Immunophenotyping by Flow Cytometry: Leukemia Diagnostic Comprehensive Profile Any 6 Markers Test
Short Name: Leukemia Flow Cytometry 6-Marker Profile
Also known as: Leukemia Diagnostic Comprehensive Profile Any 6 Markers, Acute Leukemia Immunophenotyping Panel, Flow Cytometry Leukemia Panel
Immunophenotyping by Flow Cytometry: Leukemia Diagnostic Comprehensive Profile Any 6 Markers Test test available at DNA Labs India for ₹20,000. Uses Flow Cytometry, Culture, Microscopy, Karyotype, Real Time PCR on Bone Marrow Aspirate / Whole Blood samples. Results in Sample accepted daily by 11 am; report delivered in 7 working days.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
Leukemia diagnostic panel, acute leukemia lineage (T, B or myeloid); chromosome analysis for hematological malignancy; leukemia genetic profile with any 6 markers by PCR.
- Test Code
- 3500
- Price
- ₹20,000
- Sample Type
- Bone Marrow Aspirate / Whole Blood
- Result Time
- Sample accepted daily by 11 am; report delivered in 7 working days.
- Fasting Required
- No
- Method
- Flow Cytometry, Culture, Microscopy, Karyotype, Real Time PCR
Sample Collection
Duly filled Chromosome & FISH analysis requisition form Form 17 is mandatory. No fasting is required. Inform the laboratory about any recent chemotherapy, growth factor therapy, or blood transfusion.
Method: Venipuncture or bone marrow aspiration
Laboratory Analysis
Collect 4 mL (3 mL min.) bone marrow aspirate or whole blood in a green top sodium heparin tube and 3 mL (2 mL min.) whole blood or bone marrow aspirate in a lavender top EDTA tube. Mix gently by inversion. Do not refrigerate or freeze.
Report Delivery
Ship immediately at 18–22°C. Do not refrigerate or freeze. Ensure the completed Form 17 requisition form accompanies the sample.
Timeline: Sample accepted daily by 11 am; report delivered in 7 working days.
Patient Instructions
About This Test
Who Should Get This Test
Leukemia diagnostic panel, acute leukemia lineage (T, B or myeloid); chromosome analysis for hematological malignancy; leukemia genetic profile with any 6 markers by PCR.
How to Prepare
- Use a green top sodium heparin tube and a lavender top EDTA tube
- Collect 4 mL bone marrow aspirate or whole blood in sodium heparin
- Collect 3 mL whole blood or bone marrow aspirate in EDTA
- Mix tubes gently by inversion
- Ship immediately at 18–22°C; do not refrigerate or freeze
- Attach duly filled Chromosome & FISH analysis requisition form Form 17
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"Flow cytometry immunophenotyping is central to classifying acute leukemia into T, B, or myeloid lineage. The choice of any six markers should be guided by clinical morphology, blast count, and suspected lineage to avoid diagnostic delay and to plan targeted cytogenetic or molecular testing."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Refrigerated or frozen sample
- Clotted specimen
- Incorrect sample container
- Insufficient sample volume
- Missing or incomplete Form 17 requisition form
- Delayed transport beyond stability
Understanding Your Results
Often positive for B-lineage markers such as CD10, CD19, and TdT.
Often positive for T-lineage markers such as CD3, CD5, or CD7.
Often positive for myeloid markers such as CD13, CD33, or MPO; CD34/HLA-DR may be variable.
Leukaemic blasts may express markers of more than one lineage; correlation with morphology, karyotype, FISH, and PCR is required.
Consult an oncologist or hematologist if you have persistent fatigue, unexplained fever, night sweats, weight loss, abnormal bleeding or bruising, enlarged lymph nodes, or if any diagnostic report suggests a hematological malignancy.
Limitations
- ⚠The panel is limited to any 6 selected markers; additional markers may be needed for complete lineage assignment
- ⚠Flow cytometry results must be correlated with morphology, cytogenetics, FISH, and molecular studies
- ⚠Not a standalone diagnostic test; requires hematopathologist interpretation
- ⚠Aberrant antigen expression may produce overlapping lineage features
Risks & Considerations
- ●Mild pain or bruising at the venipuncture site
- ●Discomfort at the bone marrow aspirate site
- ●Rare bleeding or infection at the collection site
Interfering Factors
- ●Hemodiluted bone marrow aspirate
- ●Clotted specimen
- ●Improper storage or transport; refrigeration or freezing
- ●Delay in sample transport beyond recommended stability
- ●Recent chemotherapy, steroids, or colony-stimulating factors
- ●Incomplete or missing Chromosome & FISH analysis requisition form Form 17
Compare With Similar Tests
| Test | Immunophenotyping by Flow Cytometry: Leukemia Diagnostic Comprehensive Profile Any 6 Markers Test | ||||
|---|---|---|---|---|---|
| Comparison | Immunophenotyping by Flow Cytometry: Leukemia Diagnostic Comprehensive Profile Any 6 Markers Test |
Frequently Asked Questions
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