JEV (RNA Detection) Qualitative Test
Short Name: JEV RNA Qualitative
Also known as: Japanese Encephalitis Virus RNA PCR, JEV RT-PCR
JEV (RNA Detection) Qualitative Test test available at DNA Labs India for ₹6,800. Uses Real Time PCR on Whole Blood, Serum, Plasma, C.S.F samples. Results in Reports are available on the 3rd working day after sample collection. Email reports within 36 hours, phone support 24 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of the JEV (RNA Detection) Qualitative Test is to confirm or rule out the presence of Japanese Encephalitis Virus RNA in patients presenting with symptoms suggestive of viral encephalitis, especially in endemic areas. Early detection allows for prompt supportive care, antiviral therapy (if applicable), and implementation of preventive measures to reduce transmission. The test is also useful for epidemiological surveillance and outbreak investigations.
- Test Code
- 6167
- CPT Code
- 87798
- ICD Code
- A83.0
- Price
- ₹6,800
- Sample Type
- Whole Blood, Serum, Plasma, C.S.F
- Result Time
- Reports are available on the 3rd working day after sample collection. Email reports within 36 hours, phone support 24 hours.
- Fasting Required
- No
- Method
- Real Time PCR
Sample Collection
No special preparation is required. However, patients must sign a consent document and provide relevant clinical history.
Method: Venipuncture or Lumbar Puncture
Laboratory Analysis
Blood sample is collected via venipuncture; CSF sample is collected by a healthcare professional via lumbar puncture.
Report Delivery
Sample should be transported to the laboratory immediately. No specific post-collection precautions are needed for the patient.
Timeline: Reports are available on the 3rd working day after sample collection. Email reports within 36 hours, phone support 24 hours.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the JEV (RNA Detection) Qualitative Test is to confirm or rule out the presence of Japanese Encephalitis Virus RNA in patients presenting with symptoms suggestive of viral encephalitis, especially in endemic areas. Early detection allows for prompt supportive care, antiviral therapy (if applicable), and implementation of preventive measures to reduce transmission. The test is also useful for epidemiological surveillance and outbreak investigations.
How to Prepare
- Use sterile collection tubes (EDTA for blood, sterile container for CSF)
- Label the sample with patient ID and date/time of collection
- Transport sample to laboratory at 2-8°C if processing within 24 hours; otherwise freeze at -20°C
- Avoid repeated freeze-thaw cycles
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"Early detection of JEV RNA is critical for timely management of Japanese encephalitis, especially in endemic regions. This qualitative RT-PCR test offers high sensitivity and specificity, aiding in prompt diagnosis and treatment decisions."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or lipemic samples
- Improperly labeled samples
- Samples received in inappropriate containers
- Samples with prolonged transit time without proper storage
Understanding Your Results
Positive
JEV RNA detected. Consistent with acute Japanese encephalitis infection. Clinical correlation and further management advised.
Negative
JEV RNA not detected. Does not rule out infection; consider repeat testing or alternative diagnosis.
Consult a physician immediately if you experience symptoms such as high fever, severe headache, stiff neck, confusion, seizures, or coma, especially after travel to endemic areas.
Limitations
- ⚠Qualitative test does not quantify viral load
- ⚠Negative result does not exclude JEV infection if sample collected too early or late
- ⚠Test may not detect other encephalitis-causing viruses
- ⚠Requires specialized laboratory equipment and trained personnel
Risks & Considerations
- ●Minimal risk of bleeding or bruising at venipuncture site
- ●CSF collection carries rare risks like headache or infection
Interfering Factors
- ●Hemolysis of blood sample may affect RNA stability
- ●Improper storage or delayed processing can degrade RNA
- ●Contamination during sample collection or handling
- ●Low viral load may yield false-negative results
- ●Cross-reactivity with other flaviviruses (rare)
Compare With Similar Tests
| Test | JEV (RNA Detection) Qualitative Test | JEV IgM ELISA | Virus Isolation | Immunohistochemistry |
|---|---|---|---|---|
| Comparison | JEV (RNA Detection) Qualitative Test | Detects antibodies, may be negative in early infection; RT-PCR detects viral RNA earlier. | Time-consuming and requires BSL-3 facilities; RT-PCR is faster and safer. | Used on tissue samples; not suitable for routine diagnosis. |
Frequently Asked Questions
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Can the test be done at home?
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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
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✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
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