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DNA Labs India

Testosterone Free Test

DNA Labs India | ISO 9001:2015 Certified

Testosterone Free Test

Short Name: Free Testosterone

Also known as: Free Testosterone, Free T, Unbound Testosterone

Testosterone Free Test test available at DNA Labs India for ₹2,165. Uses Radioimmunoassay on Serum samples. Results in Samples submitted by 11 am from Monday to Saturday are processed on the same day, with reports available by evening.. Free home collection in 300+ cities across India.

Hormone TestAdult🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SHAILAJA RAGHUNATH MURDESHWAR

Dr SHAILAJA RAGHUNATH MURDESHWAR

Consultant Physician · Reg: 8052

Last reviewed: September 7, 2026

Overview

To determine the level of free testosterone in blood, evaluate androgen status, and assist in diagnosing conditions like hypogonadism.

Test Code
3683
CPT Code
84402
Price
₹2,165
Sample Type
Serum
Result Time
Samples submitted by 11 am from Monday to Saturday are processed on the same day, with reports available by evening.
Fasting Required
No
Method
Radioimmunoassay
Step 1

Sample Collection

No special preparation required. If possible, schedule the blood draw in the morning between 7 AM and 10 AM when testosterone levels are highest.

Method: Blood draw (venipuncture)

Step 2

Laboratory Analysis

A blood sample will be collected from a vein in your arm using a sterile needle. The procedure is quick and routinely performed.

Step 3

Report Delivery

You may resume your normal activities immediately. Keep pressure on the puncture site for a few minutes to prevent bruising.

Timeline: Samples submitted by 11 am from Monday to Saturday are processed on the same day, with reports available by evening.

Patient Instructions

1
Before the Test:No special preparation is necessary. It is best to schedule the test in the morning as testosterone levels follow a circadian rhythm. Inform your doctor about any medicines you are taking.
2
During the Test:A trained phlebotomist will draw approximately 3 ml of blood from a vein in your arm. The procedure takes only a few minutes.
3
After the Test:You can leave immediately and return to your routine. Rarely, minor bruising or soreness may occur at the puncture site.

About This Test

Who Should Get This Test

To determine the level of free testosterone in blood, evaluate androgen status, and assist in diagnosing conditions like hypogonadism.

How to Prepare

  • Provide complete details including age and sex on the test request form
  • No fasting is required
  • Stay well hydrated before sample collection
  • Inform your healthcare provider about any medications or supplements you are taking

Doctor's Notes

Reviewed by — MBBS, MD (General Medicine) · Reg. No. 8052

"Free testosterone is a crucial marker for androgen deficiency when total testosterone appears normal but symptoms persist."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume3 mL (1 mL min.)
Container1 SST (Serum Separator Tube)
Collection MethodBlood draw (venipuncture)

Sample Stability

Room Temperature: Not recommended
Refrigerated: 24 hours
Frozen: 4 weeks
Sample Rejection Criteria:
  • Haemolysed or lipemic samples
  • Samples received without age and sex information
  • Improperly labelled or transported samples

Understanding Your Results

The interpretation of free testosterone test results must be done by a medical professional. Values should be correlated with total testosterone, SHBG, and clinical symptoms.
Low free testosterone in men may indicate hypogonadism. Further evaluation with LH and FSH is often required to determine whether the cause is primary (testicular) or secondary (pituitary/hypothalamic).
High free testosterone in women may be seen in polycystic ovary syndrome (PCOS), congenital adrenal hyperplasia, or ovarian/adrenal tumours. Additional investigations may be recommended.
Borderline results should be interpreted alongside total testosterone, SHBG, and the patient's clinical picture before a diagnosis is made.
⚠️ When to Consult a Doctor:

Consult your doctor if you experience persistent symptoms such as fatigue, low libido, decreased muscle strength, or unexplained mood changes. Based on your symptoms and risk factors, your physician can decide whether the Testosterone Free Test is appropriate.

Limitations

  • ⚠Free testosterone levels vary throughout the day; a single measurement may not reflect the average level
  • ⚠The test alone cannot identify the underlying cause of abnormal results
  • ⚠Reference ranges may vary between laboratories and different assay methods

Risks & Considerations

  • ●Minor pain or bruising at the needle site
  • ●Dizziness or light-headedness during collection
  • ●Infection (very rare)

Interfering Factors

  • ●Circadian rhythm changes (testosterone peaks in the morning)
  • ●Use of biotin supplements up to 72 hours before testing
  • ●Certain medications, including corticosteroids and opioids
  • ●Recent illness, stress, or excessive alcohol consumption

Compare With Similar Tests

TestTestosterone Free Test
ComparisonTestosterone Free Test

Frequently Asked Questions

What is the Testosterone Free Test?
The testosterone free test is a blood test that measures the amount of unbound testosterone in your bloodstream. This is the testosterone not attached to proteins, which is free to circulate and act on tissues.
How much does the Testosterone Free Test cost at DNA Labs India?
At DNA Labs India, the discounted price for the Testosterone Free Test is INR 2164.5. This includes free home sample collection in many cities across India.
What is the difference between free testosterone and total testosterone?
Total testosterone measures all testosterone in the blood, including bound and free fractions. Free testosterone measures only the unbound, biologically active fraction, which is more readily used by the body.
Who should get a free testosterone test?
It is recommended for individuals experiencing symptoms of low testosterone, such as decreased libido, fatigue, depression, reduced muscle mass, or increased body fat. Your doctor may order it if clinical suspicion of hypogonadism exists.
Is fasting required for the Testosterone Free Test?
No, the Testosterone Free Test does not require fasting. No special preparation is needed.
What is the sample type for the free testosterone test?
The test requires 3 mL (1 mL minimum) of serum. This is collected using a serum separator tube (SST) from a routine blood draw.
When is the best time to take a free testosterone test?
Testing is usually performed in the morning when testosterone levels are at their peak. However, the test can be done at any time if your doctor advises otherwise.
How long does it take to get results?
If your sample is collected from Monday to Saturday by 11 am, reports are typically issued on the same day.
Is home sample collection available for the Testosterone Free Test?
Yes, DNA Labs India offers free home sample collection for online bookings in over 200 cities across India, including Mumbai, Delhi, Bangalore, Hyderabad, Chennai, and many more.
What does low free testosterone indicate?
Low free testosterone can suggest hypogonadism or androgen deficiency. It may be associated with fatigue, low libido, decreased muscle mass, and other symptoms. Your doctor may recommend further tests to identify the cause.
Can women take the Testosterone Free Test?
Yes, testosterone is present in both men and women. The test can be used to evaluate androgen status in women, especially in conditions like polycystic ovary syndrome (PCOS) and adrenal disorders.
How should I interpret the results?
Interpretation should be done by a qualified expert. High or low levels are compared with reference ranges based on age, sex, and the laboratory. Clinical correlation with symptoms and other tests is essential for diagnosis.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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