Fungal ITS2 Amplicon Sequencing and Analysis Test
Short Name: Fungal ITS2 Sequencing
Also known as: ITS2 Sequencing, Fungal ITS2 Amplicon Sequencing, Fungal DNA Sequencing
Fungal ITS2 Amplicon Sequencing and Analysis Test test available at DNA Labs India for ₹10,000. Uses ITS2 PCR Amplification, Next-Generation Sequencing, Bioinformatics Analysis on Extracted DNA samples. Results in Results are typically available within 8 weeks after the sample is received by the laboratory.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of Fungal ITS2 Amplicon Sequencing is to identify the exact fungal species present in a clinical sample. This is essential for guiding antifungal therapy, as different species have varying susceptibility to antifungal agents. It also helps in epidemiological studies and outbreak investigations. The test is particularly valuable for immunocompromised patients, where fungal infections can be life-threatening and require prompt, targeted treatment.
- Test Code
- 6334
- CPT Code
- 81479
- ICD Code
- B35.9
- Price
- ₹10,000
- Sample Type
- Extracted DNA
- Result Time
- Results are typically available within 8 weeks after the sample is received by the laboratory.
- Fasting Required
- No
- Method
- ITS2 PCR Amplification, Next-Generation Sequencing, Bioinformatics Analysis
Sample Collection
No special preparation is required. However, inform your doctor about any antifungal medications you are taking, as they may affect the test results.
Method: Blood or tissue sample as per clinical indication
Laboratory Analysis
Sample collection is performed by a trained phlebotomist. For blood samples, a standard venipuncture is done. For tissue or other samples, the procedure will be as per the clinical indication.
Report Delivery
No specific aftercare is needed. You can resume normal activities immediately.
Timeline: Results are typically available within 8 weeks after the sample is received by the laboratory.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of Fungal ITS2 Amplicon Sequencing is to identify the exact fungal species present in a clinical sample. This is essential for guiding antifungal therapy, as different species have varying susceptibility to antifungal agents. It also helps in epidemiological studies and outbreak investigations. The test is particularly valuable for immunocompromised patients, where fungal infections can be life-threatening and require prompt, targeted treatment.
How to Prepare
- Ensure the sample is collected in a sterile container
- Label the sample with patient ID and date of collection
- Transport the sample to the lab within 24 hours at 2-8°C
- For extracted DNA, store at -20°C until shipment
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Fungal ITS2 sequencing is a powerful tool for identifying clinically significant fungi, especially when conventional methods are inconclusive. Early and accurate diagnosis is crucial for effective antifungal therapy."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not labeled correctly
- Sample hemolyzed or clotted
- Insufficient quantity of DNA
- Sample received after prolonged delay without proper storage
Understanding Your Results
No fungal DNA detected
Fungal infection is unlikely, but clinical correlation is advised.
Specific fungal species identified
Confirms fungal infection; guides antifungal therapy.
Fungal DNA detected but species not identified
May indicate a rare or novel species; further testing may be needed.
Consult your doctor if you experience symptoms such as persistent fever, cough, skin lesions, or any signs of infection, especially if you are immunocompromised. Early diagnosis and treatment are crucial.
Limitations
- ⚠Test does not differentiate between viable and non-viable organisms
- ⚠May not detect all fungal species due to incomplete reference databases
- ⚠Requires sufficient DNA quantity and quality
- ⚠Results should be correlated with clinical findings and other diagnostic tests
- ⚠Not a substitute for culture and susceptibility testing
Risks & Considerations
- ●Minimal risk of bleeding or bruising at the needle site
- ●Infection at the biopsy site (rare)
- ●No radiation exposure
Interfering Factors
- ●Contamination with non-target fungal DNA during sample collection or processing
- ●Degraded DNA due to improper storage or transport
- ●Inhibitors in the sample (e.g., heme, polysaccharides) affecting PCR
- ●Low fungal load below detection limit
- ●Mixed fungal infections may complicate interpretation
Compare With Similar Tests
| Test | Fungal ITS2 Amplicon Sequencing and Analysis | Fungal Culture | KOH Mount | Galactomannan Assay | Panfungal PCR |
|---|---|---|---|---|---|
| Comparison | Fungal ITS2 Amplicon Sequencing and Analysis |
Frequently Asked Questions
What is Fungal ITS2 Amplicon Sequencing?
Why is ITS2 region used for fungal identification?
What sample is required for this test?
How long does the test take?
Is fasting required before the test?
Can this test detect all types of fungi?
Is home sample collection available?
What is the cost of the test?
How should I interpret a positive result?
Are there any risks associated with the test?
Can I take antifungal medications before the test?
How do I book this test?
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