Fungal ITS2 Amplicon Sequencing Data Analysis Test
Short Name: Fungal ITS2 Sequencing
Also known as: ITS2 Amplicon Sequencing, Fungal DNA Sequencing, ITS2 Sequencing Analysis
Fungal ITS2 Amplicon Sequencing Data Analysis Test test available at DNA Labs India for ₹5,000. Uses ITS2 region PCR amplification, Next-Generation Sequencing (NGS), Bioinformatics analysis on Extracted DNA samples. Results in Reports are typically available within 4 weeks from the date of sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of Fungal ITS2 Amplicon Sequencing Data Analysis is to accurately identify fungal species present in a clinical sample. This information is essential for diagnosing fungal infections, determining appropriate antifungal therapy, and monitoring treatment response. It is particularly valuable when conventional methods (culture, microscopy) are inconclusive or negative, or when a rapid and precise identification is required for optimal patient management.
- Test Code
- 6378
- CPT Code
- N/A
- ICD Code
- B35-B49
- Price
- ₹5,000
- Sample Type
- Extracted DNA
- Result Time
- Reports are typically available within 4 weeks from the date of sample receipt.
- Fasting Required
- No
- Method
- ITS2 region PCR amplification, Next-Generation Sequencing (NGS), Bioinformatics analysis
Sample Collection
No special preparation is required. However, ensure that the extracted DNA sample is of high quality and stored appropriately (e.g., -20°C) before shipping.
Method: DNA extraction from clinical specimen (e.g., tissue, swab, body fluid) performed at lab
Laboratory Analysis
The sample (extracted DNA) should be transported in a sterile, labeled tube with proper cold chain maintenance.
Report Delivery
No specific aftercare is needed. The laboratory will process the sample and provide the report.
Timeline: Reports are typically available within 4 weeks from the date of sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of Fungal ITS2 Amplicon Sequencing Data Analysis is to accurately identify fungal species present in a clinical sample. This information is essential for diagnosing fungal infections, determining appropriate antifungal therapy, and monitoring treatment response. It is particularly valuable when conventional methods (culture, microscopy) are inconclusive or negative, or when a rapid and precise identification is required for optimal patient management.
How to Prepare
- Provide the extracted DNA sample in a sterile microcentrifuge tube.
- Label the tube with patient ID and date of extraction.
- Ship the sample in a cold pack to maintain DNA integrity.
- Include the requisition form with relevant clinical history.
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Fungal ITS2 sequencing is a powerful tool for identifying clinically relevant fungi, especially in immunocompromised patients. Accurate species identification guides targeted antifungal therapy and improves patient outcomes."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample without proper labeling
- Insufficient DNA quantity (<10 ng/µL)
- Degraded DNA (e.g., visible smearing on gel)
- Sample not shipped with cold chain
- Hemolyzed or contaminated samples
Understanding Your Results
Likely causative agent of infection; guide antifungal therapy accordingly.
Possible mixed infection; consider clinical significance of each species.
Negative result; may indicate absence of fungal infection or insufficient sample.
Inconclusive; consider repeat testing with a new sample.
Consult your healthcare provider if you have symptoms of a fungal infection (e.g., persistent skin rash, nail changes, respiratory symptoms, fever of unknown origin) or if you have been advised to undergo this test. Early diagnosis and treatment are crucial.
Limitations
- ⚠Test is performed on extracted DNA; clinical specimen collection and DNA extraction are not included in this service
- ⚠Results are qualitative (presence/absence) and do not provide quantitative load
- ⚠May not distinguish between active infection and colonization
- ⚠Requires adequate DNA quality and quantity; poor samples may yield inconclusive results
- ⚠Turnaround time is 4 weeks, which may be longer than some clinical needs
Risks & Considerations
- ●No significant risks are associated with this test as it is performed on extracted DNA.
- ●Potential for inconclusive results due to sample quality issues.
Interfering Factors
- ●Contamination with non-target fungal DNA during sample processing
- ●Degraded DNA due to improper storage or handling
- ●Insufficient DNA quantity or quality
- ●Mixed infections may complicate interpretation
- ●Bioinformatics analysis may miss novel or rare species if not in database
Compare With Similar Tests
| Test | Fungal ITS2 Amplicon Sequencing Data Analysis | Fungal Culture | Microscopy (KOH mount) | Pan-fungal PCR |
|---|---|---|---|---|
| Comparison | Fungal ITS2 Amplicon Sequencing Data Analysis | Culture is traditional but slow (weeks) and may miss fastidious fungi. ITS2 sequencing is faster and more sensitive. | Microscopy is rapid but has low sensitivity and cannot identify species. ITS2 sequencing provides definitive identification. | Pan-fungal PCR detects fungal DNA but may not identify species. ITS2 sequencing provides species-level resolution. |
Frequently Asked Questions
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