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DNA Labs India

Bacterial Genome De Novo Assembly and Annotation-Illumina and ONT Hybrid Test

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Bacterial Genome De Novo Assembly and Annotation-Illumina and ONT Hybrid Test

Short Name: Bacterial Genome Assembly

Also known as: Bacterial Whole Genome Sequencing, De Novo Bacterial Genome Assembly, Hybrid Genome Assembly

Bacterial Genome De Novo Assembly and Annotation-Illumina and ONT Hybrid Test test available at DNA Labs India for ₹45,000. Uses Illumina short-read sequencing, Oxford Nanopore long-read sequencing, Hybrid de novo assembly, Genome annotation on Bacterial culture isolate (pure colony) samples. Results in Reports are typically available within 4 weeks after the sample is received.. Free home collection in 300+ cities across India.

Genomic Sequencing🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SHAILAJA RAGHUNATH MURDESHWAR

Dr SHAILAJA RAGHUNATH MURDESHWAR

Consultant Physician · Reg: 8052

Last reviewed: September 7, 2026

Overview

The purpose of this test is to provide a complete and accurate genomic characterization of a bacterial isolate. It is used to identify bacterial strains that are difficult to culture or identify by conventional methods, to determine antimicrobial resistance profiles, to investigate outbreaks, and to support epidemiological studies. The hybrid assembly ensures high-quality genome sequences, enabling reliable annotation of genes and functional elements.

Test Code
6384
CPT Code
N/A
ICD Code
N/A
Price
₹45,000
Sample Type
Bacterial culture isolate (pure colony)
Result Time
Reports are typically available within 4 weeks after the sample is received.
Fasting Required
No
Method
Illumina short-read sequencing, Oxford Nanopore long-read sequencing, Hybrid de novo assembly, Genome annotation
Step 1

Sample Collection

Ensure the bacterial isolate is pure and viable. Subculture if necessary. Provide clinical history and suspected diagnosis.

Method: Clinical specimen collection by trained phlebotomist

Step 2

Laboratory Analysis

Collect the bacterial isolate using sterile technique. Place in appropriate transport medium and label correctly.

Step 3

Report Delivery

Transport the sample to the laboratory at room temperature or refrigerated (2-8°C) if delay is expected. Avoid freezing.

Timeline: Reports are typically available within 4 weeks after the sample is received.

Patient Instructions

1
Before the Test:No special preparation is required. The test is performed on a bacterial culture, so the patient does not need to fast or alter medications.
2
During the Test:The patient is not directly involved in the test. The laboratory processes the bacterial isolate.
3
After the Test:No specific post-test care is required. The patient should discuss results with their healthcare provider.

About This Test

Who Should Get This Test

The purpose of this test is to provide a complete and accurate genomic characterization of a bacterial isolate. It is used to identify bacterial strains that are difficult to culture or identify by conventional methods, to determine antimicrobial resistance profiles, to investigate outbreaks, and to support epidemiological studies. The hybrid assembly ensures high-quality genome sequences, enabling reliable annotation of genes and functional elements.

How to Prepare

  • Use sterile swabs or loops to collect isolated colonies
  • Place in sterile tube with transport medium (e.g., Amies)
  • Label with patient ID, date, and source
  • Transport to lab within 24 hours

Doctor's Notes

Reviewed by — MBBS, MD (General Medicine) · Reg. No. 8052

"Hybrid genome assembly provides comprehensive bacterial characterization, crucial for outbreak investigations and antimicrobial resistance surveillance."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeBacterial culture isolate (pure colony)
Sample Volume1-2 isolated colonies or 1 mL liquid culture
ContainerSterile tube with transport medium
Collection MethodClinical specimen collection by trained phlebotomist

Sample Stability

Room temperature
Refrigerated (2-8°C)
Frozen (-20°C)
Sample Rejection Criteria:
  • Mixed culture or contaminated sample
  • Insufficient quantity of bacterial growth
  • Sample received after prolonged delay without proper storage
  • Improper labeling

Understanding Your Results

The report provides a comprehensive genomic analysis of the bacterial isolate. Results are interpreted by clinical geneticists and infectious disease specialists to guide clinical decisions.
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⚠️ When to Consult a Doctor:

Consult your healthcare provider if you have symptoms of a bacterial infection, especially if they are recurrent, severe, or unresponsive to standard treatment. This test is typically ordered by specialists in infectious disease or clinical microbiology.

Limitations

  • ⚠Requires a pure bacterial culture; cannot be performed directly on clinical specimens
  • ⚠May not detect all resistance mechanisms if novel genes are present
  • ⚠Bioinformatics analysis depends on reference databases; novel genes may be missed
  • ⚠Cost may be prohibitive for routine use

Risks & Considerations

  • ●No direct risks to the patient as the test is performed on a bacterial culture
  • ●Potential for inconclusive results if the culture is contaminated

Interfering Factors

  • ●Contamination of the bacterial culture with other organisms
  • ●Poor DNA quality or quantity
  • ●Incomplete lysis of certain bacterial cell walls
  • ●Presence of inhibitors in the sample

Compare With Similar Tests

TestBacterial Genome De Novo Assembly and Annotation-Illumina and ONT Hybrid
ComparisonBacterial Genome De Novo Assembly and Annotation-Illumina and ONT Hybrid

Frequently Asked Questions

What is bacterial genome de novo assembly?
It is the process of reconstructing the complete genome sequence of a bacterium from sequencing reads without a reference genome. This provides a comprehensive genetic blueprint of the organism.
Why use hybrid sequencing (Illumina + ONT)?
Hybrid sequencing combines the high accuracy of Illumina short reads with the long-read capability of ONT, resulting in a complete, accurate genome assembly that is ideal for identifying resistance genes, virulence factors, and strain types.
What sample is required for this test?
A pure bacterial culture isolate (1-2 isolated colonies or 1 mL of liquid culture) is required. The sample must be collected and transported in a sterile container with appropriate transport medium.
How long does it take to get results?
The turnaround time is approximately 4 weeks from the time the sample is received at the laboratory.
What is the cost of the test?
The test costs INR 45000, which includes sequencing, bioinformatics analysis, and a detailed report.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
Can this test detect antimicrobial resistance?
Yes, the test identifies known antimicrobial resistance genes in the bacterial genome, which helps predict resistance to specific antibiotics.
Is this test useful for outbreak investigations?
Absolutely. The high-resolution genomic data allows for precise strain typing and phylogenetic analysis, which is essential for tracking outbreaks and understanding transmission.
What is the difference between de novo assembly and re-sequencing?
De novo assembly builds a genome from scratch without a reference, while re-sequencing maps reads to a known reference. De novo is essential for novel or divergent organisms.
Are there any risks to the patient?
No, the test is performed on a bacterial culture, so there are no direct risks to the patient.
How should the sample be transported?
The sample should be transported at room temperature or refrigerated (2-8°C) and must reach the laboratory within 24-72 hours. Do not freeze.
Will the report include interpretation?
Yes, the report includes a detailed interpretation by clinical geneticists and infectious disease specialists, including clinical implications.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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