Immunohistochemistry LIN28 Test
Short Name: IHC LIN28
Also known as: LIN28 IHC Test, LIN28 Protein Detection Test, IHC LIN28 Staining
Immunohistochemistry LIN28 Test test available at DNA Labs India for ₹3,500. Uses Immunohistochemistry on Tumor tissue samples. Results in Sample accepted daily by 6 pm. Report Block: 5 days. Tissue Biopsy: 5 days. Tissue large complex: 7 days.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The purpose of the Immunohistochemistry LIN28 test is to detect and evaluate the expression of LIN28 protein in tumour tissue specimens. This helps oncologists and pathologists in the diagnosis, classification, and prognostic assessment of certain cancers, especially paediatric solid tumours such as neuroblastoma, Wilms tumour, and hepatoblastoma. The test serves as an important ancillary marker that supports clinical decision-making and treatment planning.
- Test Code
- 1012
- Price
- ₹3,500
- Sample Type
- Tumor tissue
- Result Time
- Sample accepted daily by 6 pm. Report Block: 5 days. Tissue Biopsy: 5 days. Tissue large complex: 7 days.
- Fasting Required
- No
- Method
- Immunohistochemistry
Sample Collection
A tissue biopsy must be performed by a qualified surgeon or interventional radiologist. Provide a copy of the histopathology report, site of biopsy, and clinical history with the sample.
Method: Tissue biopsy
Laboratory Analysis
The biopsy tissue is collected and immediately placed in 10% formal-saline or processed into a formalin fixed paraffin embedded (FFPE) block as per standard histopathology protocols.
Report Delivery
The sample is transported to the laboratory at room temperature. Ensure the specimen is labelled correctly with patient details and accompanied by the required clinical documentation.
Timeline: Sample accepted daily by 6 pm. Report Block: 5 days. Tissue Biopsy: 5 days. Tissue large complex: 7 days.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the Immunohistochemistry LIN28 test is to detect and evaluate the expression of LIN28 protein in tumour tissue specimens. This helps oncologists and pathologists in the diagnosis, classification, and prognostic assessment of certain cancers, especially paediatric solid tumours such as neuroblastoma, Wilms tumour, and hepatoblastoma. The test serves as an important ancillary marker that supports clinical decision-making and treatment planning.
How to Prepare
- Submit tumour tissue in 10% formal-saline or as a formalin fixed paraffin embedded (FFPE) block
- Ship the sample at room temperature
- Provide a copy of the histopathology report with the sample
- Include the site of biopsy information
- Include relevant clinical history for accurate interpretation
- Ensure the sample container is properly labelled with patient identification
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"The Immunohistochemistry LIN28 test is a valuable ancillary diagnostic tool in the evaluation of certain pediatric and adult tumors. LIN28 overexpression has been observed in neuroblastoma, Wilms tumor, and hepatoblastoma among others. A positive LIN28 result should always be interpreted in the context of histomorphology, clinical presentation, and other ancillary studies. Timely testing and expert pathological review are critical for accurate diagnosis and appropriate treatment planning. Patients presenting with unexplained abdominal swelling, weight loss, or other cancer-related symptoms should consult an oncologist promptly."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Tissue not fixed in appropriate fixative (10% formal-saline or formalin)
- Sample without proper patient identification or labelling
- Missing histopathology report, site of biopsy, or clinical history
- Excessively autolysed or degraded tissue sample
- Sample received without required documentation
Understanding Your Results
Positive
LIN28 protein expression detected. This may indicate association with certain malignancies such as neuroblastoma, Wilms tumour, or hepatoblastoma. Clinical correlation and further workup recommended.
Negative
No LIN28 protein expression detected. This does not entirely exclude malignancy. Clinical correlation with other diagnostic findings is necessary.
Consult an oncologist if you experience any of the following symptoms: unexplained abdominal swelling or pain, unexplained weight loss, loss of appetite, persistent fatigue, fever, bone pain, persistent cough, or difficulty breathing. Early consultation and timely diagnostic testing are essential for accurate diagnosis and effective treatment. If your IHC LIN28 test result is positive, consult your oncologist promptly for further evaluation and treatment planning.
Limitations
- ⚠This test is qualitative and does not quantify the level of LIN28 expression
- ⚠Results must be interpreted in conjunction with clinical findings and other diagnostic tests
- ⚠A negative result does not completely rule out the presence of cancer
- ⚠Cross-reactivity with related proteins is possible, though rare with validated antibodies
- ⚠Test performance may vary depending on tissue type and processing conditions
Risks & Considerations
- ●The IHC LIN28 test itself carries no direct risk as it is a laboratory analysis performed on an already-collected tissue sample
- ●Risks are associated with the biopsy procedure used to collect the tissue sample, which should be discussed with your physician
Interfering Factors
- ●Inadequate or improperly preserved tissue sample
- ●Prolonged fixation time in formalin affecting antigen integrity
- ●Tissue necrosis or excessive blood contamination in the specimen
- ●Use of decalcifying agents that may degrade antigens
Compare With Similar Tests
| Test | Immunohistochemistry LIN28 Test | Immunohistochemistry (IHC) Panel for Neuroblastoma | Tissue Biopsy – Histopathology | FNAC (Fine Needle Aspiration Cytology) |
|---|---|---|---|---|
| Comparison | Immunohistochemistry LIN28 Test | The IHC LIN28 test specifically detects LIN28 protein, while a broader neuroblastoma IHC panel includes multiple markers such as NSE, chromogranin, synaptophysin, and CD56 for comprehensive tumour characterisation. | Standard histopathology examines tissue architecture and cell morphology, while the IHC LIN28 test provides molecular-level protein detection for specific diagnostic and prognostic information. | FNAC provides a cytological assessment of a tumour, while IHC LIN28 is performed on tissue biopsy specimens to detect specific protein expression at the tissue level. |
Frequently Asked Questions
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