Infliximab Anti-TNF Blocker Test
Short Name: Infliximab Drug Level
Also known as: Infliximab Drug Level Test, Infliximab Trough Level Test, Infliximab Quantitation Test, Anti-TNF Drug Monitoring Test
Infliximab Anti-TNF Blocker Test test available at DNA Labs India for ₹32,000. Uses ELISA (Enzyme-Linked Immunosorbent Assay) on Serum samples. Results in Reports are typically available within 2-3 weeks after sample collection. Samples collected by the 7th of the month are processed with results delivered after 2-3 weeks.. Free home collection in 300+ cities across India.
Overview
The Infliximab Anti-TNF Blocker Test is performed to measure the trough serum concentration of Infliximab in patients receiving anti-TNF therapy. This test helps clinicians evaluate whether a patient has achieved therapeutic drug levels, assess the cause of treatment failure or loss of response, optimize dosing strategies, detect the presence of anti-drug antibodies indirectly through low trough levels, and guide decisions regarding dose escalation, treatment switching, or discontinuation. It is an integral component of personalized medicine in the management of chronic inflammatory diseases.
- Test Code
- 1112
- CPT Code
- 80299
- ICD Code
- Z51.81
- Price
- ₹32,000
- Sample Type
- Serum
- Result Time
- Reports are typically available within 2-3 weeks after sample collection. Samples collected by the 7th of the month are processed with results delivered after 2-3 weeks.
- Fasting Required
- No
- Method
- ELISA (Enzyme-Linked Immunosorbent Assay)
Sample Collection
The patient should be scheduled for sample collection just before the next scheduled Infliximab dose (trough level). No fasting is required. Inform the laboratory of current medications including immunomodulators, corticosteroids, and the date and dose of the last Infliximab infusion.
Method: Venipuncture
Laboratory Analysis
A standard venipuncture will be performed to collect 3 mL of blood into a Serum Separator Tube (SST). The procedure typically takes less than 5 minutes.
Report Delivery
Apply gentle pressure to the venipuncture site. The sample will be processed, refrigerated or frozen as required, and dispatched to the laboratory for ELISA-based analysis.
Timeline: Reports are typically available within 2-3 weeks after sample collection. Samples collected by the 7th of the month are processed with results delivered after 2-3 weeks.
Patient Instructions
About This Test
Who Should Get This Test
The Infliximab Anti-TNF Blocker Test is performed to measure the trough serum concentration of Infliximab in patients receiving anti-TNF therapy. This test helps clinicians evaluate whether a patient has achieved therapeutic drug levels, assess the cause of treatment failure or loss of response, optimize dosing strategies, detect the presence of anti-drug antibodies indirectly through low trough levels, and guide decisions regarding dose escalation, treatment switching, or discontinuation. It is an integral component of personalized medicine in the management of chronic inflammatory diseases.
How to Prepare
- Schedule sample collection just before the next Infliximab infusion (trough level)
- No fasting is required prior to sample collection
- Provide complete medication history including immunomodulatory agents
- Note the date and dosage of the most recent Infliximab infusion
- Sample stability: Room temperature up to 4 hours, Refrigerated up to 7 days, Frozen up to 14 days
- Ship the sample refrigerated or frozen to maintain sample integrity
Doctor's Notes
"Infliximab trough level monitoring is essential for patients showing loss of response to anti-TNF therapy. Measuring drug trough concentrations helps clinicians distinguish between inadequate drug levels, which may require dose escalation, and immunogenic loss of response due to anti-drug antibodies, which may necessitate switching to an alternative biologic. This test guides personalized treatment decisions and improves long-term clinical outcomes in patients with inflammatory bowel disease, rheumatoid arthritis, and other inflammatory conditions."
Test Parameters & Specifications
Sample Stability
- Sample collected at incorrect time point (not at trough)
- Hemolyzed, lipemic, or grossly contaminated sample
- Sample collected in incorrect tube type (non-SST)
- Insufficient sample volume (less than 2 mL)
- Sample not stored or shipped at recommended temperature
- Unlabeled or mislabeled sample
Understanding Your Results
Low Infliximab trough levels in a patient with active disease suggest inadequate drug exposure. This may be due to accelerated drug clearance, immunogenic loss of response (presence of anti-drug antibodies), or insufficient dosing. Dose escalation, shortening of infusion intervals, or addition of an immunomodulator may be considered.
Clinical action: Consult treating gastroenterologist or rheumatologist for dose adjustment or therapy modification.
Level: Sub-therapeutic Trough Level
Adequate Infliximab trough levels indicate sufficient drug exposure. If the patient has active disease despite therapeutic drug levels, the inflammation may be driven by non-TNF pathways, and alternative therapeutic mechanisms should be explored.
Clinical action: Discuss with treating specialist regarding disease activity assessment and possible alternative biologic therapy.
Level: Therapeutic Trough Level
Unusually high Infliximab trough levels may suggest reduced drug clearance, particularly in patients with high albumin levels, or may be seen after dose adjustments. While not typically harmful, monitoring for infection risk and dose optimization may be warranted.
Clinical action: Review dosing schedule with treating physician. Monitor for signs of infection.
Level: Supra-therapeutic Trough Level
Consult your treating gastroenterologist, rheumatologist, or immunologist if your Infliximab trough levels are below the therapeutic range, if you experience worsening symptoms despite regular Infliximab infusions, if you develop new or unusual symptoms such as persistent fever, infections, or skin reactions during anti-TNF therapy, or if your test results require clinical correlation with disease activity markers.
Limitations
- ⚠This test measures total Infliximab drug levels and does not directly quantify anti-drug antibodies (ADA)
- ⚠Trough levels provide a single time-point measurement and may not fully reflect overall drug exposure
- ⚠Results should always be interpreted in conjunction with clinical assessment, C-reactive protein levels, and other relevant markers
- ⚠The therapeutic range may vary depending on the specific disease being treated and treatment goals
- ⚠ELISA-based assays may show variability between different assay platforms and kits
Risks & Considerations
- ●Minor bruising or discomfort at the venipuncture site
- ●Rare risk of slight dizziness or lightheadedness during blood draw
- ●Extremely rare risk of infection at the puncture site
Interfering Factors
- ●Timing of sample collection relative to the Infliximab infusion cycle can significantly affect measured levels
- ●Concurrent immunomodulatory therapy (e.g., azathioprine, methotrexate) may influence drug clearance and trough levels
- ●High levels of anti-drug antibodies (ADA) may interfere with the assay and lead to falsely low Infliximab levels
- ●Severe hypoalbuminemia can accelerate Infliximab clearance and alter trough concentrations
- ●Patient body mass index and gender may contribute to inter-individual variability in drug levels
- ●Sample hemolysis or lipemia may interfere with ELISA accuracy
Compare With Similar Tests
| Test | Infliximab Anti-TNF Blocker Test | C-Reactive Protein (CRP) | Anti-Infliximab Antibodies Test | Fecal Calprotectin |
|---|---|---|---|---|
| Comparison | Infliximab Anti-TNF Blocker Test |
Frequently Asked Questions
What is the Infliximab Anti-TNF Blocker Test?
Why do I need the Infliximab Anti-TNF Blocker Test?
What does a low Infliximab trough level mean?
What does a high or therapeutic Infliximab trough level mean?
When should the blood sample be collected for this test?
Do I need to fast before the Infliximab Anti-TNF Blocker Test?
What sample type is required for the Infliximab Anti-TNF Blocker Test?
What is the cost of the Infliximab Anti-TNF Blocker Test in India?
How long does it take to get the Infliximab test results?
What is the ELISA method used in this test?
Is home sample collection available for the Infliximab Anti-TNF Blocker Test?
What conditions are treated with Infliximab that may require this monitoring test?
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