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Bordetella Pertussis (RNA Detection) Qualitative Test

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Bordetella Pertussis (RNA Detection) Qualitative Test

Short Name: Bordetella Pertussis RNA Test

Also known as: Whooping Cough RNA Test, Pertussis PCR Test

Bordetella Pertussis (RNA Detection) Qualitative Test test available at DNA Labs India for ₹5,780. Uses Real Time PCR on Nasopharyngeal secretions, swabs, oral/rectal swabs, sputum, throat swab, bronchoalveolar lavage, lung tissue samples. Results in Results available in 4 working days via online portal, email, or WhatsApp.. Free home collection in 300+ cities across India.

Qualitative PCR🏠 Home Collection

🩺 Medically Reviewed By

Overview

The purpose of the Bordetella pertussis (RNA Detection) Qualitative Test is to provide a definitive diagnosis of whooping cough by detecting the genetic material (RNA) of Bordetella pertussis in respiratory samples. This aids in confirming active infections, guiding treatment decisions, and implementing infection control measures.

Test Code
3270
Price
₹5,780
Sample Type
Nasopharyngeal secretions, swabs, oral/rectal swabs, sputum, throat swab, bronchoalveolar lavage, lung tissue
Result Time
Results available in 4 working days via online portal, email, or WhatsApp.
Fasting Required
No
Method
Real Time PCR
Step 1

Sample Collection

Avoid eating, drinking, or smoking for at least 1 hour before sample collection. Inform the healthcare provider about any recent antibiotic use or medical history.

Method: Swab collection

Step 2

Laboratory Analysis

A healthcare professional will collect nasopharyngeal or throat swabs using sterile techniques. The procedure may cause mild discomfort but is generally quick.

Step 3

Report Delivery

Apply gentle pressure if any bleeding occurs. Resume normal activities immediately. Store the sample as instructed for transport.

Timeline: Results available in 4 working days via online portal, email, or WhatsApp.

Patient Instructions

1
Before the Test:No specific preparation required, but avoid eating or drinking for 1 hour before sample collection.
2
During the Test:Sample collection involves swabbing the nasopharynx or throat, which may cause mild discomfort.
3
After the Test:Resume normal activities. Results will be available in 4 working days.

About This Test

Who Should Get This Test

The purpose of the Bordetella pertussis (RNA Detection) Qualitative Test is to provide a definitive diagnosis of whooping cough by detecting the genetic material (RNA) of Bordetella pertussis in respiratory samples. This aids in confirming active infections, guiding treatment decisions, and implementing infection control measures.

How to Prepare

  • Use sterile swabs for nasopharyngeal or throat collection
  • Label the sample container with patient details
  • Transport the sample to the laboratory within the specified stability period
  • Follow aseptic techniques to prevent contamination

Doctor's Notes

Reviewed by — MBBS, MD (Microbiology) · Reg. No. 27670

"This test is crucial for early diagnosis of whooping cough, especially in infants, to prevent severe complications and guide appropriate treatment."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeNasopharyngeal secretions, swabs, oral/rectal swabs, sputum, throat swab, bronchoalveolar lavage, lung tissue
Sample VolumeAs required
ContainerSterile transport container
Collection MethodSwab collection

Sample Stability

Room temperature: 24 hours
Refrigerated (2-8°C): 48 hours
Frozen (-20°C): 7 days
Sample Rejection Criteria:
  • Improperly labeled or unlabeled samples
  • Leaking or damaged containers
  • Samples exceeding stability time
  • Contaminated or insufficient sample volume

Understanding Your Results

Results from the Bordetella pertussis RNA Detection Qualitative Test indicate the presence or absence of the bacterium's genetic material in the sample.
📊

Detected

Positive for Bordetella pertussis RNA, suggesting active infection. Clinical correlation is recommended.

Action: Consult a healthcare provider for diagnosis confirmation and treatment initiation.

📊

Not Detected

Negative for Bordetella pertussis RNA. Does not completely rule out infection if clinical suspicion remains high.

Action: Consider repeat testing or alternative diagnostic methods if symptoms persist.

⚠️ When to Consult a Doctor:

Consult a doctor immediately if you experience severe coughing fits, whooping sound, vomiting after coughing, or if an infant shows respiratory distress, regardless of test results.

Limitations

  • May not detect low levels of Bordetella pertussis RNA
  • False negatives possible in early or late stages of infection
  • Cannot distinguish between active infection and colonization
  • Limited to qualitative detection; does not quantify bacterial load
  • Requires specialized laboratory equipment and expertise

Risks & Considerations

  • Mild discomfort or gagging during swab collection
  • Rare risk of nosebleed from nasopharyngeal swab
  • Minimal risk of infection from improper collection

Interfering Factors

  • Improper sample collection technique
  • Contamination during sample handling
  • Recent antibiotic use may reduce bacterial load
  • Sample degradation due to delayed transport
  • Presence of inhibitors in respiratory specimens

Frequently Asked Questions

What is the Bordetella Pertussis RNA Detection Qualitative Test?
It is a laboratory test that uses Real Time PCR to detect the RNA of Bordetella pertussis in respiratory samples, helping diagnose whooping cough.
How is the test performed?
A healthcare professional collects a nasopharyngeal or throat swab, which is then analyzed in the lab using molecular techniques to identify bacterial RNA.
What samples are required for this test?
Nasopharyngeal secretions, swabs, oral/rectal swabs, sputum, throat swab, bronchoalveolar lavage, or lung tissue samples can be used.
Is fasting required before the test?
No, fasting is not required, but it is recommended to avoid eating or drinking for at least 1 hour before sample collection.
How long does it take to get results?
Results are typically available within 4 working days, with reports delivered via online portal, email, or WhatsApp.
What does a positive result mean?
A positive result indicates the presence of Bordetella pertussis RNA, suggesting an active infection. Consult a doctor for diagnosis and treatment.
What does a negative result mean?
A negative result means no Bordetella pertussis RNA was detected. However, if symptoms persist, repeat testing or other diagnostics may be needed.
Is the test accurate?
Yes, the test is highly specific and sensitive due to Real Time PCR technology, but no test is 100% accurate. Clinical correlation is important.
Can the test be done at home?
Yes, DNA Labs India offers free home sample collection for this test in many cities across India.
What is the cost of the test?
The cost is INR 5780, which includes sample collection, processing, and report delivery.
Is the test covered by insurance?
Coverage depends on your insurance plan. It is not typically covered under government schemes like PMJAY or CGHS, but check with your provider.
How should I prepare for the test?
No special preparation is needed, but inform your healthcare provider about any medications or medical history. Avoid eating or drinking for 1 hour before collection.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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Your Data Privacy

Your medical data is protected under Indian law.

Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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