Cortisol Free Serum Test
Also known as: Free Cortisol Test, Serum Cortisol Free Test
Cortisol Free Serum Test test available at DNA Labs India for ₹15,000. Uses Chromatography/Mass spectrometry on Serum samples. Results in Sample must be submitted by the 7th of the month; reports are typically available after 2-3 weeks via online portal, email, or WhatsApp.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
The purpose of the Cortisol Free Serum Test is to quantify free cortisol levels in the blood to aid in the diagnosis and monitoring of adrenal gland disorders, including Cushing's syndrome and Addison's disease. It helps identify hormonal imbalances that can lead to symptoms like hypertension, diabetes, and metabolic disturbances, enabling timely medical intervention.
- Test Code
- 351
- Price
- ₹15,000
- Sample Type
- Serum
- Result Time
- Sample must be submitted by the 7th of the month; reports are typically available after 2-3 weeks via online portal, email, or WhatsApp.
- Fasting Required
- No
- Method
- Chromatography/Mass spectrometry
Sample Collection
Ensure to complete and submit the mandatory Test Send Out Consent Form (Form 35). Avoid stress and strenuous activity if possible, but no specific fasting is required unless advised by your doctor.
Method: Venipuncture
Laboratory Analysis
A healthcare professional will draw a blood sample from a vein in your arm using a standard venipuncture procedure. The process is quick and minimally invasive.
Report Delivery
Apply pressure to the puncture site with a cotton ball or bandage to prevent bleeding. Resume normal activities immediately, but avoid heavy lifting with the arm for a short period.
Timeline: Sample must be submitted by the 7th of the month; reports are typically available after 2-3 weeks via online portal, email, or WhatsApp.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the Cortisol Free Serum Test is to quantify free cortisol levels in the blood to aid in the diagnosis and monitoring of adrenal gland disorders, including Cushing's syndrome and Addison's disease. It helps identify hormonal imbalances that can lead to symptoms like hypertension, diabetes, and metabolic disturbances, enabling timely medical intervention.
How to Prepare
- Use 1 Red Top (No Additive) tube for sample collection
- Do not use SST gel barrier tubes
- Ship the sample refrigerated or frozen to maintain stability
- Duly filled Test Send Out Consent Form (Form 35) is mandatory
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"Early detection through cortisol testing is vital for managing adrenal disorders like Cushing's syndrome and Addison's disease, helping to prevent complications such as hypertension and diabetes."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Incorrect tube type (e.g., SST gel barrier tubes)
- Insufficient sample volume (less than 1 mL)
- Missing or incomplete consent form
- Sample not stored or shipped correctly
Understanding Your Results
May indicate Cushing's syndrome, adrenal tumors, pituitary disorders, or chronic stress. Further tests like imaging or ACTH stimulation may be recommended.
Level: Elevated Cortisol
May suggest Addison's disease, adrenal insufficiency, or pituitary dysfunction. Additional evaluation with ACTH tests could be necessary.
Level: Low Cortisol
Levels within reference range generally indicate normal adrenal function, but clinical correlation is essential as cortisol fluctuates diurnally.
Level: Normal Cortisol
Consult an endocrinologist if you experience symptoms of high or low cortisol, or if your test results are abnormal. Also seek medical advice if you have a history of adrenal or pituitary disorders.
Limitations
- ⚠Results may vary based on time of day and individual physiology
- ⚠Does not specify the cause of cortisol abnormality; further tests may be needed
- ⚠Not suitable for diagnosing all adrenal disorders without clinical correlation
Risks & Considerations
- ●Minor bruising or pain at the puncture site
- ●Rare risk of infection or excessive bleeding
- ●Fainting or dizziness in some individuals
Interfering Factors
- ●Stress or anxiety before the test
- ●Medications such as corticosteroids or oral contraceptives
- ●Recent surgery, illness, or vigorous exercise
- ●Pregnancy or menstrual cycle variations
Compare With Similar Tests
| Test | Cortisol Free Serum Test | Cortisol Total Test | 24-Hour Urine Cortisol Test |
|---|---|---|---|
| Comparison | Cortisol Free Serum Test |
Frequently Asked Questions
What is the Cortisol Free Serum Test?
Why is this test recommended?
How is the test performed?
What are the symptoms of high cortisol levels?
How much does the Cortisol Free Serum Test cost in India?
Is fasting required for this test?
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What is the sample type for this test?
Are there any risks associated with the test?
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