Parvovirus B19 Qualitative PCR Test
Short Name: B19V PCR
Also known as: Parvovirus B19 DNA PCR, B19V Qualitative PCR, Human Parvovirus B19 PCR
Parvovirus B19 Qualitative PCR Test test available at DNA Labs India for ₹5,000. Uses Real Time PCR on Whole Blood (EDTA) or CSF samples. Results in Samples received by Monday/Thursday before 11 am are reported by Wednesday/Saturday via online portal, email, or WhatsApp.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of this qualitative PCR test is to confirm the presence or absence of Parvovirus B19 DNA in a clinical specimen. PCR is especially useful in early infection before antibodies appear, in immunocompromised patients who may not mount an antibody response, and in pregnancy when a rapid, sensitive diagnosis is needed to guide monitoring and treatment. Because the test is qualitative, it reports whether the virus is detected or not detected rather than providing a viral count.
- Test Code
- 3540
- Price
- ₹5,000
- Sample Type
- Whole Blood (EDTA) or CSF
- Result Time
- Samples received by Monday/Thursday before 11 am are reported by Wednesday/Saturday via online portal, email, or WhatsApp.
- Fasting Required
- No
- Method
- Real Time PCR
Sample Collection
No special preparation or fasting is required.
Method: Blood draw; CSF collection by a healthcare professional
Laboratory Analysis
A small blood sample will be collected from a vein. If CSF is needed, a doctor or trained healthcare professional will perform a lumbar puncture.
Report Delivery
You can resume normal activities after blood collection. If a lumbar puncture was performed, follow the treating doctor's instructions.
Timeline: Samples received by Monday/Thursday before 11 am are reported by Wednesday/Saturday via online portal, email, or WhatsApp.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this qualitative PCR test is to confirm the presence or absence of Parvovirus B19 DNA in a clinical specimen. PCR is especially useful in early infection before antibodies appear, in immunocompromised patients who may not mount an antibody response, and in pregnancy when a rapid, sensitive diagnosis is needed to guide monitoring and treatment. Because the test is qualitative, it reports whether the virus is detected or not detected rather than providing a viral count.
How to Prepare
- No special preparation or fasting is required
- Submit 3 mL (2 mL minimum) whole blood in a Lavender Top (EDTA) tube
- If CSF is required, collect 3 mL (2 mL minimum) in a sterile screw-capped container
- Transport the sample refrigerated; do not freeze
- Label the sample properly with patient identification and request details
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"A positive Parvovirus B19 PCR result in pregnancy should trigger urgent obstetric evaluation, including fetal ultrasound and Doppler studies. In immunocompromised patients, persistent viremia may occur despite negative IgM antibodies, so PCR is often the preferred diagnostic test."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Frozen specimen
- Hemolyzed, clotted, or insufficient sample
- Blood sample received in a plain red-top tube instead of EDTA tube
- Unlabeled or mislabeled specimen
- Sample not transported within the acceptable stability period
Understanding Your Results
Not detected (Negative)
No Parvovirus B19 DNA was detected in the specimen. An active parvovirus B19 infection is unlikely, although false-negative results can occur if the sample was collected very early, very late, or handled incorrectly.
Detected (Positive)
Parvovirus B19 DNA was detected in the specimen. This indicates active or recent viral replication. The result must be interpreted in the context of symptoms, immune status, pregnancy status, and serology findings.
If you are pregnant and may have been exposed to parvovirus B19, if you have a chronic red blood cell disorder and develop sudden anemia, if you are immunocompromised and have persistent fever or anemia, or if your test result is positive, consult a doctor promptly.
Limitations
- ⚠Qualitative PCR does not provide a viral load or quantify the amount of virus
- ⚠Detection of B19 DNA does not by itself distinguish acute, recent, or persistent infection
- ⚠False-negative results are possible if the sample is not collected, transported, or stored according to specifications
- ⚠This test cannot determine past immunity or immune status
Risks & Considerations
- ●Minor bruising or discomfort at the blood collection site
- ●Rarely, bleeding, infection, or dizziness after blood draw
- ●Lumbar puncture has additional risks and will be explained by the treating physician
Interfering Factors
- ●Specimen collected very early or very late after infection may have viral DNA below the limit of detection
- ●Recent blood transfusion may affect interpretation
- ●Contamination during collection or processing can cause false-positive results
- ●Immunosuppression can alter viral clearance and antibody response
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