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Prothrombin Time Studies Test

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Prothrombin Time Studies Test

Short Name: Prothrombin Gene Mutation Analysis

Also known as: Prothrombin G20210A Mutation Analysis, Factor II Mutation Analysis, PT Gene Mutation Test

Prothrombin Time Studies Test test available at DNA Labs India for ₹4,500. Uses Real Time PCR on Whole Blood samples. Results in Samples received by Monday/Thursday by 11 AM are reported on Wednesday/Saturday, respectively. Reports will be available on the patient portal, via email, and on WhatsApp.. Free home collection in 300+ cities across India.

Real Time PCR🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SHAILAJA RAGHUNATH MURDESHWAR

Dr SHAILAJA RAGHUNATH MURDESHWAR

Consultant Physician · Reg: 8052

Last reviewed: September 7, 2026

Overview

To detect the prothrombin G20210A mutation, which increases the risk of venous thromboembolism (VTE) and helps guide preventive treatment decisions in at-risk individuals.

Test Code
3584
Price
₹4,500
Sample Type
Whole Blood
Result Time
Samples received by Monday/Thursday by 11 AM are reported on Wednesday/Saturday, respectively. Reports will be available on the patient portal, via email, and on WhatsApp.
Fasting Required
No
Method
Real Time PCR
Step 1

Sample Collection

No fasting is required. Please provide the duly filled Genomics Clinical Information Requisition Form (Form 20), which is mandatory. Inform the laboratory if you are taking anticoagulant therapy or are pregnant, as it may affect clinical correlation.

Method: Peripheral venipuncture

Step 2

Laboratory Analysis

A standard venipuncture will be performed to collect approximately 3 mL of whole blood into a lavender top (EDTA) tube. A tourniquet will be applied briefly to locate a suitable vein.

Step 3

Report Delivery

You may resume normal activities immediately. Apply gentle pressure to the puncture site for a few minutes to prevent bruising. There are no post-test restrictions.

Timeline: Samples received by Monday/Thursday by 11 AM are reported on Wednesday/Saturday, respectively. Reports will be available on the patient portal, via email, and on WhatsApp.

Patient Instructions

1
Before the Test:No special preparation is required. You should inform your healthcare provider about all medications you are taking, including over-the-counter drugs, herbal supplements, and hormonal therapies, as they may influence the clinical interpretation.
2
During the Test:A blood sample will be drawn from a vein in your arm. This procedure is quick and usually takes less than five minutes. You may experience mild discomfort during needle insertion.
3
After the Test:You can leave immediately after the blood draw. You may continue all regular activities, including exercising, as there are no restrictions. Any minor bruising at the puncture site will heal on its own.

About This Test

Who Should Get This Test

To detect the prothrombin G20210A mutation, which increases the risk of venous thromboembolism (VTE) and helps guide preventive treatment decisions in at-risk individuals.

How to Prepare

  • No fasting is required.
  • A duly filled Genomics Clinical Information Requisition Form (Form 20) is mandatory.
  • Please inform the laboratory if you are on blood thinners or have a known bleeding disorder.

Doctor's Notes

Reviewed by — MBBS, MD (General Medicine) · Reg. No. 8052

"Testing for prothrombin G20210A mutation is particularly useful in women with a history of recurrent miscarriages or venous thromboembolism in pregnancy. Identifying the mutation allows for tailored thromboprophylaxis and safer contraceptive choices."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeWhole Blood
Sample Volume3 mL (1.5 mL min.)
ContainerLavender Top (EDTA) tube
Collection MethodPeripheral venipuncture

Sample Stability

Room Temperature
Refrigerator
Frozen
Sample Rejection Criteria:
  • Hemolyzed sample
  • Clotted sample
  • Improper labeling or missing patient identification
  • Missing or incomplete Genomics Clinical Information Requisition Form
  • Sample transported without refrigeration or frozen shipment

Understanding Your Results

The prothrombin G20210A mutation is the second most common inherited predisposition to hypercoagulability. Heterozygous carriers have a 2 to 6 fold increased lifetime relative risk of venous thromboembolism. This risk is further elevated in combination with pregnancy, oral contraceptive use, surgery, or immobility.
📊

Negative (GG genotype)

No prothrombin G20210A mutation detected. The individual does not have an increased risk due to this specific genetic variant. Further evaluation for other thrombophilia may be considered if clinically indicated.

📊

Heterozygous (GA genotype)

One copy of the prothrombin G20210A mutation is present. This is associated with a 2 to 6 fold increased risk of venous thromboembolism. Prophylactic measures may be recommended in high-risk situations such as pregnancy, oral contraceptive use, or surgery.

📊

Homozygous (AA genotype)

Two copies of the prothrombin G20210A mutation are present. This is rare and is associated with a significantly higher risk of VTE. Ongoing medical follow-up and aggressive prophylaxis in high-risk situations are strongly advised.

⚠️ When to Consult a Doctor:

If you have a personal or family history of unprovoked blood clots, deep vein thrombosis, pulmonary embolism, unexplained recurrent miscarriages, or if you are considering hormonal contraceptives and have a known family history of thrombophilia, consult your doctor or a specialist to determine whether prothrombin gene mutation testing is appropriate.

Limitations

  • ⚠This test specifically detects the prothrombin G20210A mutation only and does not rule out other inherited or acquired thrombophilic conditions.
  • ⚠Results should be interpreted in the context of clinical, family and laboratory history.
  • ⚠The test does not measure functional prothrombin levels; it is a genetic susceptibility marker.

Risks & Considerations

  • ●Bruising or mild swelling at the venipuncture site
  • ●Infection at the needle site (rare)
  • ●Excessive bleeding (unlikely, but possible in individuals with bleeding disorders)

Interfering Factors

  • ●Sample contamination with foreign DNA
  • ●DNA degradation due to improper storage or transport
  • ●PCR inhibitors in the sample (e.g., heparin, EDTA excess)

Compare With Similar Tests

TestProthrombin Time Studies TestFactor V Leiden Mutation AnalysisD-Dimer TestProtein C / Protein S / Antithrombin Assays
ComparisonProthrombin Time Studies Test

Frequently Asked Questions

What is prothrombin gene mutation analysis?
Prothrombin gene mutation analysis is a simple blood test that identifies a specific genetic mutation in the prothrombin gene known as the G20210A mutation. This mutation increases the risk of developing blood clots, which can lead to deep vein thrombosis, pulmonary embolism, and stroke.
What is the prothrombin G20210A mutation?
The prothrombin G20210A mutation is a change in the F2 gene, which codes for prothrombin (factor II). This variant is associated with elevated prothrombin levels, leading to a 2 to 6 fold increased risk of venous thromboembolism in heterozygous carriers.
Who should get this test?
The test is recommended for individuals with a personal or family history of blood clots, recurrent miscarriages, unprovoked venous thromboembolism at a young age, or a known family history of this mutation. It may also be considered before using oral contraceptives or hormone replacement therapy in high-risk individuals.
Is fasting required for the prothrombin gene mutation test?
No, fasting is not required. However, it is mandatory to submit a duly filled Genomics Clinical Information Requisition Form (Form 20) along with the sample.
What type of sample is needed?
A 3 mL (1.5 mL minimum) whole blood sample in a lavender top (EDTA) tube is needed. The sample should be shipped refrigerated and must not be frozen.
How is the prothrombin gene mutation analysis performed?
The test is performed by Real Time PCR (Polymerase Chain Reaction). DNA is extracted from your white blood cells, and the specific region of the prothrombin gene is amplified and analyzed for the presence of the G20210A mutation.
What does a positive result mean?
A positive result means that the prothrombin G20210A mutation is present. The outcome is reported as either heterozygous or homozygous. This indicates an inherited increased risk for developing abnormal blood clots, and you should discuss preventive measures with your doctor.
Can this mutation cause recurrent miscarriages?
Yes, the prothrombin gene mutation can lead to placental blood clots, which may cause recurrent miscarriages. Testing is often recommended for women with unexplained pregnancy loss.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection for online bookings for this test. The service is available in multiple cities across India, including Mumbai, Delhi, Bangalore, Hyderabad, and many others.
When will I receive my test report?
Samples received by Monday or Thursday by 11 am are processed and reports are delivered on Wednesday or Saturday respectively. You can access your report online via the patient portal, email, or WhatsApp.
How much does the prothrombin gene mutation analysis test cost in India?
The special price of the test at DNA Labs India is INR 4500. The cost may vary based on location and additional tests if recommended by your doctor.
Is this test covered by insurance?
Insurance coverage depends on your individual policy. DNA Labs India provides a free home collection service for online bookings and offers the test at a discounted rate of Rs 4500 across India. We recommend checking with your insurance provider for reimbursement eligibility.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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