Bacterial V3-V4 16S rRNA Amplicon Sequencing Test
Short Name: 16S rRNA Sequencing
Also known as: 16S rRNA Gene Sequencing, Bacterial Amplicon Sequencing, V3-V4 16S Sequencing
Bacterial V3-V4 16S rRNA Amplicon Sequencing Test test available at DNA Labs India for ₹7,000. Uses Next-Generation Sequencing (NGS), PCR Amplification on Extracted DNA samples. Results in Reports are typically delivered within 4 weeks after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of Bacterial V3-V4 16S rRNA amplicon sequencing is to identify the causative bacterial agent in patients with suspected bacterial infections, especially when conventional cultures fail. It helps in detecting uncommon, fastidious, or slow-growing bacteria, and provides a definitive diagnosis for targeted antimicrobial therapy. The test is also useful in epidemiological studies and outbreak investigations.
- Test Code
- 6361
- CPT Code
- N/A
- ICD Code
- N/A
- Price
- ₹7,000
- Sample Type
- Extracted DNA
- Result Time
- Reports are typically delivered within 4 weeks after sample collection.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS), PCR Amplification
Sample Collection
No special preparation is required. Inform your doctor about any antibiotics you are taking.
Method: Blood/Urine/Stool/Body fluid/Tissue
Laboratory Analysis
Sample collection is performed by a trained phlebotomist or healthcare professional.
Report Delivery
No specific aftercare required. You can resume normal activities immediately.
Timeline: Reports are typically delivered within 4 weeks after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of Bacterial V3-V4 16S rRNA amplicon sequencing is to identify the causative bacterial agent in patients with suspected bacterial infections, especially when conventional cultures fail. It helps in detecting uncommon, fastidious, or slow-growing bacteria, and provides a definitive diagnosis for targeted antimicrobial therapy. The test is also useful in epidemiological studies and outbreak investigations.
How to Prepare
- Sample type: Extracted DNA (provided by referring lab or collected at home)
- For home collection, a technician will visit your location
- Ensure the sample is transported in a sterile container
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"16S rRNA sequencing is invaluable for identifying fastidious or unculturable bacteria, guiding targeted antimicrobial therapy."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient quantity of DNA
- Degraded DNA
- Improper labeling
- Sample not stored at appropriate temperature
Understanding Your Results
No bacterial DNA detected
No bacterial infection identified; consider other etiologies.
Single bacterial species identified
Likely causative agent; guide targeted antibiotic therapy.
Multiple bacterial species identified
Polymicrobial infection; consider mixed infection management.
Consult your doctor if you have persistent symptoms of infection, such as fever, chills, or localized pain, especially if conventional tests are negative. Early diagnosis can lead to better treatment outcomes.
Limitations
- ⚠Cannot differentiate between live and dead bacteria
- ⚠May not detect all bacterial species due to database limitations
- ⚠Requires high-quality extracted DNA
- ⚠Not quantitative; does not provide bacterial load
- ⚠May not detect viruses or fungi
Risks & Considerations
- ●Minimal risk of bruising or bleeding at the collection site
- ●No significant risks associated with this test
Interfering Factors
- ●Contamination of sample with environmental bacteria
- ●Insufficient bacterial DNA in the sample
- ●Prior antibiotic therapy may reduce bacterial load
- ●Improper sample collection or storage
Compare With Similar Tests
| Test | Bacterial V3-V4 16S rRNA Amplicon Sequencing | Conventional Bacterial Culture | 16S rRNA Sequencing |
|---|---|---|---|
| Comparison | Bacterial V3-V4 16S rRNA Amplicon Sequencing |
Frequently Asked Questions
What is Bacterial V3-V4 16S rRNA Amplicon Sequencing?
Why is this test done?
What sample is required?
Do I need to fast before the test?
How long does it take to get results?
What is the cost of the test?
Is home sample collection available?
Can this test detect all bacteria?
Is the test covered by insurance?
What are the symptoms of a bacterial infection?
How is the test performed?
Are there any risks?
Related Tests
FCGR2B Gene Malaria, resistance to NGS Genetic Test
₹20,000ICAM1 Gene Malaria, cerebral, susceptibility to NGS Genetic Test
₹20,000ITS Sequencing
₹5,600IL12RB2 Gene Atypical Mycobacterial infection, IL12RB2 related NGS Genetic Test
₹20,000Parvovirus B19 Qualitative PCR Test
₹5,000Respiratory Panel 5 Test
₹4,500Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
Book Your Test
Enter your details and we'll connect you within 15 minutes.