Immunophenotyping by Flow Cytometry ZAP-70 Test
Short Name: ZAP-70 Flow Cytometry
Also known as: ZAP-70 Flow Cytometry, ZAP-70 Immunophenotyping, ZAP-70 Protein Expression Test
Immunophenotyping by Flow Cytometry ZAP-70 Test test available at DNA Labs India for ₹3,500. Uses Flow Cytometry on Whole blood or bone marrow samples. Results in Sample daily by 9 am; report same day. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The purpose is to detect ZAP-70 expression by flow cytometry on leukemic B cells to aid in the diagnosis and prognostic assessment of chronic lymphocytic leukemia (CLL).
- Test Code
- 3498
- Price
- ₹3,500
- Sample Type
- Whole blood or bone marrow
- Result Time
- Sample daily by 9 am; report same day
- Fasting Required
- No
- Method
- Flow Cytometry
Sample Collection
Give a brief clinical history including time, date, and relevant clinical details on the test request form. No fasting is required.
Method: Venipuncture for whole blood; bone marrow aspiration for bone marrow sample
Laboratory Analysis
Whole blood is collected in one EDTA tube and one sodium heparin tube. Bone marrow is collected in a sodium heparin tube by a trained clinician.
Report Delivery
Ship immediately at 18–22°C. Do not refrigerate or freeze.
Timeline: Sample daily by 9 am; report same day
Patient Instructions
About This Test
Who Should Get This Test
The purpose is to detect ZAP-70 expression by flow cytometry on leukemic B cells to aid in the diagnosis and prognostic assessment of chronic lymphocytic leukemia (CLL).
How to Prepare
- Collect 3 mL whole blood in one Lavender Top (EDTA) tube and 3 mL whole blood in one Green Top (Sodium Heparin) tube; alternatively collect 2 mL bone marrow in one Green Top (Sodium Heparin) tube.
- Label samples with patient identification and collection time.
- Ship immediately at 18–22°C. Do not refrigerate or freeze.
- Write time, date, and clinical details on the test request form.
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"ZAP-70 status by flow cytometry is a key prognostic marker in chronic lymphocytic leukemia. A positive ZAP-70 result is associated with more aggressive disease and may influence treatment planning. Always interpret this result with clinical findings, complete blood counts, morphology, cytogenetic markers, and other immunophenotypic findings."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Frozen or refrigerated samples
- Samples older than 24 hours
- Clotted, hemolyzed, or insufficient volume samples
- Unlabeled or mismatched samples
Understanding Your Results
If you have symptoms such as unexplained weight loss, fever, night sweats, fatigue, swollen lymph nodes, easy bleeding or bruising, or abnormal blood counts, consult a hematologist or oncologist.
Limitations
- ⚠ZAP-70 testing requires fresh, viable cells and should be performed promptly after collection
- ⚠Flow cytometry gating and antibody panels may vary by laboratory
- ⚠ZAP-70 alone is not diagnostic; results should be correlated with morphology, cytogenetics, immunophenotype, and clinical findings
Risks & Considerations
- ●Mild pain or bruising at venipuncture site
- ●Bone marrow aspiration may cause temporary discomfort or soreness
Interfering Factors
- ●Improper sample storage or temperature during transport may reduce cell viability and affect results
- ●Recent chemotherapy or immunotherapy can alter cell populations and ZAP-70 expression
- ●Hemodiluted, clotted, or insufficient samples may yield unreliable results
Compare With Similar Tests
| Test | Immunophenotyping by Flow Cytometry ZAP-70 Test | ||||
|---|---|---|---|---|---|
| Comparison | Immunophenotyping by Flow Cytometry ZAP-70 Test |
Frequently Asked Questions
What is the ZAP-70 test?
Why is ZAP-70 testing done in CLL?
What sample is required for the ZAP-70 test?
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