Bordetella Multiplex (Bordetella Pertussis, Legionella Pneumophilia, K. Pneumonia) Detection & Differentiation (RNA Detection) Qualitative Test
Short Name: Bordetella Multiplex RNA Test
Also known as: Whooping Cough RNA Test, Legionnaires' Disease PCR Test, Klebsiella pneumoniae Detection Test
Bordetella Multiplex (Bordetella Pertussis, Legionella Pneumophilia, K. Pneumonia) Detection & Differentiation (RNA Detection) Qualitative Test test available at DNA Labs India for ₹11,900. Uses Real Time PCR on Nasopharyngeal secretions, swabs, oral/rectal swabs, blood, urine, sputum, throat swab, bronchoalveolar lavage, lung tissue samples. Results in Results are available by the 4th working day. Email reports within 48 hours, and phone reports within 36 hours after completion.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of this test is to diagnose and differentiate infections caused by Bordetella pertussis, Legionella pneumophila, and Klebsiella pneumoniae through RNA detection, enabling targeted treatment and management of respiratory illnesses.
- Test Code
- 3268
- Price
- ₹11,900
- Sample Type
- Nasopharyngeal secretions, swabs, oral/rectal swabs, blood, urine, sputum, throat swab, bronchoalveolar lavage, lung tissue
- Result Time
- Results are available by the 4th working day. Email reports within 48 hours, and phone reports within 36 hours after completion.
- Fasting Required
- No
- Method
- Real Time PCR
Sample Collection
Ensure patient signs consent document and provides clinical history. Avoid eating or drinking for 30 minutes before throat swab collection if applicable.
Method: Swab collection or as per sample type
Laboratory Analysis
Collect samples using sterile techniques as per standard protocols. For swabs, rotate gently in the nasopharynx or throat. For other samples, follow aseptic methods.
Report Delivery
Label samples correctly and transport immediately to the lab. Maintain cold chain if required. Inform the lab of any special handling needs.
Timeline: Results are available by the 4th working day. Email reports within 48 hours, and phone reports within 36 hours after completion.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this test is to diagnose and differentiate infections caused by Bordetella pertussis, Legionella pneumophila, and Klebsiella pneumoniae through RNA detection, enabling targeted treatment and management of respiratory illnesses.
How to Prepare
- Use sterile swabs for nasopharyngeal or throat samples
- Collect blood in appropriate anticoagulant tubes
- Sputum should be expectorated into a sterile container
- Bronchoalveolar lavage should be collected by trained personnel
- Store samples at 2-8°C if not processed immediately
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"This multiplex RNA test is essential for rapid and precise diagnosis of respiratory infections caused by Bordetella pertussis, Legionella pneumophila, and Klebsiella pneumoniae, enabling targeted antibiotic therapy and improving patient outcomes."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Leaking or unlabeled samples
- Samples collected in non-sterile containers
- Samples with visible contamination
- Samples beyond stability period
Understanding Your Results
Bordetella pertussis Detected
Indicates active infection with Bordetella pertussis, commonly causing whooping cough. Clinical correlation and treatment are recommended.
Legionella pneumophila Detected
Suggests Legionnaires' disease. Immediate medical intervention is advised due to severity.
Klebsiella pneumoniae Detected
Points to Klebsiella pneumoniae infection, which may require specific antibiotics. Assess for complications.
All Not Detected
No RNA from these bacteria detected. Consider other pathogens or repeat testing if symptoms persist.
Consult a doctor if you experience persistent cough, high fever, difficulty breathing, chest pain, or if symptoms worsen despite treatment. Early consultation is crucial for immunocompromised individuals.
Limitations
- ⚠Qualitative results only; does not quantify bacterial load
- ⚠May not detect all strains or variants of the bacteria
- ⚠False negatives possible if sample quality is poor
- ⚠Not a substitute for clinical evaluation and imaging studies
Risks & Considerations
- ●Minimal risk from swab collection, such as slight discomfort
- ●Rare risk of infection from blood draw
- ●No significant risks associated with the test itself
Interfering Factors
- ●Sample contamination during collection
- ●Improper storage or transport of samples
- ●Presence of inhibitors in the sample
- ●Recent antibiotic use may affect RNA detection
Compare With Similar Tests
| Test | Bordetella Multiplex (Bordetella Pertussis, Legionella Pneumophilia, K. Pneumonia) Detection & Differentiation (RNA Detection) Qualitative Test | Culture Test | Serology Test | Single PCR Test | Rapid Antigen Test |
|---|---|---|---|---|---|
| Comparison | Bordetella Multiplex (Bordetella Pertussis, Legionella Pneumophilia, K. Pneumonia) Detection & Differentiation (RNA Detection) Qualitative Test |
Frequently Asked Questions
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