Bacterial Genome De Novo Assembly and Annotation-Illumina Test
Short Name: Bacterial Genome Assembly
Also known as: Bacterial Whole Genome Sequencing, De Novo Bacterial Genome Assembly, Illumina Bacterial Genome Analysis
Bacterial Genome De Novo Assembly and Annotation-Illumina Test test available at DNA Labs India for ₹18,000. Uses Illumina Next-Generation Sequencing (NGS), De novo assembly, Genome annotation on Bacterial culture isolate (pure colony) or clinical specimen (e.g., blood, sputum, urine) as per physician's request samples. Results in Reports are typically available within 4 weeks from the date of sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
The primary purpose of bacterial genome de novo assembly and annotation is to obtain a complete and accurate genetic blueprint of a bacterial isolate. This is essential for: 1) Identifying the exact species and strain of bacteria, especially in cases where conventional methods are inconclusive. 2) Detecting antimicrobial resistance genes, guiding effective antibiotic therapy. 3) Understanding virulence factors and pathogenic mechanisms. 4) Conducting epidemiological surveillance and outbreak tracking. 5) Supporting research in bacterial evolution and comparative genomics. 6) Facilitating the development of novel diagnostics, vaccines, and therapeutics. By providing a comprehensive genomic map, this test empowers clinicians and researchers with actionable insights for improved patient outcomes and public health interventions.
- Test Code
- 6382
- CPT Code
- N/A
- ICD Code
- N/A
- Price
- ₹18,000
- Sample Type
- Bacterial culture isolate (pure colony) or clinical specimen (e.g., blood, sputum, urine) as per physician's request
- Result Time
- Reports are typically available within 4 weeks from the date of sample receipt.
- Fasting Required
- No
- Method
- Illumina Next-Generation Sequencing (NGS), De novo assembly, Genome annotation
Sample Collection
No special preparation is required. However, if a bacterial culture is being submitted, ensure it is a pure, fresh isolate. For clinical specimens, follow your physician's instructions.
Method: Specimen collection by healthcare professional
Laboratory Analysis
Specimen collection should be performed by a trained healthcare professional using sterile techniques to avoid contamination.
Report Delivery
The sample should be transported to the laboratory as soon as possible. If there is a delay, store the sample at 2-8°C for up to 24 hours. For culture isolates, maintain appropriate transport conditions.
Timeline: Reports are typically available within 4 weeks from the date of sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of bacterial genome de novo assembly and annotation is to obtain a complete and accurate genetic blueprint of a bacterial isolate. This is essential for: 1) Identifying the exact species and strain of bacteria, especially in cases where conventional methods are inconclusive. 2) Detecting antimicrobial resistance genes, guiding effective antibiotic therapy. 3) Understanding virulence factors and pathogenic mechanisms. 4) Conducting epidemiological surveillance and outbreak tracking. 5) Supporting research in bacterial evolution and comparative genomics. 6) Facilitating the development of novel diagnostics, vaccines, and therapeutics. By providing a comprehensive genomic map, this test empowers clinicians and researchers with actionable insights for improved patient outcomes and public health interventions.
How to Prepare
- For culture isolates: Use a sterile loop to transfer 1-2 colonies into a sterile tube containing transport medium.
- For clinical specimens: Collect in a sterile container as per standard protocols (e.g., blood in EDTA tube, sputum in sterile cup).
- Label the sample with patient name, ID, and date of collection.
- Maintain cold chain if transport time exceeds 2 hours.
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"Bacterial genome sequencing is a powerful tool for understanding antimicrobial resistance and virulence factors. This test provides critical data for clinical management and epidemiological studies."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample received in inappropriate container
- Sample leaked or contaminated
- Insufficient quantity of bacterial culture or specimen
- Sample hemolyzed (for blood)
- Sample not labeled correctly
- Sample stored improperly (e.g., at room temperature for >24 hours)
Understanding Your Results
High N50 and low contig count indicate a high-quality assembly, suitable for downstream analysis. Poor assembly may require resequencing.
Presence of resistance genes suggests potential resistance to corresponding antibiotics. This should be correlated with phenotypic susceptibility testing.
Detection of virulence genes indicates pathogenic potential. The specific genes present can inform on the severity of infection and clinical management.
The assigned sequence type helps in epidemiological tracking and outbreak investigations. Certain STs may be associated with high-risk clones.
If you have symptoms of a bacterial infection (fever, pain, swelling, etc.) or if you have been advised this test by a healthcare provider, consult your physician for proper clinical correlation and management. Do not self-interpret results.
Limitations
- ⚠De novo assembly may not resolve repetitive regions or plasmids completely
- ⚠Annotation accuracy depends on existing databases and may miss novel genes
- ⚠Results are for research and clinical guidance; not a standalone diagnostic test
- ⚠Requires a pure bacterial culture for optimal results; direct clinical specimens may have lower sensitivity
Risks & Considerations
- ●No significant risks are associated with this test as it is performed on a sample collected by standard procedures.
- ●If a blood sample is taken, there is a minimal risk of bruising or infection at the puncture site.
Interfering Factors
- ●Contamination of the bacterial culture with other organisms
- ●Insufficient quantity or poor quality of DNA
- ●Presence of PCR inhibitors in the sample
- ●Mixed bacterial populations
- ●Incomplete sequencing coverage due to technical issues
Compare With Similar Tests
| Test | Bacterial Genome De Novo Assembly and Annotation-Illumina | ||
|---|---|---|---|
| Comparison | Bacterial Genome De Novo Assembly and Annotation-Illumina |
Frequently Asked Questions
What is bacterial genome de novo assembly and annotation?
What is the cost of this test at DNA Labs India?
What is the turnaround time for results?
What sample is required for this test?
Is fasting required before the test?
Can this test detect antimicrobial resistance?
Is home sample collection available?
How should the sample be transported?
What is the difference between de novo assembly and reference-based assembly?
Can this test be used for outbreak investigations?
Are there any risks associated with this test?
How should I interpret the results?
Related Tests
FCGR2B Gene Malaria, resistance to NGS Genetic Test
₹20,000ICAM1 Gene Malaria, cerebral, susceptibility to NGS Genetic Test
₹20,000ITS Sequencing
₹5,600IL12RB2 Gene Atypical Mycobacterial infection, IL12RB2 related NGS Genetic Test
₹20,000Parvovirus B19 Qualitative PCR Test
₹5,000Respiratory Panel 5 Test
₹4,500Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
Book Your Test
Enter your details and we'll connect you within 15 minutes.