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Bordetella Multiplex (Bordetella toxin, B. Pertussis, B. Holmensii, B. Parapolis) Detection & Differentiation (RNA Detection) Qualitative Test

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Bordetella Multiplex (Bordetella toxin, B. Pertussis, B. Holmensii, B. Parapolis) Detection & Differentiation (RNA Detection) Qualitative Test

Short Name: Bordetella Multiplex RNA Test

Also known as: Bordetella Multiplex PCR Test, Whooping Cough Multiplex RNA Test, Pertussis RNA Detection and Differentiation Test, Bordetella Species Identification Test, Bordetella Toxin Gene Detection Test

Bordetella Multiplex (Bordetella toxin, B. Pertussis, B. Holmensii, B. Parapolis) Detection & Differentiation (RNA Detection) Qualitative Test test available at DNA Labs India for ₹11,560. Uses Real-Time PCR (Polymerase Chain Reaction), RNA Detection Technology, Multiplex Qualitative Assay on Nasopharyngeal secretions, Nasopharyngeal swabs, Oral/rectal swabs, Throat swab, Sputum, Bronchoalveolar lavage (BAL), Blood, Urine, Lung tissue, Necropsy samples samples. Results in Results are typically available on the 4th working day. Email reports are dispatched within 48 hours of report generation. Phone results can be obtained within 36 hours.. Free home collection in 300+ cities across India.

Physician ReferredAll Ages🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Kothari Ramesh Shantilal

Dr Kothari Ramesh Shantilal

Consultant Microbiologist · Reg: 27670

Last reviewed: September 7, 2026

Overview

The primary purpose of the Bordetella Multiplex RNA Detection & Differentiation Test is to accurately identify and differentiate between Bordetella pertussis, Bordetella holmesii, Bordetella parapertussis, and the Bordetella toxin gene using molecular RNA detection. This test aids clinicians in confirming the diagnosis of pertussis (whooping cough) and related Bordetella infections, guiding appropriate antibiotic therapy, facilitating infection control measures, and supporting public health surveillance and outbreak investigations.

Test Code
3284
CPT Code
87798
ICD Code
A37.90
Price
₹11,560
Sample Type
Nasopharyngeal secretions, Nasopharyngeal swabs, Oral/rectal swabs, Throat swab, Sputum, Bronchoalveolar lavage (BAL), Blood, Urine, Lung tissue, Necropsy samples
Result Time
Results are typically available on the 4th working day. Email reports are dispatched within 48 hours of report generation. Phone results can be obtained within 36 hours.
Fasting Required
No
Method
Real-Time PCR (Polymerase Chain Reaction), RNA Detection Technology, Multiplex Qualitative Assay
Step 1

Sample Collection

No fasting is required. A signed consent document is mandatory. Bring any relevant clinical history, vaccination records, and prior test results. Inform the healthcare provider about any recent antibiotic use.

Method: Nasopharyngeal swab collection by trained phlebotomist or physician

Step 2

Laboratory Analysis

A trained phlebotomist or healthcare professional will collect a nasopharyngeal swab by gently inserting a flexible swab into the nostril and rotating it against the nasopharyngeal wall. The procedure takes approximately 1–2 minutes and may cause mild discomfort.

Step 3

Report Delivery

The sample is placed in viral transport medium and shipped immediately to the laboratory. Mild nasal discomfort or a slight nosebleed may occur rarely. No special post-collection care is typically needed.

Timeline: Results are typically available on the 4th working day. Email reports are dispatched within 48 hours of report generation. Phone results can be obtained within 36 hours.

Patient Instructions

1
Before the Test:No fasting is required. A signed consent document must be provided. Bring your clinical history, vaccination records, and any prior test results. Inform the healthcare provider about recent antibiotic use, as this may affect test sensitivity.
2
During the Test:A nasopharyngeal swab will be collected by a trained healthcare professional. The swab is gently inserted through the nostril to the nasopharyngeal area, rotated briefly, and then placed in viral transport medium. The procedure takes 1–2 minutes and may cause mild discomfort or a gagging sensation.
3
After the Test:The sample is immediately transported to the molecular microbiology laboratory for RNA extraction and Real-Time PCR analysis. Mild nasal discomfort may occur temporarily. No special post-test care is required. Results are available within 4 working days.

About This Test

Who Should Get This Test

The primary purpose of the Bordetella Multiplex RNA Detection & Differentiation Test is to accurately identify and differentiate between Bordetella pertussis, Bordetella holmesii, Bordetella parapertussis, and the Bordetella toxin gene using molecular RNA detection. This test aids clinicians in confirming the diagnosis of pertussis (whooping cough) and related Bordetella infections, guiding appropriate antibiotic therapy, facilitating infection control measures, and supporting public health surveillance and outbreak investigations.

How to Prepare

  • Sign the consent document before sample collection
  • Provide complete clinical history including symptom onset, duration, and vaccination status
  • Inform the phlebotomist about any recent antibiotic therapy
  • Avoid eating, drinking, or nasal irrigation for at least 1 hour before nasopharyngeal swab collection
  • Sample should be collected preferably within the first 3 weeks of symptom onset for optimal sensitivity
  • Ensure sample is properly labeled with patient details and date/time of collection

Doctor's Notes

Reviewed by — MBBS, MD (Microbiology) · Reg. No. 27670

"Bordetella pertussis remains a significant cause of respiratory morbidity worldwide, particularly in unvaccinated or partially vaccinated infants. The Bordetella Multiplex RNA Detection Test is a valuable diagnostic tool because it can simultaneously detect and differentiate multiple Bordetella species and the pertussis toxin gene in a single assay. Unlike conventional culture, which can take up to 7–10 days and has limited sensitivity, this Real-Time PCR-based RNA detection method provides rapid and highly sensitive results. This is especially important for early initiation of appropriate antibiotic therapy and timely public health interventions such as contact tracing. I recommend this test for patients presenting with paroxysmal cough, post-tussive vomiting, or apneic episodes, as well as for outbreak investigations. Early diagnosis significantly reduces transmission and complications, particularly in vulnerable populations including neonates, pregnant women, and immunocompromised individuals."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeNasopharyngeal secretions, Nasopharyngeal swabs, Oral/rectal swabs, Throat swab, Sputum, Bronchoalveolar lavage (BAL), Blood, Urine, Lung tissue, Necropsy samples
Sample VolumeAs per sample type — typically 1–2 mL for fluid specimens or one swab in transport media
ContainerSterile viral transport medium (VTM) tube or sterile leak-proof container
Collection MethodNasopharyngeal swab collection by trained phlebotomist or physician

Sample Stability

Nasopharyngeal swab in VTM: Stable for 24–48 hours at 2–8°C
Sputum/BAL: Stable for 24 hours at 2–8°C
Blood/Urine: Process within 6 hours of collection at 2–8°C
All samples should be transported to the laboratory as soon as possible after collection
Sample Rejection Criteria:
  • Unlabeled or mislabeled samples
  • Samples collected in expired or inappropriate transport media
  • Visibly contaminated or leaked samples
  • Insufficient sample volume
  • Samples received beyond the acceptable stability window
  • Samples without a signed consent form

Understanding Your Results

The Bordetella Multiplex RNA Detection Test provides qualitative results (Detected / Not Detected) for each of the four targets. Results should be interpreted by a qualified physician in the context of the patient's clinical presentation, exposure history, vaccination status, and other laboratory findings.
📊

Bordetella pertussis RNA: Detected

Active B. pertussis infection is indicated. This is the primary cause of whooping cough (pertussis). Immediate clinical correlation and initiation of appropriate antibiotic therapy (e.g., macrolides) is recommended. Contact tracing and public health notification may be required.

📊

Bordetella parapertussis RNA: Detected

B. parapertussis infection is indicated. This organism causes a milder pertussis-like illness. Clinical management is similar but generally associated with less severe outcomes compared to B. pertussis.

📊

Bordetella holmesii RNA: Detected

B. holmesii infection is indicated. This species has been associated with pertussis-like respiratory illness and bacteremia, particularly in asplenic or immunocompromised patients. Clinical correlation is essential.

📊

Bordetella Toxin Gene: Detected

Detection of the pertussis toxin gene indicates the presence of a virulent Bordetella strain capable of producing pertussis toxin. This is most commonly associated with B. pertussis and supports the diagnosis of active pertussis.

📊

All Targets: Not Detected

No Bordetella RNA was detected in the sample. Bordetella infection is unlikely but cannot be entirely excluded, especially if the sample was collected late in the illness or after antibiotic treatment. Clinical correlation and additional testing may be warranted if symptoms persist.

⚠️ When to Consult a Doctor:

Consult an infectious disease specialist or your physician immediately if the test result is positive for any Bordetella target. Early antibiotic treatment is critical, especially for infants, pregnant women, elderly patients, and immunocompromised individuals. If the test is negative but symptoms persist or worsen, consult your doctor for further evaluation and alternative diagnostic testing.

Limitations

  • ⚠This is a qualitative test and does not quantify the bacterial load
  • ⚠Cannot distinguish between viable and non-viable organisms as it detects RNA
  • ⚠Results should always be interpreted in conjunction with clinical presentation and patient history
  • ⚠A negative result does not completely rule out Bordetella infection, especially if the sample was collected late in the disease course
  • ⚠Cross-reactivity with closely related Bordetella species has been minimized but cannot be entirely excluded

Risks & Considerations

  • ●Mild nasal discomfort or irritation during nasopharyngeal swab collection
  • ●Rare minor nosebleed (epistaxis) from swab insertion
  • ●Temporary gagging sensation during sample collection
  • ●Extremely rare risk of nasal mucosal injury with improper technique

Interfering Factors

  • ●Recent antibiotic use (especially macrolides) may reduce bacterial load and affect detection
  • ●Improper sample collection technique or inadequate sample quality
  • ●Sample collected too late in the illness course (beyond 3–4 weeks of symptom onset)
  • ●Contamination of sample during collection or transport
  • ●Use of inappropriate transport media or delayed sample processing

Compare With Similar Tests

TestBordetella Multiplex (Bordetella toxin, B. Pertussis, B. Holmensii, B. Parapolis) Detection & Differentiation (RNA Detection) Qualitative TestBordetella CultureSingle-Target Bordetella PCRBordetella Serology (IgM/IgG)Direct Fluorescent Antibody (DFA) Test
ComparisonBordetella Multiplex (Bordetella toxin, B. Pertussis, B. Holmensii, B. Parapolis) Detection & Differentiation (RNA Detection) Qualitative Test

Frequently Asked Questions

What is the Bordetella Multiplex RNA Detection & Differentiation Test?
The Bordetella Multiplex RNA Detection & Differentiation Test is a molecular diagnostic test that uses Real-Time PCR technology to detect and differentiate RNA from Bordetella pertussis, Bordetella holmesii, Bordetella parapertussis, and the Bordetella toxin gene. It helps diagnose pertussis (whooping cough) and related Bordetella infections accurately and rapidly.
Why is this test recommended by doctors?
This test is recommended when a patient presents with persistent or paroxysmal cough, suspected whooping cough, or respiratory infection of unknown cause. It is also used during pertussis outbreaks for contact tracing and in immunocompromised patients with chronic respiratory symptoms. It provides faster and more specific results than conventional culture methods.
What sample types are accepted for this test?
Accepted sample types include nasopharyngeal secretions, nasopharyngeal swabs, oral/rectal swabs, throat swabs, sputum, bronchoalveolar lavage (BAL), blood, urine, lung tissue, and necropsy samples. Nasopharyngeal swabs are the most commonly used and preferred specimen type.
How long does it take to get the results?
Results are typically available within 4 working days from the date of sample collection. Email reports are sent within 48 hours of report generation, and phone results can be obtained within 36 hours.
Is fasting required before this test?
No, fasting is not required for the Bordetella Multiplex RNA Detection Test. However, it is advisable to avoid eating, drinking, or nasal irrigation for at least 1 hour before nasopharyngeal swab collection to ensure optimal sample quality.
What is the cost of the Bordetella Multiplex RNA Detection Test at DNA Labs India?
The cost of the Bordetella Multiplex RNA Detection & Differentiation Test at DNA Labs India is INR 11,560. This price includes test processing, free home sample collection, and digital report delivery.
Is home sample collection available for this test?
Yes, DNA Labs India offers free home sample collection for this test across India. You can book online and a trained phlebotomist will visit your location to collect the sample. Home collection is available in all major cities and many smaller towns across India.
What are the common symptoms of Bordetella infection?
Common symptoms of Bordetella infection include persistent paroxysmal cough (often with a characteristic whoop), runny nose, low-grade fever, sneezing, sore throat, difficulty breathing, post-tussive vomiting, and fatigue. In infants, apneic episodes (pauses in breathing) may occur. Symptoms can vary depending on the Bordetella species involved.
How accurate is the Bordetella Multiplex RNA Detection Test?
This test uses Real-Time PCR technology, which is considered the gold standard for molecular detection of Bordetella species. It offers high sensitivity and specificity, significantly outperforming conventional culture and direct fluorescent antibody (DFA) methods. RNA detection ensures identification of the organism even at low concentrations in clinical specimens.
What is the difference between this test and a Bordetella culture?
A Bordetella culture involves growing the bacteria on specialized media and can take 7–10 days, with limited sensitivity (especially after antibiotic use or late in the illness). The Bordetella Multiplex RNA Detection Test uses Real-Time PCR to detect bacterial RNA directly, providing results within 4 working days with significantly higher sensitivity and the ability to differentiate between multiple Bordetella species simultaneously.
Can this test detect whooping cough (pertussis)?
Yes, this test can detect whooping cough (pertussis) by identifying Bordetella pertussis RNA and the Bordetella toxin gene. B. pertussis is the primary causative agent of pertussis. The test can also differentiate B. pertussis from other Bordetella species that may cause pertussis-like illness, such as B. parapertussis and B. holmesii.
Who should get the Bordetella Multiplex RNA Detection Test?
This test is recommended for individuals with persistent cough (especially lasting more than 2 weeks), suspected whooping cough, neonates and infants with respiratory symptoms or apneic episodes, immunocompromised patients with chronic respiratory illness, individuals identified during contact tracing in pertussis outbreaks, and anyone whose physician suspects a Bordetella infection based on clinical presentation.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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