FISH - Equivocal HER2 (ERBB2) Test
Short Name: FISH HER2 Test
Also known as: HER2 FISH Test, Equivocal HER2 Gene Amplification Test
FISH - Equivocal HER2 (ERBB2) Test test available at DNA Labs India for ₹12,870. Uses FISH on Formalin fixed paraffin embedded tissue block samples. Results in Approximately 1 week from sample receipt to report generation.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of this FISH test is to clarify the HER2 status in breast cancer patients with equivocal initial results. Accurate HER2 determination is essential for identifying patients who may benefit from HER2-targeted therapies, thereby personalizing treatment and improving outcomes.
- Test Code
- 22
- Price
- ₹12,870
- Sample Type
- Formalin fixed paraffin embedded tissue block
- Result Time
- Approximately 1 week from sample receipt to report generation.
- Fasting Required
- No
- Method
- FISH
Sample Collection
Ensure all required forms, including Chromosome & FISH analysis Requisition Form (Form 17), are duly filled and submitted.
Method: Biopsy or Surgery
Laboratory Analysis
Tissue sample is collected via biopsy or surgical procedure.
Report Delivery
Sample is processed and analyzed using fluorescence in situ hybridization method.
Timeline: Approximately 1 week from sample receipt to report generation.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of this FISH test is to clarify the HER2 status in breast cancer patients with equivocal initial results. Accurate HER2 determination is essential for identifying patients who may benefit from HER2-targeted therapies, thereby personalizing treatment and improving outcomes.
How to Prepare
- Submit formalin fixed paraffin embedded tissue block
- Ship at room temperature
- Tissue block must contain at least 10% tumor tissue
- Submit duly filled Chromosome & FISH analysis Requisition Form (Form 17)
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This FISH test is crucial for resolving equivocal HER2 status, which directly impacts treatment decisions in breast cancer."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient tumor tissue in sample (<10%)
- Improperly filled or missing requisition form
- Degraded or inadequate tissue sample
Understanding Your Results
HER2 Amplified
Positive for HER2 gene amplification, suggesting eligibility for HER2-targeted therapies.
Action: Consult oncologist for personalized treatment options.
HER2 Non-Amplified
Negative for HER2 gene amplification.
Action: Discuss alternative treatment approaches with physician.
Equivocal
Borderline results; further testing may be required for definitive conclusion.
Action: Repeat test or use alternative probe as per ASCO/CAP guidelines.
If symptoms of breast cancer persist, if test results are equivocal, or to discuss treatment plans based on HER2 status.
Limitations
- ⚠Only applicable for equivocal cases from initial HER2 testing
- ⚠Requires formalin-fixed paraffin-embedded tissue sample
- ⚠Cannot be performed on blood or other non-tissue samples
- ⚠Results may be affected by tumor heterogeneity
Risks & Considerations
- ●Minimal risk associated with tissue sample collection (e.g., biopsy)
- ●Possible minor bleeding or infection at collection site
- ●Discomfort during the biopsy or surgical procedure
Interfering Factors
- ●Poor tissue fixation or processing
- ●Insufficient tumor cell content (<10%)
- ●Tissue degradation or contamination
- ●Use of non-standardized probes or reagents
Compare With Similar Tests
| Test | FISH - Equivocal HER2 (ERBB2) Test | IHC HER2 Test | SISH Test | CISH Test |
|---|---|---|---|---|
| Comparison | FISH - Equivocal HER2 (ERBB2) Test |
Frequently Asked Questions
What is the FISH - Equivocal HER2 (ERBB2) Test?
Why is this test recommended?
What does equivocal HER2 status mean?
How is the FISH test performed?
What sample is needed for this test?
Is any preparation required before the test?
How long does it take to get the results?
What is the cost of the test?
Is home sample collection available?
Are there any risks associated with the test?
How accurate is the FISH test for HER2?
What should I do after receiving the test results?
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