Human Rotavirus Viral Load Quantitative Test
Short Name: Rotavirus Viral Load
Also known as: Rotavirus PCR Quantitative, Human Rotavirus RNA Quantification
Human Rotavirus Viral Load Quantitative Test test available at DNA Labs India for ₹10,200. Uses Real-Time PCR on Stool samples. Results in Reports are typically available on the 4th working day after sample collection. Email reports are sent within 48 hours of result availability, and phone updates within 36 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The primary purpose of the Human Rotavirus Viral Load Quantitative Test is to detect the presence of rotavirus and quantify the viral load in the stool. This aids in: 1) Confirming the diagnosis of rotavirus infection in patients with acute gastroenteritis. 2) Assessing the severity of infection based on viral load levels. 3) Monitoring the response to treatment or the natural resolution of infection. 4) Identifying rotavirus as the causative agent in outbreaks. 5) Guiding infection control measures to prevent spread. 6) Evaluating immunocompromised patients who may have prolonged or severe infections. 7) Differentiating rotavirus from other causes of diarrhea, such as bacteria or parasites.
- Test Code
- 6155
- CPT Code
- 87529
- ICD Code
- A08.0
- Price
- ₹10,200
- Sample Type
- Stool
- Result Time
- Reports are typically available on the 4th working day after sample collection. Email reports are sent within 48 hours of result availability, and phone updates within 36 hours.
- Fasting Required
- No
- Method
- Real-Time PCR
Sample Collection
No special preparation is required. However, it is important to inform the healthcare provider about any recent rotavirus vaccination or current medications. The patient must sign a consent document and provide relevant clinical history.
Method: Stool collection in sterile container
Laboratory Analysis
A stool sample is collected in a sterile, leak-proof container. For infants, a clean diaper can be used, and the stool is transferred to the container. Ensure the sample does not come into contact with urine or water.
Report Delivery
The sample should be transported to the laboratory as soon as possible. If there is a delay, the sample should be refrigerated (2-8°C) for up to 24 hours. Do not freeze the sample unless instructed.
Timeline: Reports are typically available on the 4th working day after sample collection. Email reports are sent within 48 hours of result availability, and phone updates within 36 hours.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the Human Rotavirus Viral Load Quantitative Test is to detect the presence of rotavirus and quantify the viral load in the stool. This aids in: 1) Confirming the diagnosis of rotavirus infection in patients with acute gastroenteritis. 2) Assessing the severity of infection based on viral load levels. 3) Monitoring the response to treatment or the natural resolution of infection. 4) Identifying rotavirus as the causative agent in outbreaks. 5) Guiding infection control measures to prevent spread. 6) Evaluating immunocompromised patients who may have prolonged or severe infections. 7) Differentiating rotavirus from other causes of diarrhea, such as bacteria or parasites.
How to Prepare
- Collect stool directly into the sterile container.
- Avoid mixing with urine or toilet water.
- Fill the container to the indicated line (about 5-10 g or 5 mL).
- Tightly close the lid and label with patient name and date/time of collection.
- Transport to the lab within 2 hours if possible; otherwise, refrigerate.
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Quantitative rotavirus viral load helps assess disease severity and monitor response to therapy, especially in immunocompromised patients."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample received in inappropriate container (e.g., non-sterile, leaking)
- Sample contaminated with urine or water
- Insufficient quantity of stool
- Sample delayed beyond stability period without proper storage
Understanding Your Results
Not detected
No rotavirus RNA detected. Active infection is unlikely. However, if symptoms persist, consider other pathogens or repeat testing.
Detected (low viral load, e.g., <10^4 copies/mL)
Indicates active rotavirus infection. Low viral load may be seen in early or resolving infection. Clinical correlation is advised.
Detected (moderate to high viral load, e.g., >10^4 copies/mL)
Suggests active and potentially severe infection. Higher viral loads may correlate with more pronounced symptoms and higher risk of transmission.
Consult a physician immediately if you or your child experience signs of dehydration, such as dry mouth, decreased urination, lethargy, or sunken eyes. Also seek medical advice if diarrhea persists for more than 3 days, if there is blood in the stool, or if there is high fever.
Limitations
- ⚠A negative result does not completely rule out rotavirus infection if the sample was collected late in the course of illness.
- ⚠Viral load may not directly correlate with clinical severity in all patients.
- ⚠The test cannot differentiate between vaccine strain and wild-type rotavirus.
- ⚠Quantitative results should be interpreted in conjunction with clinical findings.
Risks & Considerations
- ●No significant risks are associated with stool sample collection.
- ●In rare cases, patients may feel discomfort during sample collection, but it is generally safe.
Interfering Factors
- ●Improper stool collection or storage may degrade viral RNA, leading to false negatives.
- ●Recent rotavirus vaccination may cause a positive result due to live attenuated vaccine virus.
- ●Inhibitors in stool (e.g., bile salts, polysaccharides) can interfere with PCR amplification.
- ●Sample contamination with urine or water can dilute the viral load.
Compare With Similar Tests
| Test | Human Rotavirus Viral Load Quantitative Test | Rotavirus Antigen Test | Rotavirus Culture | Rotavirus PCR (Qualitative) |
|---|---|---|---|---|
| Comparison | Human Rotavirus Viral Load Quantitative Test |
Frequently Asked Questions
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