clinical history, biopsy site, or histopathology report
- Specimen received in expired or inappropriate fixative
Understanding Your Results
The tissue expresses all three selected proteins. The combination of positivity helps confirm a specific diagnosis, identify tumour subtype, or guide targeted treatment. Clinical correlation is essential.
Partial positivity may indicate a specific tumour subtype or disease pattern. Additional markers or molecular tests may be recommended by the pathologist for a definitive conclusion.
A single positive marker may provide supportive evidence but may not be sufficient for a definitive diagnosis. Further clinical and pathological correlation is advised.
Negative staining for all three markers does not completely rule out disease. The pathologist may recommend repeat testing with a different marker panel or alternative diagnostic approaches.
Consult your oncologist or treating physician if your biopsy report is inconclusive, if you have been advised to undergo IHC testing for disease confirmation, if treatment decisions depend on marker results, or if you experience symptoms such as unexplained lumps, persistent fatigue, unexplained weight loss, or other signs suggestive of cancer or autoimmune disease.
Limitations
- ⚠IHC is a qualitative to semi-quantitative technique; it does not provide precise quantification of protein levels
- ⚠Results are subject to interpretation by the pathologist and may vary with antibody clones and protocols
- ⚠The test evaluates only the three selected markers; additional markers may be needed for a comprehensive assessment
- ⚠IHC cannot replace molecular or genetic testing for certain diagnostic and therapeutic decisions
- ⚠False positives and false negatives can occur due to technical or biological factors
Risks & Considerations
- ●IHC testing on tissue specimens is a non-invasive laboratory procedure and poses no direct risk to the patient
- ●Risks associated with the biopsy or surgery itself (e.g., bleeding, infection) are separate and should be discussed with the treating physician
- ●There is a small possibility of inconclusive results requiring repeat testing or additional markers
Interfering Factors
- ●Prolonged fixation time in formalin may degrade antigen epitopes, leading to false-negative results
- ●Inadequate fixation can cause tissue autolysis and unreliable staining
- ●Decalcification of bone tissue specimens may adversely affect antigen preservation
- ●Previous treatment with certain chemotherapeutic agents may alter protein expression
- ●Use of inappropriate fixatives other than 10% neutral buffered formalin
Compare With Similar Tests
| Test | Immunohistochemistry Any 3 Markers Test | IHC Any 1 Marker | IHC Any 2 Markers | IHC Any 5 Markers | IHC Any 10 Markers |
|---|---|---|---|---|---|
| Comparison | Immunohistochemistry Any 3 Markers Test |
Frequently Asked Questions
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