Immunophenotyping by Flow Cytometry CD25 Test
Short Name: CD25 Test by Flow Cytometry
Also known as: CD25 Test, IL2RA Test, Interleukin-2 Receptor Alpha Test
Immunophenotyping by Flow Cytometry CD25 Test test available at DNA Labs India for ₹3,500. Uses Flow Cytometry on 3 mL whole blood in Lavender Top (EDTA) tube and Green Top (Sodium Heparin) tube, or 2 mL bone marrow in Green Top (Sodium heparin) tube samples. Results in Same day report for samples received by 9 AM; otherwise, report may be delayed.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The purpose of the CD25 test is to diagnose and monitor diseases involving immune dysregulation, particularly cancers such as leukemia and lymphoma, autoimmune conditions like rheumatoid arthritis, and immunodeficiency disorders. It aids in identifying abnormal cell populations and assessing immune response.
- Test Code
- 1060
- Price
- ₹3,500
- Sample Type
- 3 mL whole blood in Lavender Top (EDTA) tube and Green Top (Sodium Heparin) tube, or 2 mL bone marrow in Green Top (Sodium heparin) tube
- Result Time
- Same day report for samples received by 9 AM; otherwise, report may be delayed.
- Fasting Required
- No
- Method
- Flow Cytometry
Sample Collection
No special preparation required. Provide brief clinical history and inform about any medications or medical conditions.
Method: Venipuncture or Bone Marrow Aspiration
Laboratory Analysis
A trained healthcare professional will collect a blood sample via venipuncture or perform a bone marrow aspiration under sterile conditions.
Report Delivery
Apply gentle pressure to the puncture site to prevent bruising. No major post-test restrictions; resume normal activities unless advised otherwise.
Timeline: Same day report for samples received by 9 AM; otherwise, report may be delayed.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the CD25 test is to diagnose and monitor diseases involving immune dysregulation, particularly cancers such as leukemia and lymphoma, autoimmune conditions like rheumatoid arthritis, and immunodeficiency disorders. It aids in identifying abnormal cell populations and assessing immune response.
How to Prepare
- Ship sample immediately at 18-22°C
- Do not refrigerate or freeze the sample
- Specify time, date, and clinical details on the test request form
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"The CD25 test is vital for assessing immune cell abnormalities in cancer patients, aiding in diagnosis and treatment planning."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient sample volume
- Incorrect sample container or anticoagulant
- Sample transported at incorrect temperature or delayed beyond stability period
Understanding Your Results
Elevated CD25+ lymphocytes
Low or absent CD25+ lymphocytes
Normal CD25 expression
Consult a doctor if you experience symptoms such as unexplained fever, fatigue, weight loss, night sweats, joint pain, enlarged lymph nodes, or recurrent infections, or if test results are abnormal.
Limitations
- ⚠Results are not specific to a single disease; clinical correlation required
- ⚠Requires expert interpretation and specialized equipment
- ⚠Dependent on sample quality and handling
Risks & Considerations
- ●Minimal risks from blood draw: bruising, pain, rare infection at puncture site
- ●Bone marrow aspiration carries higher risks: bleeding, infection, discomfort
Interfering Factors
- ●Use of immunosuppressive or biologic therapies
- ●Recent infections or vaccinations
- ●Sample hemolysis, improper storage, or delayed processing
Compare With Similar Tests
| Test | Immunophenotyping by Flow Cytometry CD25 Test | Flow Cytometry Panel | CD3 Test | Complete Blood Count (CBC) |
|---|---|---|---|---|
| Comparison | Immunophenotyping by Flow Cytometry CD25 Test |
Frequently Asked Questions
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