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LH & Testosterone Total Test

DNA Labs India | ISO 9001:2015 Certified

LH & Testosterone Total Test

Short Name: LH & Testosterone

Also known as: Luteinizing Hormone & Testosterone Test, LH & Total Testosterone Panel, LH Testosterone Serum Test, Hormone Panel LH and Testosterone

LH & Testosterone Total Test test available at DNA Labs India for ₹1,322. Uses Chemiluminescent Immunoassay (CLIA) on Serum samples. Results in Reports are generally available within 24 hours of sample collection. You will be notified via SMS, email, or WhatsApp when your report is ready for download.. Free home collection in 300+ cities across India.

EndocrinologistAdults🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Ramarao Paidisetty

Dr Ramarao Paidisetty

Consultant Biochemist · Reg: 21504

Last reviewed: September 7, 2026

Overview

This test is ordered to evaluate hormonal balance related to reproductive health and endocrine function. It helps diagnose conditions such as hypogonadism, infertility, pituitary gland disorders, polycystic ovary syndrome (PCOS), and other disorders of the endocrine system. It is also used to monitor hormone replacement therapy and assess testicular or ovarian function.

Test Code
1173
Price
₹1,322
Sample Type
Serum
Result Time
Reports are generally available within 24 hours of sample collection. You will be notified via SMS, email, or WhatsApp when your report is ready for download.
Fasting Required
No
Method
Chemiluminescent Immunoassay (CLIA)
Step 1

Sample Collection

No fasting is required. Inform your doctor about any medications, supplements, or hormone therapies you are currently taking. For female patients, specify the day of the menstrual cycle on the test request form; Day 2 or Day 3 is preferred for baseline LH assessment.

Method: Venipuncture

Step 2

Laboratory Analysis

A standard venipuncture blood draw will be performed. A tourniquet is applied to the upper arm, and approximately 3 mL of blood is drawn from a vein into a Serum Separator Tube (SST). The procedure typically takes less than 5 minutes.

Step 3

Report Delivery

Apply gentle pressure to the puncture site with a cotton ball or bandage for 2–3 minutes. Mild bruising may occur and usually resolves within a few days. You may resume normal activities immediately after the blood draw.

Timeline: Reports are generally available within 24 hours of sample collection. You will be notified via SMS, email, or WhatsApp when your report is ready for download.

Patient Instructions

1
Before the Test:No special preparation is required. No fasting is needed. Avoid testosterone supplements or hormonal medications for at least 48 hours before the test unless otherwise directed by your physician. For female patients, schedule the blood draw on Day 2 or Day 3 of the menstrual cycle for baseline LH evaluation.
2
During the Test:A healthcare professional will draw a small blood sample (approximately 3 mL) from a vein in your arm using a needle and syringe or vacuum tube system. The process takes about 5 minutes and causes minimal discomfort.
3
After the Test:After the blood draw, a small bandage will be placed over the puncture site. You may experience slight bruising or soreness at the site. Results are typically available the same day or next day via online portal, email, or WhatsApp.

About This Test

Who Should Get This Test

This test is ordered to evaluate hormonal balance related to reproductive health and endocrine function. It helps diagnose conditions such as hypogonadism, infertility, pituitary gland disorders, polycystic ovary syndrome (PCOS), and other disorders of the endocrine system. It is also used to monitor hormone replacement therapy and assess testicular or ovarian function.

How to Prepare

  • No fasting required before sample collection
  • For females, preferred sampling time is Day 2 or Day 3 of the menstrual cycle
  • Specify age, sex, and day of menstrual cycle on the test request form
  • Avoid testosterone supplements or anabolic steroids for at least 48 hours before the test unless directed otherwise by your physician
  • Morning blood draw is recommended for accurate testosterone assessment
  • Sample: 3 mL (1 mL min.) serum from 1 SST. Ship refrigerated or frozen.

Doctor's Notes

Reviewed by — MBBS, MD (Biochemistry) · Reg. No. 21504

"LH and total testosterone levels are fundamental for evaluating reproductive and endocrine health in both men and women. In males, these tests are essential for diagnosing hypogonadism, evaluating infertility, and monitoring testosterone replacement therapy. In females, LH assessment is critical for understanding ovulatory function and menstrual irregularities. I recommend this panel for any patient presenting with symptoms such as low libido, erectile dysfunction, fatigue, unexplained weight changes, or irregular menstrual cycles. Early detection of hormonal imbalances allows for timely intervention and significantly improves patient outcomes."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume3 mL (1 mL min.) serum from 1 SST
ContainerSerum Separator Tube (SST)
Collection MethodVenipuncture

Sample Stability

Room Temperature: 8 hours
Refrigerated (2–8°C): 48 hours
Frozen (≤ -20°C): 4 weeks
Sample Rejection Criteria:
  • Hemolyzed, lipemic, or icteric samples may interfere with results
  • Sample collected in incorrect tube type
  • Insufficient sample volume (less than 1 mL serum)
  • Sample exceeding stability limits
  • Missing patient information (age, sex, menstrual cycle day)

Understanding Your Results

The LH & Testosterone Total Test results should be interpreted by a qualified endocrinologist or physician based on the patient's age, sex, clinical symptoms, and medical history. Both low and elevated levels can indicate different underlying conditions.
📊

Elevated LH with low testosterone suggests the testes are not functioning properly despite adequate pituitary stimulation. Causes include Klinefelter syndrome, testicular injury, or orchitis.

📊

Low or inappropriately normal LH with low testosterone indicates a pituitary or hypothalamic cause such as pituitary tumors, hyperprolactinemia, or hypothalamic dysfunction.

📊

Both values within normal limits suggest adequate endocrine function. Clinical correlation with symptoms is still recommended.

📊

An elevated LH to FSH ratio with raised total testosterone is characteristic of PCOS and may present with irregular periods, acne, hirsutism, and infertility.

📊

Elevated LH with low estrogen and variable testosterone may indicate premature ovarian insufficiency or menopause.

⚠️ When to Consult a Doctor:

Consult an endocrinologist or your physician if your test results are outside the normal range, or if you experience persistent symptoms such as low sex drive, erectile dysfunction, infertility, irregular periods, unexplained fatigue, or loss of muscle mass. Prompt evaluation can help identify the underlying cause and guide appropriate treatment.

Limitations

  • ⚠Total testosterone includes both protein-bound and free fractions and may not reflect bioavailable testosterone
  • ⚠Single measurement may not capture diurnal variation; morning sampling is preferred for testosterone
  • ⚠Results should always be interpreted in clinical context alongside patient history and physical examination
  • ⚠Borderline values may require repeat testing for confirmation

Risks & Considerations

  • ●Minor bruising or discomfort at the venipuncture site
  • ●Rare risk of infection at the puncture site
  • ●Lightheadedness or fainting in individuals sensitive to blood draws

Interfering Factors

  • ●Recent use of testosterone supplements or anabolic steroids
  • ●Acute illness or severe stress may temporarily alter hormone levels
  • ●Hormonal medications including oral contraceptives, GnRH agonists, or anti-androgens
  • ●Time of day – testosterone levels are highest in the morning
  • ●In females, incorrect timing within the menstrual cycle may affect LH levels
  • ●Obesity may lower total testosterone levels

Compare With Similar Tests

TestLH & Testosterone Total TestFree Testosterone TestFSH (Follicle Stimulating Hormone)SHBG (Sex Hormone Binding Globulin)
ComparisonLH & Testosterone Total Test

Frequently Asked Questions

What is the LH & Testosterone Total Test?
The LH & Testosterone Total Test is a blood test that measures the levels of Luteinizing Hormone (LH) and Total Testosterone in your blood. LH is produced by the pituitary gland and stimulates testosterone production in males and ovulation in females. Testosterone is essential for male sexual health, muscle mass, bone density, and overall well-being.
Why would a doctor order the LH & Testosterone Total Test?
A doctor may order this test if you are experiencing symptoms such as low sex drive, erectile dysfunction, infertility, irregular menstrual cycles, fatigue, loss of muscle mass, or depression. It helps diagnose conditions like hypogonadism, pituitary disorders, PCOS, and other endocrine abnormalities.
What is the cost of the LH & Testosterone Total Test at DNA Labs India?
The cost of the LH & Testosterone Total Test at DNA Labs India is Rs 1322.1. This includes free home sample collection across 300+ cities in India and digital report delivery via online portal, email, and WhatsApp.
Do I need to fast before the LH & Testosterone Total Test?
No, fasting is not required for the LH & Testosterone Total Test. However, for female patients, the preferred sampling time is Day 2 or Day 3 of the menstrual cycle for accurate baseline LH evaluation. It is also recommended to have your blood drawn in the morning when testosterone levels are typically highest.
What sample is required for the LH & Testosterone Total Test?
The test requires 3 mL (1 mL minimum) of serum collected from a blood draw into a Serum Separator Tube (SST). The sample should be shipped refrigerated or frozen to maintain stability.
What are normal LH and testosterone levels?
Normal LH levels for adult males typically range from 1.5 to 9.3 mIU/mL. For females, normal ranges vary by menstrual cycle phase (follicular: 2.4–12.6 mIU/mL, midcycle peak: 14.0–95.6 mIU/mL, luteal: 1.0–11.4 mIU/mL). Normal total testosterone for adult males is 240–950 ng/dL and for adult females is 8–60 ng/dL. Your doctor will interpret your results based on your age, sex, and clinical context.
What does it mean if my LH is high but testosterone is low?
Elevated LH with low testosterone typically suggests primary hypogonadism, meaning the testes are not producing sufficient testosterone despite adequate stimulation from the pituitary gland. This may be due to conditions such as Klinefelter syndrome, testicular injury, or infection. Your endocrinologist will recommend further evaluation and treatment.
What does it mean if both LH and testosterone are low?
Low LH with low testosterone may indicate secondary hypogonadism, where the problem originates from the pituitary gland or hypothalamus rather than the testes. Causes include pituitary tumors, hyperprolactinemia, or certain medications. A physician will typically order additional tests such as an MRI of the pituitary gland and prolactin levels.
Is the LH & Testosterone Total Test available with home sample collection?
Yes, DNA Labs India offers free home sample collection for the LH & Testosterone Total Test in over 300 cities across India, including Mumbai, Delhi, Bangalore, Hyderabad, Chennai, Kolkata, Pune, Ahmedabad, Jaipur, Lucknow, and many more. You can book online for convenient home collection.
How long does it take to get the results of the LH & Testosterone Total Test?
Reports for the LH & Testosterone Total Test are typically available the same day or next day after sample collection. You will receive your results via the DNA Labs India online portal, email, or WhatsApp.
Can women take the LH & Testosterone Total Test?
Yes, this test is relevant for both men and women. In women, LH levels are critical for evaluating ovulatory function, diagnosing PCOS, and understanding menstrual irregularities. Total testosterone levels in women can help identify conditions like PCOS or adrenal disorders. Female patients should specify the day of their menstrual cycle on the test request form, with Day 2 or Day 3 being preferred.
What method is used for the LH & Testosterone Total Test?
The LH & Testosterone Total Test at DNA Labs India uses the Chemiluminescent Immunoassay (CLIA) method. CLIA is a highly sensitive and specific laboratory technique that provides accurate and reliable measurements of hormone levels in blood serum.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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