Steroid Panel: 21-Hydroxylase Deficiency Test
Short Name: 21-OHD Steroid Panel
Also known as: 21-Hydroxylase Deficiency Steroid Panel, Adrenal Steroid Profile, CAH Steroid Profile, 17-OHP + 21-Deoxycortisol Profile
Steroid Panel: 21-Hydroxylase Deficiency Test test available at DNA Labs India for ₹4,000. Uses LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) on Serum samples. Results in Samples received by 9 am on Monday generate reports on Wednesday; samples received by 9 am on Thursday generate reports on Saturday.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
The primary purpose of the Steroid Panel: 21-Hydroxylase Deficiency Test is to evaluate adrenal steroid hormone synthesis in individuals suspected of having 21-hydroxylase deficiency. It measures 17-hydroxyprogesterone, androstenedione, cortisol, and 21-deoxycortisol using LC-MS/MS, and provides a ratio to improve diagnostic interpretation. This panel is particularly useful in confirming cases of congenital adrenal hyperplasia (CAH), differentiating non-classical forms, and reducing false-positive newborn screening results.
- Test Code
- 3667
- ICD Code
- E25.0
- Price
- ₹4,000
- Sample Type
- Serum
- Result Time
- Samples received by 9 am on Monday generate reports on Wednesday; samples received by 9 am on Thursday generate reports on Saturday.
- Fasting Required
- No
- Method
- LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)
Sample Collection
No fasting is required. The sample should be collected between 8-10 am or 4-6 pm. Please write the exact collection date and time on the specimen container and test request form.
Method: Venous blood draw
Laboratory Analysis
A trained phlebotomist will draw approximately 2 mL of venous blood into a red top tube without additive. The procedure takes only a few minutes.
Report Delivery
You can resume your normal routine immediately. Keep the collection site clean and apply pressure if bleeding occurs.
Timeline: Samples received by 9 am on Monday generate reports on Wednesday; samples received by 9 am on Thursday generate reports on Saturday.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the Steroid Panel: 21-Hydroxylase Deficiency Test is to evaluate adrenal steroid hormone synthesis in individuals suspected of having 21-hydroxylase deficiency. It measures 17-hydroxyprogesterone, androstenedione, cortisol, and 21-deoxycortisol using LC-MS/MS, and provides a ratio to improve diagnostic interpretation. This panel is particularly useful in confirming cases of congenital adrenal hyperplasia (CAH), differentiating non-classical forms, and reducing false-positive newborn screening results.
How to Prepare
- Collect 2 mL serum in a red top tube with no additive
- Do not use SST gel barrier tubes
- Collect between 8-10 am or 4-6 pm and write the exact date and time on the container and request form
- Ship refrigerated or frozen
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"A single elevated 17-OHP value is not diagnostic. This steroid panel should be interpreted using age- and sex-specific reference intervals. If baseline results are inconclusive, an ACTH stimulation test or CYP21A2 genetic analysis may be required."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- SST gel barrier tube used
- Time of sample collection not provided
- Sample not collected during the specified diurnal window
- Improper labeling or incorrect transport temperature
Understanding Your Results
See an endocrinologist promptly if you or your child have symptoms such as ambiguous genitalia, unexplained vomiting, dehydration, low blood pressure, early puberty, severe hirsutism, irregular periods, infertility, or an abnormal steroid panel result.
Limitations
- ⚠This panel may not distinguish all genetic causes of CAH; CYP21A2 genetic testing may be required
- ⚠Borderline results may require ACTH stimulation testing for confirmation
- ⚠Reference intervals must be adjusted for age, sex, pubertal stage, and clinical context
Risks & Considerations
- ●Minimal pain or bruising at the puncture site
- ●Rarely, bleeding, infection, or dizziness after blood collection
Interfering Factors
- ●Physiological stress can cause false positive 17-OHP results in newborns
- ●Glucocorticoid or sex-steroid therapy may alter steroid hormone levels
- ●Sample collection outside the specified diurnal window may affect interpretation
Compare With Similar Tests
| Test | Steroid Panel: 21-Hydroxylase Deficiency Test | ||
|---|---|---|---|
| Comparison | Steroid Panel: 21-Hydroxylase Deficiency Test |
Frequently Asked Questions
What is the cost of the Steroid Panel: 21-Hydroxylase Deficiency Test at DNA Labs India?
What sample is needed for this test?
Is fasting required before the test?
Why are the collection times important?
Which hormones are measured in the steroid panel?
What is 21-hydroxylase deficiency?
What is congenital adrenal hyperplasia (CAH)?
How will my results be interpreted?
Can this test be used for newborn screening of CAH?
How long will the report take?
Is home sample collection available?
What should I do if my steroid panel is abnormal?
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