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ZFYVE26 Gene SPG15 NGS Genetic Test

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ZFYVE26 Gene SPG15 NGS Genetic Test

Short Name: ZFYVE26 SPG15 NGS

Also known as: SPG15 Genetic Test, ZFYVE26 Gene Sequencing, Hereditary Spastic Paraplegia Type 15 NGS Test

ZFYVE26 Gene SPG15 NGS Genetic Test test available at DNA Labs India for ₹20,000. Uses Next-Generation Sequencing (NGS) on Blood or Extracted DNA or One drop Blood on FTA Card samples. Results in Results are typically available within 3 to 4 weeks after the sample is received at the laboratory.. Free home collection in 300+ cities across India.

NGS Genetic TestAll Ages🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SULOCHANA HEMCHANDRA HOLLA

Dr SULOCHANA HEMCHANDRA HOLLA

Consultant Medical Geneticist · Reg: 8532

Last reviewed: September 7, 2026

Overview

The purpose of the ZFYVE26 Gene SPG15 NGS Genetic Test is to confirm or rule out a clinical diagnosis of SPG15 by identifying disease-causing mutations in the ZFYVE26 gene. It helps in differentiating SPG15 from other forms of hereditary spastic paraplegia and supports informed treatment and reproductive decisions.

Test Code
4521
Price
₹20,000
Sample Type
Blood or Extracted DNA or One drop Blood on FTA Card
Result Time
Results are typically available within 3 to 4 weeks after the sample is received at the laboratory.
Fasting Required
No
Method
Next-Generation Sequencing (NGS)
Step 1

Sample Collection

A genetic counseling session is required prior to sample collection to document the clinical history and draw a pedigree chart of affected family members. No fasting is required for this test.

Method: Venipuncture or FTA card blood spot

Step 2

Laboratory Analysis

During sample collection, a healthcare professional will draw a peripheral blood sample from the arm or obtain a few drops of blood on an FTA card for the test.

Step 3

Report Delivery

After sample collection, the patient can resume normal activities without any restrictions. The sample is securely transported to the laboratory for analysis.

Timeline: Results are typically available within 3 to 4 weeks after the sample is received at the laboratory.

Patient Instructions

1
Before the Test:Prior to the test, patients undergo a genetic counseling session to review family history and understand the implications of the test results. No specific preparation such as fasting is required.
2
During the Test:A sample of blood (or extracted DNA) is collected; the process is quick and minimally invasive.
3
After the Test:Once the sample is taken, there are no restrictions. The patient will receive the report within 3 to 4 weeks over email/portal and can consult with a genetic counselor for interpretation.

About This Test

Who Should Get This Test

The purpose of the ZFYVE26 Gene SPG15 NGS Genetic Test is to confirm or rule out a clinical diagnosis of SPG15 by identifying disease-causing mutations in the ZFYVE26 gene. It helps in differentiating SPG15 from other forms of hereditary spastic paraplegia and supports informed treatment and reproductive decisions.

How to Prepare

  • Please bring all relevant medical records, neuroimaging reports, and family history information for the genetic counseling session
  • Ensure the FTA card is properly labeled with the patient's name, date, and time of collection
  • For blood samples, use an EDTA vacutainer to avoid clotting
  • Avoid sending samples that are hemolyzed or over-filled

Doctor's Notes

Reviewed by — MBBS, MD (Medical Genetics) · Reg. No. 8532

"Genetic testing for SPG15 not only confirms the diagnosis but also enables carrier testing and reproductive planning for affected families. I recommend NGS-based analysis of ZFYVE26 for patients presenting with early-onset progressive spastic paraplegia."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeBlood or Extracted DNA or One drop Blood on FTA Card
Sample VolumeAs required for extracted DNA (typically 2-5 mL blood or 1-2 FTA blood spots)
ContainerEDTA vacutainer (blood) / DNA vial (extracted DNA) / FTA card
Collection MethodVenipuncture or FTA card blood spot

Sample Stability

Whole blood (EDTA): 72 hours at room temperature (15-25°C) or 7 days at 2-8°C as per laboratory protocol
Extracted DNA: Stable for up to 1 year when stored at -20°C or below
FTA card blood spots: Stable for several months at room temperature
Sample Rejection Criteria:
  • Hemolyzed or clotted blood sample
  • Insufficient sample volume for DNA extraction
  • Improperly labeled sample (no identifier)
  • Incorrect sample container (e.g., serum tube instead of EDTA)

Understanding Your Results

The test report will be generated by a clinical geneticist and will include a clear interpretation of the NGS findings. The result may be classified as positive, negative, or as a variant of uncertain significance (VUS). A positive result confirms the presence of a pathogenic or likely pathogenic mutation in the ZFYVE26 gene, establishing the diagnosis of SPG15. A negative result reduces the likelihood of ZFYVE26-related SPG15 but does not entirely exclude it. VUS results require additional family studies to determine the clinical significance.
📊

Pathogenic or Likely Pathogenic Variant Detected

Confirms the diagnosis of ZFYVE26-associated SPG15. Genetic counseling is strongly recommended to discuss management and inheritance risks.

📊

Negative (No Pathogenic Variant Detected)

No disease-causing mutation was identified in the ZFYVE26 gene. Other genetic and non-genetic causes should be considered.

📊

Variant of Uncertain Significance (VUS)

A genetic change was found, but its clinical significance is currently unknown. Additional testing of family members may help clarify its role.

⚠️ When to Consult a Doctor:

If the test result is positive, please consult a neurologist and a clinical geneticist to discuss personalized management, surveillance, and family planning options. For a VUS result, let your doctor know to plan follow-up family testing and potential functional studies.

Limitations

  • ⚠This test analyses the ZFYVE26 gene only and will not detect mutations in other genes associated with hereditary spastic paraplegia
  • ⚠Large genomic rearrangements, deep intronic mutations, or regulatory region variants may not be identified by standard NGS
  • ⚠A negative result does not entirely exclude the possibility of SPG15, especially if sequencing coverage is incomplete or variant classification is uncertain
  • ⚠The clinical significance of variants of unknown significance may require further family studies and functional analysis

Risks & Considerations

  • ●No significant risks are associated with routine blood sampling, but mild bruising or bleeding may occur at the puncture site.
  • ●Genetic testing may reveal unexpected risk information for family members; this may pose psychological or social implications.
  • ●There is a possibility of discovering variants of uncertain significance which require further evaluation.

Interfering Factors

  • ●Sample degradation due to prolonged storage or high temperature
  • ●Presence of maternal cell contamination in fetal samples
  • ●Conflicting variants in pseudogenes or homologous regions
  • ●Incomplete clinical history leading to misinterpretation of variant significance

Frequently Asked Questions

What is SPG15?
SPG15 (Spastic Paraplegia Type 15) is a rare hereditary neurological disorder caused by mutations in the ZFYVE26 gene. It is characterized by progressive muscle stiffness and weakness in the legs, bladder dysfunction, and often cognitive impairment or peripheral neuropathy.
What is the cost of the ZFYVE26 Gene SPG15 NGS Genetic Test?
The total cost of the test is INR 20,000, which includes the genetic counseling session, NGS sequencing, clinical report, and raw data files. Free home sample collection is also included.
What sample is required for this test?
The test can be done on blood (EDTA tube), extracted DNA, or a few drops of blood on FTA card. All three sample types are acceptable for NGS analysis.
Do I need to fast before the test?
No, fasting is not required for this genetic test. You can eat and drink normally before sample collection.
How long does it take to get the test results?
The turnaround time is 3 to 4 weeks from the day the sample reaches the laboratory. You will receive an alert when the report is ready on the online portal.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection across many cities in India for this test when you book online.
Which cities are covered for home sample collection?
Home collection is available in Mumbai, Delhi, Bangalore, Hyderabad, Chennai, Kolkata, and many other Indian cities. A full list is shown on the booking page.
What technology is used for testing?
Next-Generation Sequencing (NGS) is used to analyze the coding regions and splice sites of the ZFYVE26 gene, ensuring high accuracy and comprehensive mutation detection.
Does this test detect all types of hereditary spastic paraplegia?
No, this test specifically analyzes the ZFYVE26 gene, which is associated with SPG15. For broader evaluation, an HSP gene panel or whole exome sequencing is recommended.
Why is genetic counseling required before the test?
Genetic counseling helps to document family history, draw a pedigree chart, explain the inheritance pattern, and discuss the medical and psychological impact of test results. It is essential for accurate interpretation and informed decision-making.
Will I receive raw sequencing data along with the report?
Yes, DNA Labs India transparently provides the Raw Data, FASTQ, and VCF files along with the clinical test report for this genetic test.
What does a negative result mean?
A negative result means no pathogenic mutation was identified in the ZFYVE26 gene. It significantly lowers but does not completely exclude SPG15. The doctor may recommend further genetic testing if symptoms persist.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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