Immunohistochemistry H3K27M / H3K27me3 Test
Short Name: IHC H3K27M/H3K27me3
Also known as: IHC H3K27M/H3K27me3, H3K27M Mutation Test, H3K27me3 Loss Test
Immunohistochemistry H3K27M / H3K27me3 Test test available at DNA Labs India for ₹3,500. Uses Immunohistochemistry on Tumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded block samples. Results in Report Block: 5 days, Tissue Biopsy: 5 days, Tissue large complex: 7 days. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
To detect H3K27M mutations and H3K27me3 loss in tumor tissue for the diagnosis and management of central nervous system tumors.
- Test Code
- 902
- Price
- ₹3,500
- Sample Type
- Tumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded block
- Result Time
- Report Block: 5 days, Tissue Biopsy: 5 days, Tissue large complex: 7 days
- Fasting Required
- No
- Method
- Immunohistochemistry
Sample Collection
Provide a copy of the histopathology report, site of biopsy, and clinical history.
Method: Tissue Biopsy
Laboratory Analysis
Tissue biopsy procedure performed by a specialist, preserving sample in appropriate fixative.
Report Delivery
Ship sample at room temperature to the laboratory for analysis.
Timeline: Report Block: 5 days, Tissue Biopsy: 5 days, Tissue large complex: 7 days
Patient Instructions
About This Test
Who Should Get This Test
To detect H3K27M mutations and H3K27me3 loss in tumor tissue for the diagnosis and management of central nervous system tumors.
How to Prepare
- Submit tumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded block
- Ship at room temperature
- Provide a copy of the histopathology report, site of biopsy, and clinical history
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This test is critical for accurate diagnosis and targeted treatment planning in central nervous system tumors, particularly diffuse midline gliomas."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Improperly fixed tissue
- Insufficient sample size
- Missing clinical information
Understanding Your Results
Positive for H3K27M
Suggestive of diffuse midline glioma or related CNS tumors
Loss of H3K27me3
Indicates epigenetic alteration associated with aggressive tumor behavior
Negative for both
No evidence of H3K27M mutation or H3K27me3 loss
If symptoms persist or if test results indicate presence of mutations, consult an oncologist for further management.
Limitations
- ⚠May not detect all H3K27M mutations due to tumor heterogeneity
- ⚠Requires experienced pathologist for interpretation
- ⚠Limited to tissue samples, not applicable to blood or fluid samples
Risks & Considerations
- ●Minimal risks from biopsy procedure, such as infection or bleeding
- ●Rare allergic reactions to fixatives
Interfering Factors
- ●Improper tissue fixation
- ●Tissue degradation or necrosis
- ●Inadequate biopsy sample
- ●Technical errors in staining procedure
Compare With Similar Tests
| Test | Immunohistochemistry H3K27M / H3K27me3 Test | EGFR Mutation Analysis | IDH1/IDH2 Mutation Test | MGMT Promoter Methylation Test |
|---|---|---|---|---|
| Comparison | Immunohistochemistry H3K27M / H3K27me3 Test |
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