Cell Line Authentication DNA Test
Short Name: Cell Line Authentication
Also known as: Cell Line Identity Testing, STR Profiling for Cell Lines
Cell Line Authentication DNA Test test available at DNA Labs India for ₹6,500. Uses STR DNA Technology on Extracted DNA, Cell Pellet, FTA Spot, or Tissue/Tumor samples. Results in Reports are typically available within 10-12 days after sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The purpose of the Cell Line Authentication DNA Test is to verify the identity and purity of cell lines used in research and clinical applications. It detects misidentification, cross-contamination, and genetic alterations that may occur during cell culture. The test uses STR profiling to generate a unique DNA fingerprint of the cell line, which is then compared to reference databases (ATCC, DSMZ) for confirmation. This ensures that the cell line is authentic and suitable for experiments, clinical trials, or bioproduction.
- Test Code
- 6269
- CPT Code
- 81479
- ICD Code
- Z00.00
- Price
- ₹6,500
- Sample Type
- Extracted DNA, Cell Pellet, FTA Spot, or Tissue/Tumor
- Result Time
- Reports are typically available within 10-12 days after sample receipt.
- Fasting Required
- No
- Method
- STR DNA Technology
Sample Collection
Follow the specific sample preparation instructions provided. For extracted DNA, ensure it is eluted in low TE buffer or nuclease-free water at a concentration of 10-100 ng/µl. For cell pellets, provide at least 1x10^6 cells/ml. For FTA spots, follow standard guidelines and spot 20 µl of sample. For tissue/tumor, provide approximately 5 mg.
Method: Home collection or lab visit
Laboratory Analysis
Samples should be collected in sterile, DNase-free containers. For FTA cards, use a single card per sample and pack in a separate zip bag. Ensure proper labeling with sample ID and date.
Report Delivery
Transport samples according to the specified conditions: extracted DNA and cell pellets should be frozen at -20°C and -80°C respectively, and shipped with ice packs or dry ice. FTA cards can be transported at room temperature. Tissue samples should be transported with ice packs.
Timeline: Reports are typically available within 10-12 days after sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the Cell Line Authentication DNA Test is to verify the identity and purity of cell lines used in research and clinical applications. It detects misidentification, cross-contamination, and genetic alterations that may occur during cell culture. The test uses STR profiling to generate a unique DNA fingerprint of the cell line, which is then compared to reference databases (ATCC, DSMZ) for confirmation. This ensures that the cell line is authentic and suitable for experiments, clinical trials, or bioproduction.
How to Prepare
- Extracted DNA: Elute in low TE buffer or nuclease-free water, 10-100 ng/µl, volume 10-20 µl. Freeze at -20°C and transport with ice pack.
- Cell Pellets: Provide 1x10^6 cells/ml. Freeze at -80°C and pack with dry ice.
- FTA Spot: Follow standard guidelines, spot 20 µl sample on the card. Pack single FTA card in a single zip bag.
- Tissue/Tumor: Size approx 5 mg. Transport with ice pack.
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"Cell line authentication is critical to prevent scientific misconduct and ensure reproducibility. STR profiling is the gold standard method recommended by international guidelines."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient quantity of DNA (<10 ng/µl) or cells (<1x10^6 cells/ml)
- Hemolyzed or degraded samples
- Improper labeling or documentation
- Samples not transported under required temperature conditions
Understanding Your Results
Match with reference profile
Cell line is authentic and suitable for research use.
No match with any reference profile
Cell line may be misidentified or novel; further investigation required.
Mixed profile (multiple alleles at multiple loci)
Indicates cross-contamination with another cell line; subcloning or re-authentication needed.
If you are a researcher or clinician and have concerns about the authenticity of your cell lines, or if you require guidance on interpreting the results, consult with a qualified geneticist or research director.
Limitations
- ⚠Test cannot detect all types of genetic mutations
- ⚠Reference database may not include all cell lines
- ⚠Results depend on sample quality and quantity
- ⚠Not intended for diagnostic or therapeutic use
Risks & Considerations
- ●No significant risks associated with sample collection
- ●Potential for inaccurate results if sample quality is poor
Interfering Factors
- ●Degraded DNA due to improper storage or transport
- ●Insufficient quantity of DNA or cells
- ●Contamination with other cell lines during sample collection
- ●PCR inhibitors in the sample
Compare With Similar Tests
| Test | Cell Line Authentication DNA Test | |||
|---|---|---|---|---|
| Comparison | Cell Line Authentication DNA Test |
Frequently Asked Questions
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