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DNA Labs India

Steroid Panel 2 9 Steroids Test

DNA Labs India | ISO 9001:2015 Certified

Steroid Panel 2 9 Steroids Test

Short Name: Steroid Panel 2 (9 Steroids)

Also known as: Steroid Panel 2, 9 Steroids Test, Anabolic Steroid Screening, Adrenal Steroid Panel

Steroid Panel 2 9 Steroids Test test available at DNA Labs India for ₹5,000. Uses LC-MS/MS on Serum samples. Results in Samples received by 9 am on Monday or Thursday will be reported by Wednesday or Saturday respectively; typical TAT is 2-3 days.. Free home collection in 300+ cities across India.

LC-MS/MSUnisexAll Ages🏠 Home Collection

🩺 Medically Reviewed By

Overview

This test assists in evaluating the steroidogenic biosynthetic pathway by measuring nine steroids simultaneously. It is used to diagnose adrenal disorders such as congenital adrenal hyperplasia, and to detect or monitor anabolic steroid use. The results help endocrinologists and other clinicians assess hormonal imbalances and plan appropriate treatment.

Test Code
3669
Price
₹5,000
Sample Type
Serum
Result Time
Samples received by 9 am on Monday or Thursday will be reported by Wednesday or Saturday respectively; typical TAT is 2-3 days.
Fasting Required
No
Method
LC-MS/MS
Step 1

Sample Collection

Sample should be collected between 8-10 am or 4-6 pm due to diurnal variation. No fasting is required. Please inform your doctor about any steroid or hormonal medications you are taking.

Method: Venipuncture

Step 2

Laboratory Analysis

A standard venipuncture will be performed. The procedure is safe and typically takes less than 5 minutes.

Step 3

Report Delivery

No special precautions are necessary after the blood draw. You can resume normal activities immediately.

Timeline: Samples received by 9 am on Monday or Thursday will be reported by Wednesday or Saturday respectively; typical TAT is 2-3 days.

Patient Instructions

1
Before the Test:Inform the lab team about all prescribed and over-the-counter medicines, supplements, or anabolic steroids you are taking. Schedule your sample between 8-10 am or 4-6 pm.
2
During the Test:A tourniquet is applied, blood is drawn from the arm vein into a plain red-top tube. The procedure is quick and usually painless.
3
After the Test:A small bandage will be placed over the puncture site. You may remove it after an hour. Report will be available as per the scheduled turnaround time.

About This Test

Who Should Get This Test

This test assists in evaluating the steroidogenic biosynthetic pathway by measuring nine steroids simultaneously. It is used to diagnose adrenal disorders such as congenital adrenal hyperplasia, and to detect or monitor anabolic steroid use. The results help endocrinologists and other clinicians assess hormonal imbalances and plan appropriate treatment.

How to Prepare

  • Bring a valid ID and lab requisition form.
  • Avoid taking any non-prescribed steroid supplements 24 hours prior to the test.
  • Specify the collection time and date on the test request form.
  • Sample to be collected in a Red Top (No Additive) tube; do not use SST gel barrier tubes.

Doctor's Notes

Reviewed by — MBBS, MD (Biochemistry) · Reg. No. 21504

"This panel offers a comprehensive assessment of the steroidogenic pathway, allowing accurate diagnosis of adrenal enzymatic disorders and anabolic steroid abuse. The LC-MS/MS methodology provides highly specific quantification, aiding in precise clinical decision-making."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL (1 mL min.)
ContainerRed Top (No Additive) tube; Do not use SST gel barrier tubes
Collection MethodVenipuncture

Sample Stability

Room Temperature: 8 hours
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 7 weeks
Sample Rejection Criteria:
  • Samples collected in SST gel barrier tubes
  • Haemolysed or lipemic samples
  • Samples with incorrect labeling or missing collection time
  • Samples stored improperly or beyond stability period

Understanding Your Results

Interpretation of the Steroid Panel 2 should be performed by a qualified endocrinologist or clinical biochemist. Elevated or decreased levels of these steroids can indicate specific enzyme defects, adrenal dysfunction, or exogenous steroid use.
Elevated 17-α-Hydroxyprogesterone and androstenedione suggest 21-hydroxylase deficiency (classic CAH).
Elevated DHEAS and testosterone may indicate adrenal or ovarian hyperandrogenism.
Suppressed testosterone and LH/FSH with detectable nandrolone or stanozolol indicate anabolic steroid use.
Low cortisol and abnormal steroid ratios may point to adrenal insufficiency.
Abnormal progesterone and estradiol levels can be present in gonadal disorders.
⚠️ When to Consult a Doctor:

Please consult an endocrinologist or the referring physician if your results are outside the reference range or if you experience symptoms such as unexplained virilization, hirsutism, menstrual irregularities, or suspected steroid abuse.

Limitations

  • The panel may not detect all possible synthetic steroids or designer drugs
  • Reference ranges are age- and gender-specific and may vary by laboratory
  • A single measurement may not capture cyclical variations
  • Results should be interpreted in the context of clinical presentation and other tests

Risks & Considerations

  • Slight discomfort or bruising at the needle site
  • Dizziness or fainting in rare cases
  • Infection (very low risk with proper phlebotomy techniques)

Interfering Factors

  • Collection outside the recommended due to diurnal variation
  • Recent intake of steroid medications or supplements
  • Use of oral contraceptives or hormonal therapy
  • Sample contamination or improper storage
  • Haemolysis of the sample

Compare With Similar Tests

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Frequently Asked Questions

What is the Steroid Panel 2 - 9 Steroids test?
It is a comprehensive blood test that measures 9 different steroid hormones (androstenedione, DHEA, DHEAS, DHT, estradiol, 17-OHP, progesterone, testosterone, and 11-deoxycorticosterone) using LC-MS/MS. It helps evaluate adrenal and gonadal function and detect steroid abuse.
What steroids are detected in this panel?
This panel measures Testosterone, Dihydrotestosterone (DHT), Nandrolone, Stanozolol, Methandienone, Oxandrolone, Oxymetholone, Fluoxymesterone, and Trenbolone. In addition, it also measures endogenous steroids such as androstenedione, DHEA, DHEAS, estradiol, 17-OHP, progesterone, and 11-deoxycorticosterone.
What is the cost of this test at DNA Labs India?
The test costs INR 5000 at DNA Labs India. Free home sample collection is available for online bookings in most major cities across India.
Is this test used for steroid abuse detection?
Yes, the test is designed to detect the presence of nine common anabolic steroids. It is helpful in diagnosing suspected steroid abuse, especially in athletes or individuals with symptoms of anabolic steroid use.
Do I need to fast for this test?
No, fasting is not required. However, it is important to collect the sample at the recommended time (8-10 am or 4-6 pm) because steroid levels show diurnal variation.
When should I schedule my sample collection?
You should schedule your sample collection between 8-10 am or 4-6 pm, as instructed by your physician or the test requisition. The specific time of collection must be recorded on the sample container and test request form.
What is the sample type required?
The test requires 2 mL (minimum 1 mL) serum from a Red Top (No Additive) tube. Do not use SST gel barrier tubes as they may interfere with the LC-MS/MS analysis.
How long does it take to get results?
Samples received by 9 am on Monday or Thursday are processed and reports are available by Wednesday or Saturday respectively. Generally, the turnaround time is 2-3 days.
Can this test diagnose congenital adrenal hyperplasia (CAH)?
Yes, this panel is valuable in diagnosing CAH by measuring steroid precursors like 17-?-hydroxyprogesterone and androstenedione. Elevated levels can indicate enzyme deficiencies such as 21-hydroxylase deficiency.
Is home sample collection available?
Yes, DNA Labs India provides free home sample collection for online bookings of this test across Mumbai, Delhi, Bangalore, Hyderabad, Ahmedabad, Chennai, Kolkata, and more than 200 cities in India.
Which cities is this test available in?
The test is available in all major Indian cities including Mumbai, Delhi, Bangalore, Hyderabad, Ahmedabad, Chennai, Kolkata, Surat, Pune, Jaipur, Lucknow, Kanpur, Nagpur, Indore, and many others. Free home collection is provided at no extra cost.
What does the LC-MS/MS method mean?
LC-MS/MS stands for Liquid Chromatography-Tandem Mass Spectrometry. It is a highly sensitive and specific analytical technique used to accurately detect and quantify multiple steroids in a single sample. It is the gold standard for steroid measurement.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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