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DNA Labs India

Insulin Response to Glucose 5 Hours Test

DNA Labs India | ISO 9001:2015 Certified

Insulin Response to Glucose 5 Hours Test

Short Name: Insulin Response Test 5H

Also known as: 5-hour glucose tolerance test with insulin, Extended glucose tolerance test, Insulin sensitivity test

Insulin Response to Glucose 5 Hours Test test available at DNA Labs India for ₹10,500. Uses Chemiluminescent Microparticle Immunoassay, Hexokinase on Plasma from Grey Top tube and Serum from SST samples. Results in Results available within 24-48 hours after test completion.. Free home collection in 300+ cities across India.

DiagnosticAdults and Children🏠 Home Collection

🩺 Medically Reviewed By

Overview

To assess insulin resistance and diagnose diabetes by measuring blood glucose and insulin levels over 5 hours after glucose ingestion.

Test Code
1113
Price
₹10,500
Sample Type
Plasma from Grey Top tube and Serum from SST
Result Time
Results available within 24-48 hours after test completion.
Fasting Required
Yes
Method
Chemiluminescent Microparticle Immunoassay, Hexokinase
Step 1

Sample Collection

Overnight fasting is mandatory for at least 8-12 hours before the test.

Method: Venipuncture

Step 2

Laboratory Analysis

Blood samples are drawn at baseline and at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, and 5.0 hours after glucose administration.

Step 3

Report Delivery

Resume normal activities and diet; monitor for any dizziness or discomfort and consult doctor if needed.

Timeline: Results available within 24-48 hours after test completion.

Patient Instructions

1
Before the Test:Ensure overnight fasting and avoid strenuous activity before the test.
2
During the Test:The test involves drinking a glucose solution and having blood drawn multiple times over 5 hours.
3
After the Test:Resume normal diet and activities; results are typically available within 24-48 hours.

About This Test

Who Should Get This Test

To assess insulin resistance and diagnose diabetes by measuring blood glucose and insulin levels over 5 hours after glucose ingestion.

How to Prepare

  • Fast overnight (8-12 hours)
  • Dissolve 82.5g glucose monohydrate in 300mL water for adults
  • Administer glucose solution orally over 5 minutes
  • Draw blood samples at specified intervals
  • Label each container with time drawn and tests required

Doctor's Notes

Reviewed by — MBBS, MD (Biochemistry) · Reg. No. 21504

"This test is valuable for assessing beta cell function and insulin sensitivity in patients with suspected diabetes or insulin resistance."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePlasma from Grey Top tube and Serum from SST
Sample Volume2 mL per tube (1 mL minimum)
ContainerGrey Top (Sodium Fluoride) tube and SST
Collection MethodVenipuncture

Sample Stability

Room Temperature: 2 hours
Refrigerator: 24 hours
Frozen: 1 week
Sample Rejection Criteria:
  • Insufficient sample volume
  • Improper labeling
  • Sample not refrigerated or frozen as required

Understanding Your Results

Results from the Insulin Response to Glucose 5 Hours Test are interpreted by comparing blood glucose and insulin levels at various time points to standard reference ranges.
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Glucose and insulin levels return to normal within 5 hours, indicating proper insulin sensitivity.

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Elevated insulin levels with high glucose levels suggest the body is not responding effectively to insulin.

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Persistently high glucose levels indicate impaired glucose metabolism and possible diabetes.

⚠️ When to Consult a Doctor:

Consult a doctor if you experience symptoms of diabetes, have abnormal test results, or have risk factors such as obesity or family history.

Limitations

  • Results may vary based on individual physiology
  • Not definitive for all types of diabetes
  • Requires multiple blood draws over 5 hours

Risks & Considerations

  • Minor bruising at blood draw site
  • Dizziness or nausea from glucose solution
  • Rare allergic reactions

Interfering Factors

  • Recent carbohydrate intake
  • Medications affecting glucose metabolism
  • Physical stress or illness
  • Smoking or alcohol consumption

Frequently Asked Questions

What is the Insulin Response to Glucose 5 Hours Test?
It is a diagnostic test that measures how your body responds to glucose by checking blood sugar and insulin levels over 5 hours after drinking a glucose solution.
Why is this test performed?
This test is performed to assess insulin resistance and diagnose diabetes, especially in individuals with symptoms like increased thirst, frequent urination, or unexplained weight loss.
How should I prepare for the test?
You must fast overnight (8-12 hours) before the test. Avoid eating or drinking anything except water during the fasting period.
What happens during the test?
You will drink a glucose solution, and blood samples will be drawn at multiple intervals (e.g., 0.5, 1, 1.5, 2, 2.5, 3, 4, and 5 hours) to measure glucose and insulin levels.
Are there any risks associated with the test?
Risks are minimal and may include bruising at the blood draw site, dizziness, or nausea from the glucose solution. Serious reactions are rare.
How long does the test take?
The test takes approximately 5 hours to complete, including multiple blood draws at specified intervals.
What do the results indicate?
Results show how your body processes glucose and produces insulin. Abnormal levels may indicate insulin resistance or diabetes.
What is the cost of the test in India?
The cost is INR 10,500, which includes home sample collection in many cities across India.
Is home collection available for this test?
Yes, free home sample collection is available for online bookings in numerous cities including Mumbai, Delhi, Bangalore, and more.
How long does it take to get the results?
Results are typically available within 24-48 hours and can be accessed online, via email, or WhatsApp.
Can this test diagnose both type 1 and type 2 diabetes?
Yes, it helps assess insulin resistance and beta cell function, which can be relevant for diagnosing both types of diabetes.
What should I do if my results are abnormal?
Consult an endocrinologist or healthcare provider for further evaluation, diagnosis, and management of your condition.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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