Marburg, Lassa & Ebola Multiplex Detection and Differentiation (RNA Detection) Qualitative Test
Short Name: Marburg-Lassa-Ebola Multiplex PCR
Also known as: VHF Multiplex PCR, Ebola Lassa Marburg RNA Test, Hemorrhagic Fever Panel
Marburg, Lassa & Ebola Multiplex Detection and Differentiation (RNA Detection) Qualitative Test test available at DNA Labs India for ₹11,560. Uses Real Time PCR on Whole Blood, Serum, Plasma, CSF, Oral Fluid, Oral Swab, Nasal Swab, Throat Swab, Body Secretions, Organs, Other Bodily Fluids, Semen, Tears samples. Results in Reports delivered on the 4th working day. Email within 48 hours, phone within 36 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of this test is to detect and differentiate between Marburg, Lassa, and Ebola virus infections in individuals presenting with symptoms suggestive of viral hemorrhagic fever (VHF). Early identification of the specific virus helps in initiating appropriate supportive care, implementing infection control measures, and guiding public health responses. The test is qualitative, meaning it reports the presence or absence of viral RNA, not the viral load.
- Test Code
- 6191
- CPT Code
- 87529
- ICD Code
- A98.4 (Ebola), A96.2 (Lassa), A98.3 (Marburg)
- Price
- ₹11,560
- Sample Type
- Whole Blood, Serum, Plasma, CSF, Oral Fluid, Oral Swab, Nasal Swab, Throat Swab, Body Secretions, Organs, Other Bodily Fluids, Semen, Tears
- Result Time
- Reports delivered on the 4th working day. Email within 48 hours, phone within 36 hours.
- Fasting Required
- No
- Method
- Real Time PCR
Sample Collection
Patient must sign a consent document and provide any relevant clinical history. No fasting required. Inform the lab about any recent travel or exposure.
Method: Venipuncture or swab collection
Laboratory Analysis
Blood sample will be drawn by a trained phlebotomist. For swabs, a sterile swab will be used to collect samples from the specified site. The procedure is quick and minimally invasive.
Report Delivery
No special precautions needed. The sample will be transported to the laboratory immediately. Results will be available on the 4th working day.
Timeline: Reports delivered on the 4th working day. Email within 48 hours, phone within 36 hours.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this test is to detect and differentiate between Marburg, Lassa, and Ebola virus infections in individuals presenting with symptoms suggestive of viral hemorrhagic fever (VHF). Early identification of the specific virus helps in initiating appropriate supportive care, implementing infection control measures, and guiding public health responses. The test is qualitative, meaning it reports the presence or absence of viral RNA, not the viral load.
How to Prepare
- Use sterile collection tubes and swabs
- Label the sample with patient ID and date of collection
- Transport samples at 2-8°C if processing within 24 hours, otherwise freeze at -80°C
- Avoid repeated freeze-thaw cycles
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Early differentiation of viral hemorrhagic fevers is critical for patient isolation and targeted therapy. This multiplex PCR assay provides rapid, reliable results to guide clinical management."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or clotted blood samples
- Insufficient sample volume
- Samples not stored at appropriate temperature
- Unlabeled or mislabeled samples
- Samples received after prolonged delay without proper preservation
Understanding Your Results
Positive for Marburg virus infection. Immediate isolation and supportive care required.
Positive for Lassa virus infection. Early treatment with ribavirin may be considered.
Positive for Ebola virus infection. Urgent isolation and intensive care needed.
No evidence of infection with these viruses. Consider other etiologies if symptoms persist.
Consult a physician immediately if you have symptoms like fever, bleeding, or have been exposed to a suspected case. Early diagnosis and treatment are critical.
Limitations
- ⚠Qualitative test does not provide viral load quantification
- ⚠Negative result does not rule out infection if sample was collected too early or too late
- ⚠Test should be performed in BSL-3/4 facilities for safety
- ⚠Results must be correlated with clinical presentation and epidemiological history
Risks & Considerations
- ●Minimal risk of bleeding or infection at venipuncture site
- ●Discomfort during swab collection
- ●No serious risks associated with the test itself
Interfering Factors
- ●Improper sample collection or storage leading to RNA degradation
- ●Contamination during sample processing
- ●Presence of PCR inhibitors in the sample
- ●Cross-reactivity with other viral pathogens (rare)
- ●Sample collected during very early or late stages of infection may yield false negatives
Compare With Similar Tests
| Test | Marburg, Lassa & Ebola Multiplex Detection and Differentiation (RNA Detection) Qualitative Test | Ebola Zaire RT-PCR | Lassa Virus IgG/IgM ELISA | Viral Culture |
|---|---|---|---|---|
| Comparison | Marburg, Lassa & Ebola Multiplex Detection and Differentiation (RNA Detection) Qualitative Test | Targets only Ebola Zaire species, while this multiplex covers multiple VHF viruses. | Serology detects antibodies, not active infection; PCR is more sensitive in early disease. | Culture is slow and requires BSL-4 facilities; PCR is faster and safer. |
Frequently Asked Questions
What is the cost of the Marburg, Lassa & Ebola Multiplex Test?
What sample types are accepted for this test?
Is fasting required before the test?
How long does it take to get the results?
Can this test differentiate between Marburg, Lassa, and Ebola?
Is home sample collection available?
What is the sensitivity of this test?
Are there any risks associated with the test?
Do I need a doctor's prescription for this test?
What should I do if my result is positive?
Can this test be done during pregnancy?
Is the test NABL accredited?
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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
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✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
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