NLGN4X Gene Autism Susceptibility, X-Linked Type 2 NGS Genetic Test
Short Name: NLGN4X Autism NGS
Also known as: NLGN4X Gene Sequencing, Autism X-Linked Type 2 Genetic Test, Neuroligin 4X Gene Test
NLGN4X Gene Autism Susceptibility, X-Linked Type 2 NGS Genetic Test test available at DNA Labs India for ₹20,000. Uses Next-Generation Sequencing (NGS) on Blood or Extracted DNA or One drop Blood on FTA Card samples. Results in The turnaround time is 3 to 4 weeks from the date the sample is received at the laboratory. The report will include the NGS findings, variant interpretation, and clinical summary.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of this NGS genetic test is to identify disease-causing mutations in the NLGN4X gene that increase susceptibility to autism spectrum disorder. The test helps in confirming a genetic diagnosis, enabling early intervention, personalized management, and informed genetic counselling for the patient and family members.
- Test Code
- 3904
- Price
- ₹20,000
- Sample Type
- Blood or Extracted DNA or One drop Blood on FTA Card
- Result Time
- The turnaround time is 3 to 4 weeks from the date the sample is received at the laboratory. The report will include the NGS findings, variant interpretation, and clinical summary.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS)
Sample Collection
No fasting required. A genetic counselling session is recommended to draw a pedigree chart of family members affected with NLGN4X Gene Autism susceptibility. Please bring any prior genetic testing reports, clinical records, and a detailed family history.
Method: Peripheral blood draw, saliva collection or FTA card spot
Laboratory Analysis
A trained phlebotomist will collect 2-3 mL venous blood into an EDTA tube for DNA extraction. Alternatively, a saliva sample may be collected using an Oragene kit, or a few drops of blood can be placed on an FTA card. The procedure is quick and safe.
Report Delivery
The sample is labeled and transported to the DNA Labs India laboratory at ambient temperature. The patient can resume normal activities immediately. The sample will be processed within a few days, and a report will be delivered in 3 to 4 weeks.
Timeline: The turnaround time is 3 to 4 weeks from the date the sample is received at the laboratory. The report will include the NGS findings, variant interpretation, and clinical summary.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of this NGS genetic test is to identify disease-causing mutations in the NLGN4X gene that increase susceptibility to autism spectrum disorder. The test helps in confirming a genetic diagnosis, enabling early intervention, personalized management, and informed genetic counselling for the patient and family members.
How to Prepare
- Do not eat or drink anything for 30 minutes before saliva collection if using a saliva kit
- Do not use mouthwash before saliva collection
- If using blood sample, no special preparations are needed
- Ensure the sample container is labeled correctly
- Transport the sample at room temperature if the arrival at lab is within 24 hours; refrigerate if storing for up to 7 days
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"Genetic testing for NLGN4X is recommended for families with suspected X-linked autism or related neurodevelopmental conditions. Genetic counselling should accompany testing to help interpret results and guide reproductive decisions."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or clotted blood sample
- Insufficient sample volume
- Sample leaking from container
- Mislabeled or unlabeled sample
- Sample received after prolonged delay without proper storage
Understanding Your Results
No pathogenic variant detected
The NLGN4X gene shows no known disease-causing variants; a genetic cause is not found in this gene, but autism may still be present due to other genes or environmental factors.
Pathogenic / Likely pathogenic variant detected
A disease-associated variant is present, confirming the genetic susceptibility. Genetic counselling is recommended for family testing and risk assessment.
Variant of uncertain significance (VUS)
A genetic variant was found, but its association with autism is not yet clear. Additional family studies or functional analysis may be needed.
Benign/Likely benign variant
The identified variant has no known disease association; it is considered a normal genetic variation.
Consult your healthcare provider if you or your child show signs of autism spectrum disorder, including persistent communication difficulties, lack of eye contact, repetitive behaviors, or delayed language skills. A specialist can help arrange developmental assessment and genetic testing.
Limitations
- ⚠This test only analyzes the NLGN4X gene and is not used to diagnose all types of autism
- ⚠Variants of uncertain clinical significance (VUS) may be reported and require further interpretation
- ⚠Large deletions, duplications, or chromosomal rearrangements are not reliably detected by standard NGS sequencing
- ⚠The test does not assess other genetic causes of autism, such as SHANK3, MECP2, or FMR1 mutations
- ⚠Negative results do not completely exclude a genetic basis of autism
Risks & Considerations
- ●No significant physical risks are associated with blood collection; minor bruising or discomfort may occur
- ●Psychological implications of a positive result, including anxiety or future reproductive planning concerns
- ●Possibility of identifying variants of uncertain significance, occasionally leading to further testing
- ●Privacy and confidentiality considerations related to genetic information
Interfering Factors
- ●Contamination of sample with foreign DNA
- ●Degraded or low-quality DNA
- ●Maternal cell contamination in prenatal samples
- ●Rare sequencing errors in low-complexity regions
- ●Incomplete gene coverage due to variants near the sequencing primers
Compare With Similar Tests
| Test | NLGN4X Gene Autism Susceptibility, X-Linked Type 2 NGS Genetic Test | NLGN4X Gene NGS Test | MECP2 Gene Rett Syndrome Test | FMR1 Gene Fragile X Test | Autism Comprehensive NGS Panel |
|---|---|---|---|---|---|
| Comparison | NLGN4X Gene Autism Susceptibility, X-Linked Type 2 NGS Genetic Test |
Frequently Asked Questions
What is the NLGN4X gene?
Who is this test meant for?
What does the NGS technique detect in this test?
What sample is required?
Is fasting required before the test?
How long does it take to get the report?
Why should I ask for raw data, FASTQ, and VCF files?
Are there any risks in this test?
Can this test diagnose autism?
What if the test result is 'negative'?
Does DNA Labs India provide home sample collection?
What is the cost of the test?
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