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Immunophenotyping by Flow Cytometry Kappa & Lambda Light Chains Test

DNA Labs India | ISO 9001:2015 Certified

Immunophenotyping by Flow Cytometry Kappa & Lambda Light Chains Test

Short Name: Kappa Lambda Light Chain Flow Cytometry

Also known as: Kappa/Lambda Light Chain Flow Cytometry, B Cell Clonality Assay, Immunoglobulin Light Chain Clonality by Flow Cytometry

Immunophenotyping by Flow Cytometry Kappa & Lambda Light Chains Test test available at DNA Labs India for ₹5,500. Uses Flow Cytometry on Whole blood and bone marrow samples. Results in Sample accepted daily by 9 am; report available on the same day.. Free home collection in 300+ cities across India.

Oncologist, HematologistAll age groups🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

To detect clonal B-cell populations by assessing kappa and lambda immunoglobulin light chain expression and to evaluate B-cell maturation in suspected hematological malignancies.

Test Code
3489
Price
₹5,500
Sample Type
Whole blood and bone marrow
Result Time
Sample accepted daily by 9 am; report available on the same day.
Fasting Required
No
Method
Flow Cytometry
Step 1

Sample Collection

Give a brief clinical history. No fasting is required. Specify time, date, and clinical details on the test request form.

Method: Venipuncture or bone marrow aspiration

Step 2

Laboratory Analysis

Collect 3 mL whole blood in EDTA and sodium heparin tubes, or 2 mL bone marrow in sodium heparin. Mix gently and label correctly.

Step 3

Report Delivery

Ship immediately at 18–22°C. Do not refrigerate or freeze.

Timeline: Sample accepted daily by 9 am; report available on the same day.

Patient Instructions

1
Before the Test:No fasting is required. Inform the laboratory of any relevant clinical history or ongoing medications.
2
During the Test:A blood sample or bone marrow sample is collected by a trained professional.
3
After the Test:No special restrictions are needed. You can resume normal activities unless advised otherwise.

About This Test

Who Should Get This Test

To detect clonal B-cell populations by assessing kappa and lambda immunoglobulin light chain expression and to evaluate B-cell maturation in suspected hematological malignancies.

How to Prepare

  • Collect 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube.
  • Collect 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube.
  • Alternatively, collect 2 mL (1 mL min.) bone marrow in 1 Green Top (Sodium Heparin) tube.
  • Ship immediately at 18–22°C. Do not refrigerate or freeze.
  • Specify time, date, and clinical details on the test request form.

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"Flow cytometry for kappa and lambda light chains helps confirm clonal B-cell populations. Always interpret the result with morphology, complete blood counts, and clinical findings."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeWhole blood and bone marrow
Sample Volume3 mL whole blood in EDTA, 3 mL whole blood in sodium heparin, or 2 mL bone marrow in sodium heparin
ContainerLavender Top EDTA tube and Green Top Sodium Heparin tube
Collection MethodVenipuncture or bone marrow aspiration

Sample Stability

Room Temperature
Refrigerator
Frozen
Sample Rejection Criteria:
  • Clotted sample
  • Underfilled sample
  • Frozen or refrigerated sample
  • Hemolyzed sample

Understanding Your Results

Flow cytometry interpretation evaluates the kappa and lambda light chain expression pattern on B cells. A polyclonal pattern is typically seen in normal or reactive conditions, whereas a clonal or restricted pattern suggests a clonal B-cell population. Results must be correlated with morphology, clinical history, and other markers.
Polyclonal kappa/lambda expression: usually reactive or normal B-cell population.
Clonal or restricted kappa/lambda expression: suggests clonal B-cell proliferation; needs clinical and laboratory correlation.
Absent surface light chain may be seen in certain immature B cells or plasma cells; interpret with B-cell maturity markers.
⚠️ When to Consult a Doctor:

Consult your doctor if the report shows clonal B-cell population or if you have persistent fever, unexplained weight loss, enlarged lymph nodes, abnormal blood counts, or other concerning symptoms.

Limitations

  • ⚠Flow cytometry results must be correlated with clinical history and morphology.
  • ⚠A polyclonal pattern does not completely exclude a clonal process if the sample is paucicellular.
  • ⚠Requires viable cells and adequate cell count.

Risks & Considerations

  • ●Mild pain or bruising at the venipuncture site
  • ●Transient discomfort with bone marrow collection
  • ●Rare bleeding or infection at the collection site

Interfering Factors

  • ●Hemolyzed, clotted, or insufficient sample
  • ●Delayed or improper transport
  • ●Recent chemotherapy or B-cell-directed therapy
  • ●Refrigerated or frozen sample

Compare With Similar Tests

TestImmunophenotyping by Flow Cytometry Kappa & Lambda Light Chains Test
ComparisonImmunophenotyping by Flow Cytometry Kappa & Lambda Light Chains Test

Frequently Asked Questions

What is Immunophenotyping by Flow Cytometry Kappa & Lambda Light Chains Test?
It is a flow cytometry-based test that evaluates kappa and lambda immunoglobulin light chain expression on B cells to identify clonal B-cell populations and help classify blood cancers.
Why is the test performed?
It is used to evaluate suspected leukemia, lymphoma, myeloma, and other B-cell lymphoproliferative disorders.
What sample is required?
3 mL whole blood in EDTA and sodium heparin tubes, or 2 mL bone marrow in sodium heparin.
Is fasting required before the test?
No fasting is required; give a brief clinical history.
How should the sample be transported?
Ship immediately at 18–22°C; do not refrigerate or freeze.
When will I receive the report?
Sample is accepted daily by 9 am, and reports are available the same day.
What does clonal restriction mean?
It means B cells show a predominant kappa or lambda light chain pattern, suggesting a clonal B-cell proliferation.
What is the cost of this test?
It costs Rs 5500 at DNA Labs India.
Is home sample collection available?
Yes, free home sample collection is available for online bookings across India.
What conditions can this test help diagnose?
It aids in the diagnosis and classification of leukemia, lymphoma, myeloma, and related B-cell disorders.
Are there any risks?
Risks are minimal and may include mild bruising or discomfort; bone marrow collection can cause transient pain.
Can I book this test online?
Yes, it can be booked online on the DNA Labs India website.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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