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Immunohistochemistry CD99 (MIC-2) Test

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Immunohistochemistry CD99 (MIC-2) Test

Short Name: CD99 (MIC-2) IHC Test

Also known as: MIC-2, CD99 protein

Immunohistochemistry CD99 (MIC-2) Test test available at DNA Labs India for ₹3,000. Uses Immunohistochemistry on Tumor tissue samples. Results in Reports are typically available within 5 to 7 days after sample receipt.. Free home collection in 300+ cities across India.

Immunohistochemistry🏠 Home Collection

🩺 Medically Reviewed By

Overview

The primary purpose of the Immunohistochemistry CD99 (MIC-2) test is to assist in the diagnosis of Ewing's sarcoma and other tumors expressing CD99 protein. It is used to differentiate small round cell tumors, confirm malignancy, and support treatment planning in oncology cases.

Test Code
927
Price
₹3,000
Sample Type
Tumor tissue
Result Time
Reports are typically available within 5 to 7 days after sample receipt.
Fasting Required
No
Method
Immunohistochemistry
Step 1

Sample Collection

Provide a copy of the histopathology report, site of biopsy, and clinical history. No special preparation is required from the patient.

Method: Biopsy or surgical procedure

Step 2

Laboratory Analysis

Sample is obtained via biopsy or surgical procedure performed by a healthcare professional.

Step 3

Report Delivery

Tissue sample is preserved in formalin or saline and shipped at room temperature to the laboratory.

Timeline: Reports are typically available within 5 to 7 days after sample receipt.

Patient Instructions

1
Before the Test:Ensure all clinical history and prior test reports are available for the laboratory.
2
During the Test:The test involves staining tissue samples with CD99 antibodies in a lab setting; no patient involvement required during testing.
3
After the Test:Results are analyzed by a pathologist and delivered as per the chosen method. Follow-up with a doctor is recommended for interpretation.

About This Test

Who Should Get This Test

The primary purpose of the Immunohistochemistry CD99 (MIC-2) test is to assist in the diagnosis of Ewing's sarcoma and other tumors expressing CD99 protein. It is used to differentiate small round cell tumors, confirm malignancy, and support treatment planning in oncology cases.

How to Prepare

  • Submit tumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded block
  • Ship at room temperature
  • Provide a copy of the Histopathology report
  • Include site of biopsy and clinical history

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This test is crucial for accurate diagnosis and management of Ewing's sarcoma, helping to differentiate it from other small round cell tumors and guide treatment decisions."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeTumor tissue
Sample VolumeAs required for biopsy
Container10% Formal-saline or Formalin fixed paraffin embedded block
Collection MethodBiopsy or surgical procedure

Sample Stability

Room temperature: Stable during shipping
Refrigerator: Not typically required
Frozen: Not applicable
Sample Rejection Criteria:
  • Inadequate tissue sample
  • Improper fixation or preservation
  • Missing clinical information
  • Contaminated or degraded tissue

Understanding Your Results

Results from the CD99 (MIC-2) test are interpreted by a pathologist to determine the presence and extent of CD99 protein expression in the tissue sample.
Positive Result: CD99 protein detected, suggestive of Ewing's sarcoma or related tumors; requires correlation with clinical and other diagnostic findings.
Negative Result: No CD99 protein detected; does not rule out cancer but may indicate other tumor types.
Staining Intensity: Helps assess expression levels, with strong staining often associated with higher diagnostic confidence.
Clinical Correlation: Results should be integrated with imaging, history, and other tests for accurate diagnosis.
⚠️ When to Consult a Doctor:

Consult an oncologist or specialist if you experience persistent bone pain, swelling, fever, unexplained weight loss, or if biopsy results indicate small round cell tumors. Early consultation is crucial for timely diagnosis and treatment.

Limitations

  • False negatives possible if CD99 expression is low
  • Requires expert pathologist interpretation
  • Not a standalone diagnostic tool; must be correlated with clinical findings
  • Limited to protein detection; does not assess genetic mutations

Risks & Considerations

  • Risks associated with biopsy procedure, such as infection or bleeding
  • Potential for false-negative or false-positive results
  • Need for repeat testing in ambiguous cases

Interfering Factors

  • Poor tissue fixation or preservation
  • Inadequate sample size
  • Use of improper staining techniques
  • Tissue necrosis or autolysis
  • Presence of interfering substances in tissue

Compare With Similar Tests

TestImmunohistochemistry CD99 (MIC-2) Test
ComparisonImmunohistochemistry CD99 (MIC-2) Test

Frequently Asked Questions

What is the CD99 (MIC-2) test?
It is an immunohistochemistry test that detects CD99 protein in tissue samples, commonly used to diagnose Ewing's sarcoma.
Why is this test recommended?
It is recommended for differential diagnosis of small round cell tumors, especially in suspected Ewing's sarcoma cases.
What are the symptoms of Ewing's sarcoma?
Symptoms include localized pain, swelling, fever, fatigue, and weight loss in the affected area.
How is the test performed?
A tissue sample from biopsy or surgery is stained with CD99 antibodies and examined under a microscope.
What is the cost of the CD99 test in India?
The cost is INR 3000, including home collection services across many cities.
Is home sample collection available?
Yes, DNA Labs India offers free home collection for this test in numerous locations.
How long does it take to get results?
Results are usually available within 5 to 7 days after sample receipt.
What does a positive result indicate?
A positive result suggests CD99 expression, which is typical in Ewing's sarcoma, but requires correlation with clinical findings.
Are there any risks to this test?
The test itself has no direct risks, but the biopsy procedure may have minor risks like infection or bleeding.
Do I need to fast before this test?
No, fasting is not required as it is a tissue-based test.
What is the accuracy of the CD99 test?
It is highly accurate when performed correctly, but results should be interpreted alongside other diagnostic information.
Can this test detect other cancers?
CD99 is primarily associated with Ewing's sarcoma but may be expressed in other tumors; it is not specific to all cancers.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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