Immunohistochemistry CD5 Test
Short Name: IHC CD5
Also known as: CD5 Immunostain, IHC CD5 Stain, CD5 Antibody Test, CD5 Marker Immunohistochemistry
Immunohistochemistry CD5 Test test available at DNA Labs India for ₹3,000. Uses Immunohistochemistry (IHC) on Tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded (FFPE) block samples. Results in Sample Daily by 6 pm; Report - FFPE Block: 5 working days, Tissue Biopsy: 5 working days, Tissue large complex: 7 working days. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The Immunohistochemistry CD5 test is performed to detect aberrant CD5 expression in tissue samples, which aids in the diagnosis and sub-classification of B-cell and T-cell lymphoproliferative disorders. CD5 positivity in B-cell lymphomas supports a diagnosis of mantle cell lymphoma or CLL/SLL, while its expression on T cells confirms T-cell lineage. The test helps differentiate between various lymphoma subtypes, which is critical for determining the appropriate treatment protocol and predicting patient outcomes.
- Test Code
- 932
- CPT Code
- 88342
- ICD Code
- C83.0, C91.1
- Price
- ₹3,000
- Sample Type
- Tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded (FFPE) block
- Result Time
- Sample Daily by 6 pm; Report - FFPE Block: 5 working days, Tissue Biopsy: 5 working days, Tissue large complex: 7 working days
- Fasting Required
- No
- Method
- Immunohistochemistry (IHC)
Sample Collection
The tissue biopsy or surgical specimen must be properly fixed in 10% formal-saline or processed into a formalin-fixed paraffin-embedded (FFPE) block prior to submission. A copy of the histopathology report must be provided along with the site of biopsy and relevant clinical history. No special patient preparation such as fasting is required for this test as it is performed on an already collected tissue specimen.
Method: Tissue Biopsy / Surgical Excision
Laboratory Analysis
The tissue sample is collected via biopsy (excisional, incisional, or core needle) or surgical excision by the treating physician. The specimen is immediately placed in 10% neutral buffered formalin for fixation. The tissue is then processed, embedded in paraffin, and sectioned onto glass slides for immunohistochemical staining in the laboratory.
Report Delivery
After the immunohistochemical staining is completed, the stained slides are examined by a qualified pathologist who interprets the CD5 expression pattern, intensity, and percentage of positive cells. The results are compiled into a detailed report and made available within 5 to 7 working days via online portal, email, or WhatsApp.
Timeline: Sample Daily by 6 pm; Report - FFPE Block: 5 working days, Tissue Biopsy: 5 working days, Tissue large complex: 7 working days
Patient Instructions
About This Test
Who Should Get This Test
The Immunohistochemistry CD5 test is performed to detect aberrant CD5 expression in tissue samples, which aids in the diagnosis and sub-classification of B-cell and T-cell lymphoproliferative disorders. CD5 positivity in B-cell lymphomas supports a diagnosis of mantle cell lymphoma or CLL/SLL, while its expression on T cells confirms T-cell lineage. The test helps differentiate between various lymphoma subtypes, which is critical for determining the appropriate treatment protocol and predicting patient outcomes.
How to Prepare
- Submit tumor tissue in 10% Formal-saline or as a Formalin Fixed Paraffin Embedded (FFPE) block
- Ship the specimen at room temperature
- Provide a copy of the histopathology report with the specimen
- Indicate the site of biopsy on the requisition form
- Provide relevant clinical history including suspected diagnosis
- Ensure tissue is fixed for a minimum of 6 hours and not more than 72 hours in formalin
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"CD5 immunohistochemistry is a critical ancillary study in the workup of B-cell lymphomas. Aberrant CD5 expression on neoplastic B cells is a hallmark of mantle cell lymphoma and chronic lymphocytic leukemia/small lymphocytic lymphoma. It is essential to interpret CD5 staining in conjunction with other markers such as CD23, Cyclin D1, and CD20 for accurate subclassification and appropriate treatment planning."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Specimen received without formalin fixation or in dried condition
- Tissue sample too small for adequate immunohistochemical evaluation
- No clinical history or biopsy site information provided
- Specimen container leaked or improperly labeled
- Grossly necrotic tissue with no viable tumor cells identified
Understanding Your Results
Aberrant CD5 expression on B cells is characteristic of mantle cell lymphoma (MCL) and chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). Further testing with Cyclin D1, CD23, and SOX11 is recommended to differentiate between these entities.
CD5 expression on T cells is a normal finding. However, loss of CD5 expression on T cells may suggest a T-cell lymphoma such as peripheral T-cell lymphoma not otherwise specified (PTCL-NOS) or anaplastic large cell lymphoma.
Absence of CD5 on B cells suggests a B-cell lymphoma other than MCL or CLL/SLL, such as follicular lymphoma, marginal zone lymphoma, or diffuse large B-cell lymphoma (most cases). Further panel testing is required for definitive classification.
Loss of CD5 on T cells is abnormal and may indicate certain T-cell lymphomas. Correlation with additional T-cell markers (CD2, CD3, CD7) and clinical context is essential.
You should consult an oncologist or hematologist if your biopsy results show abnormal CD5 expression, if you have been diagnosed with or are being evaluated for a lymphoproliferative disorder, or if you experience symptoms such as persistent unexplained lymphadenopathy, unexplained weight loss, night sweats, fever, or fatigue. Early consultation ensures timely diagnosis and initiation of appropriate treatment.
Limitations
- ⚠CD5 alone is not diagnostic and must be interpreted with a panel of immunohistochemical markers
- ⚠Cannot distinguish between reactive and neoplastic T-cell populations without additional markers
- ⚠Rare cases of CD5-negative mantle cell lymphoma or CD5-positive diffuse large B-cell lymphoma exist
- ⚠Does not provide information about specific genetic translocations such as t(11;14) in MCL
- ⚠Results should always be correlated with clinical findings, morphology, and flow cytometry data
Risks & Considerations
- ●As the test is performed on an already excised tissue specimen, there are no additional procedural risks to the patient from the IHC test itself
- ●Risks associated with the original biopsy procedure may include minor bleeding, infection, or discomfort at the biopsy site
Interfering Factors
- ●Inadequate fixation of tissue sample may lead to false-negative results
- ●Prolonged fixation in formalin beyond 48-72 hours can degrade antigenicity
- ●Decalcification of bone marrow trephine biopsies may impair CD5 antigen detection
- ●Necrotic or heavily autolyzed tissue may yield unreliable staining
- ●Technical errors in antigen retrieval or antibody concentration may affect results
Compare With Similar Tests
| Test | Immunohistochemistry CD5 Test | CD23 | Cyclin D1 | SOX11 | CD20 |
|---|---|---|---|---|---|
| Comparison | Immunohistochemistry CD5 Test | CD23 is positive in CLL/SLL but negative in MCL, whereas CD5 is positive in both. CD23 helps differentiate between these two CD5-positive B-cell lymphomas. | Cyclin D1 is a hallmark of mantle cell lymphoma (t(11;14) translocation) and is positive in MCL but negative in CLL/SLL. Combined CD5+/Cyclin D1+ pattern strongly supports MCL. | SOX11 is a nuclear transcription factor marker for mantle cell lymphoma. It is useful when Cyclin D1 is equivocal and complements CD5 in MCL diagnosis. | CD20 is a pan B-cell marker. CD5 is evaluated on CD20-positive B-cell populations to determine aberrant co-expression, which narrows the differential diagnosis. |
Frequently Asked Questions
What is the Immunohistochemistry CD5 Test?
Why is the CD5 test ordered by my doctor?
What sample is required for the Immunohistochemistry CD5 Test?
What is the cost of the Immunohistochemistry CD5 Test at DNA Labs India?
How long does it take to get the CD5 test results?
Does the CD5 test require fasting?
What does a positive CD5 result on B cells mean?
What does a negative CD5 result mean?
Is the CD5 test the only test needed to diagnose lymphoma?
Is home sample collection available for the CD5 test?
Can the CD5 test detect all types of lymphoma?
Is the Immunohistochemistry CD5 Test covered by insurance in India?
Related Tests
Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
Book Your Test
Enter your details and we'll connect you within 15 minutes.