Bacterial V3-V4 16S rRNA Amplicon Sequencing Data Analysis Test
Short Name: 16S rRNA Sequencing
Also known as: 16S rRNA Gene Sequencing, Bacterial Identification by NGS, Microbiome Analysis
Bacterial V3-V4 16S rRNA Amplicon Sequencing Data Analysis Test test available at DNA Labs India for ₹5,000. Uses Next-Generation Sequencing (NGS), 16S rRNA Amplicon Sequencing on Extracted DNA samples. Results in Reports are typically delivered within 4 weeks after the sample is received by the laboratory.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The primary purpose of this test is to identify and classify bacterial strains present in a clinical specimen by sequencing the V3-V4 region of the 16S rRNA gene. It is used to diagnose bacterial infections, especially those that are difficult to culture, identify mixed infections, and provide information for targeted antimicrobial therapy. The test also helps in epidemiological studies and microbiome analysis.
- Test Code
- 6379
- CPT Code
- N/A
- ICD Code
- N/A
- Price
- ₹5,000
- Sample Type
- Extracted DNA
- Result Time
- Reports are typically delivered within 4 weeks after the sample is received by the laboratory.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS), 16S rRNA Amplicon Sequencing
Sample Collection
No special preparation is required. However, inform your doctor about any antibiotics you are taking, as this may affect the test results.
Method: Blood, swab, or body fluid as per clinical need
Laboratory Analysis
Sample collection is performed by a trained phlebotomist or healthcare professional. The type of sample (blood, swab, fluid) depends on the clinical indication.
Report Delivery
No specific aftercare is needed. You can resume normal activities immediately.
Timeline: Reports are typically delivered within 4 weeks after the sample is received by the laboratory.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of this test is to identify and classify bacterial strains present in a clinical specimen by sequencing the V3-V4 region of the 16S rRNA gene. It is used to diagnose bacterial infections, especially those that are difficult to culture, identify mixed infections, and provide information for targeted antimicrobial therapy. The test also helps in epidemiological studies and microbiome analysis.
How to Prepare
- Ensure the sample is collected in a sterile container
- Label the container with patient details
- Transport the sample to the lab at the recommended temperature (2-8°C if not processed immediately)
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"This advanced molecular test is invaluable for identifying bacteria that are difficult to culture or when a precise species-level identification is needed for targeted therapy. It helps in managing infections with precision, reducing the risk of antibiotic resistance."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient DNA quantity (<10 ng/µL)
- Degraded DNA (e.g., repeated freeze-thaw)
- Improperly labeled or contaminated samples
- Samples received in inappropriate transport media
Understanding Your Results
Likely causative pathogen; targeted therapy can be initiated.
Polymicrobial infection; consider combination therapy or further testing.
May indicate non-bacterial etiology or low bacterial load; clinical correlation required.
May be normal flora or contamination; interpret in clinical context.
Consult your physician if you have persistent symptoms of infection, or if you have received this test and need help interpreting the results. Seek immediate medical attention if you experience severe symptoms such as high fever, difficulty breathing, or confusion.
Limitations
- ⚠Cannot differentiate between live and dead bacteria
- ⚠May not detect all bacterial species due to primer bias
- ⚠Requires sufficient bacterial DNA for reliable results
- ⚠Interpretation may be limited by reference databases
- ⚠Not a substitute for culture-based susceptibility testing
Risks & Considerations
- ●Minimal risk of bruising or bleeding at the collection site
- ●Infection at the collection site (rare)
- ●No significant risks associated with the test itself
Interfering Factors
- ●Contamination during sample collection or processing
- ●Low DNA quantity or quality
- ●Presence of host DNA (e.g., human DNA) reducing bacterial signal
- ●PCR inhibitors in the sample (e.g., heme, bile salts)
- ●Prior antibiotic therapy may reduce bacterial load
Compare With Similar Tests
| Test | Bacterial V3-V4 16S rRNA Amplicon Sequencing Data Analysis | Culture and Sensitivity | PCR (Targeted) | Sanger Sequencing |
|---|---|---|---|---|
| Comparison | Bacterial V3-V4 16S rRNA Amplicon Sequencing Data Analysis |
Frequently Asked Questions
What is the cost of the Bacterial V3-V4 16S rRNA Amplicon Sequencing Data Analysis?
What sample is required for this test?
How long does it take to get the results?
Is fasting required before the test?
Can this test identify all types of bacteria?
Is home sample collection available?
What is the difference between this test and a routine culture?
Can this test detect antibiotic resistance?
Who should consider this test?
Are there any risks associated with the test?
How should I prepare for the test?
Will my insurance cover this test?
Related Tests
FCGR2B Gene Malaria, resistance to NGS Genetic Test
₹20,000ICAM1 Gene Malaria, cerebral, susceptibility to NGS Genetic Test
₹20,000ITS Sequencing
₹5,600IL12RB2 Gene Atypical Mycobacterial infection, IL12RB2 related NGS Genetic Test
₹20,000Parvovirus B19 Qualitative PCR Test
₹5,000Respiratory Panel 5 Test
₹4,500Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
Book Your Test
Enter your details and we'll connect you within 15 minutes.