Bacterial Genome Sequencing, De Novo Assembly and Annotation - Illumina and ONT Hybrid Test
Short Name: Bacterial Genome Sequencing (Hybrid)
Also known as: Bacterial Whole Genome Sequencing, WGS for Bacteria, Hybrid Bacterial Genome Sequencing
Bacterial Genome Sequencing, De Novo Assembly and Annotation - Illumina and ONT Hybrid Test test available at DNA Labs India for ₹125,000. Uses Illumina short-read sequencing, Oxford Nanopore Technologies (ONT) long-read sequencing, Hybrid de novo assembly, Genome annotation on Extracted DNA samples. Results in Reports are typically available within 8 weeks from the date of sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
The purpose of this test is to obtain a complete and accurate bacterial genome sequence for detailed genetic analysis. It aids in understanding bacterial biology, identifying antimicrobial resistance genes, tracking outbreaks, and characterizing virulence factors. In clinical settings, it can be used for definitive diagnosis of infections when conventional tests fail, and for guiding targeted therapy in complex cases.
- Test Code
- 6340
- CPT Code
- N/A
- ICD Code
- N/A
- Price
- ₹125,000
- Sample Type
- Extracted DNA
- Result Time
- Reports are typically available within 8 weeks from the date of sample receipt.
- Fasting Required
- No
- Method
- Illumina short-read sequencing, Oxford Nanopore Technologies (ONT) long-read sequencing, Hybrid de novo assembly, Genome annotation
Sample Collection
Ensure that the DNA sample is extracted from a pure bacterial culture or a clinical specimen as per laboratory instructions. Avoid repeated freeze-thaw cycles. Provide relevant clinical history and suspected organism information.
Method: DNA extraction from culture or clinical specimen
Laboratory Analysis
The sample (extracted DNA) will be collected by a trained phlebotomist or laboratory personnel. For home collection, the sample will be transported in appropriate cold conditions.
Report Delivery
No specific precautions are required after sample collection. The sample will be processed in the laboratory.
Timeline: Reports are typically available within 8 weeks from the date of sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this test is to obtain a complete and accurate bacterial genome sequence for detailed genetic analysis. It aids in understanding bacterial biology, identifying antimicrobial resistance genes, tracking outbreaks, and characterizing virulence factors. In clinical settings, it can be used for definitive diagnosis of infections when conventional tests fail, and for guiding targeted therapy in complex cases.
How to Prepare
- Sample must be labeled with patient ID and date
- Maintain cold chain during transport
- Avoid contamination by using sterile containers
- Provide clinical details and suspected organism
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"Hybrid sequencing provides comprehensive genomic data essential for outbreak investigations, antimicrobial resistance profiling, and virulence characterization."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient DNA quantity (<1 µg)
- Degraded DNA (fragmented)
- Contaminated sample
- Improper labeling or documentation
Understanding Your Results
Indicates high-quality assembly suitable for detailed analysis.
Suggests potential resistance to specific antibiotics; guide therapy accordingly.
Indicates pathogenic potential; may correlate with disease severity.
Helps in epidemiological tracking and outbreak investigation.
Consult your physician if you have persistent or severe symptoms of bacterial infection, or if you have been advised this test for diagnostic or research purposes. Discuss the results with your doctor to understand their implications for your health.
Limitations
- ⚠Requires high-quality extracted DNA; poor quality may lead to incomplete assembly
- ⚠Hybrid approach is more expensive than single-platform methods
- ⚠Annotation accuracy depends on existing databases
- ⚠Not a routine diagnostic test; reserved for complex cases
- ⚠Turnaround time is longer (8 weeks) compared to standard tests
Risks & Considerations
- ●No physical risks are associated with this test as it is performed on extracted DNA.
Interfering Factors
- ●DNA degradation or low concentration
- ●Contamination with host or other bacterial DNA
- ●Incomplete sequencing coverage due to technical issues
- ●Bioinformatics pipeline variations
Compare With Similar Tests
| Test | Bacterial Genome Sequencing, De Novo Assembly and Annotation - Illumina and ONT Hybrid | Illumina-only Sequencing | ONT-only Sequencing | 16S rRNA Gene Sequencing | Whole Genome Sequencing (Reference-based) |
|---|---|---|---|---|---|
| Comparison | Bacterial Genome Sequencing, De Novo Assembly and Annotation - Illumina and ONT Hybrid | Lower cost but shorter reads, making de novo assembly more challenging in repetitive regions. | Long reads but higher error rate, may require additional polishing. | Targets a single gene for species identification, not whole genome information. | Uses a reference genome for assembly, may miss novel sequences. |
Frequently Asked Questions
What is the difference between Illumina and ONT sequencing?
Why is de novo assembly important?
How long does it take to get results?
What type of sample is required?
Can this test detect antimicrobial resistance?
Is this test covered by insurance?
What is the cost of the test?
Do I need to fast before the test?
Can home sample collection be arranged?
What is the quality of the assembly?
Who will interpret the results?
Are there any risks associated with this test?
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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
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