Vitamin D Ultrasensitive Test
Short Name: Vitamin D Ultrasensitive
Also known as: 25(OH)D Ultrasensitive Test, Vitamin D LC-MS/MS, 25-Hydroxy Vitamin D Test
Vitamin D Ultrasensitive Test test available at DNA Labs India for ₹3,000. Uses LC-MS/MS, 25-Hydroxy Vitamin D2/D3 Quantification on Serum samples. Results in Samples received by 11 am from Monday to Saturday are usually reported the same day.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
To accurately quantify 25-Hydroxy Vitamin D2 and D3 for diagnosing vitamin D deficiency, guiding replacement therapy, and evaluating disorders of bone and calcium metabolism.
- Test Code
- 3742
- CPT Code
- 82306
- ICD Code
- E55.9
- Price
- ₹3,000
- Sample Type
- Serum
- Result Time
- Samples received by 11 am from Monday to Saturday are usually reported the same day.
- Fasting Required
- No
- Method
- LC-MS/MS, 25-Hydroxy Vitamin D2/D3 Quantification
Sample Collection
No special preparation required. Inform your doctor about any supplements, especially biotin or vitamin D.
Method: Venous blood draw
Laboratory Analysis
A small blood sample is drawn from a vein in the arm. The process takes only a few minutes.
Report Delivery
You may resume normal activities immediately. Mild bruising or soreness at the puncture site may occur.
Timeline: Samples received by 11 am from Monday to Saturday are usually reported the same day.
Patient Instructions
About This Test
Who Should Get This Test
To accurately quantify 25-Hydroxy Vitamin D2 and D3 for diagnosing vitamin D deficiency, guiding replacement therapy, and evaluating disorders of bone and calcium metabolism.
How to Prepare
- Fasting is not required
- Inform the laboratory about any current vitamin D or biotin supplements
- For same-day reports, give the sample before 11 am from Monday to Saturday
- If home collection is booked, ensure the sampler visits at the scheduled time
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"In suspected vitamin D deficiency, the 25-hydroxy vitamin D level is the best overall indicator of body stores. LC-MS/MS provides high sensitivity and specificity for differentiating D2 and D3, enabling precise replacement therapy."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Improperly labelled specimen
- Hemolyzed sample
- Lipemic sample that interferes with analysis
- Inadequate sample volume below 1.5 mL
Understanding Your Results
High risk for bone disease; supplementation and clinical follow-up recommended
Status: Deficient
Level: < 20 ng/mL
Suboptimal vitamin D levels; corrective measures may be advised
Status: Insufficient
Level: 20–29 ng/mL
Adequate vitamin D status for most individuals
Status: Sufficient / Optimal
Level: 30–100 ng/mL
Potential vitamin D excess; reduce supplementation and re-evaluate
Status: Elevated
Level: > 100 ng/mL
Consult the referring physician or an endocrinologist to review your results, determine the need for vitamin D supplements or dietary changes, and schedule follow-up testing.
Limitations
- ⚠This test measures 25-hydroxyvitamin D, not the active 1,25-dihydroxyvitamin D form
- ⚠Results should be interpreted alongside serum calcium, phosphorus, PTH and clinical findings
- ⚠Very low or very high results may require confirmatory testing
- ⚠The test does not directly measure vitamin D from tissue stores
Risks & Considerations
- ●Minimal risk compared to daily blood sampling
- ●Possible mild bruising at the puncture site
- ●Dizziness or fainting during blood collection
- ●Rare infection or excessive bleeding at the site
Interfering Factors
- ●Delayed centrifugation or prolonged storage of raw blood
- ●Hemolysis or lipemia may interfere with assay
- ●Recent intake of high-dose vitamin D analogs may affect measured levels
- ●Biotin may interfere with some immunoassays; LC-MS/MS method minimizes this risk
Compare With Similar Tests
| Test | Vitamin D Ultrasensitive Test | Standard Vitamin D Test | Vitamin D Total (25-OH) Test | 1,25-Dihydroxy Vitamin D Test |
|---|---|---|---|---|
| Comparison | Vitamin D Ultrasensitive Test |
Frequently Asked Questions
What is the Vitamin D Ultrasensitive Test?
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Do I need to fast for the Vitamin D Ultrasensitive Test?
What sample is needed for the test?
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What are the normal Vitamin D levels?
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