MERS (Middle East Respiratory Syndrome) (UpE, ORF1a & ORF1b) Multiplex Detection and Differentiation (RNA Detection) Qualitative Test
Short Name: MERS-CoV Multiplex PCR
Also known as: MERS-CoV PCR, MERS-CoV RNA Detection, MERS Multiplex Test
MERS (Middle East Respiratory Syndrome) (UpE, ORF1a & ORF1b) Multiplex Detection and Differentiation (RNA Detection) Qualitative Test test available at DNA Labs India for ₹11,560. Uses Real-Time PCR on Sputum, throat and nasal swabs in viral transport medium, lung tissues, cell cultures, respiratory swab, endotracheal aspirate, serum, stool, feces samples. Results in Reports are available on the 4th working day after sample collection. Email delivery within 48 hours, phone within 36 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of this test is to detect the presence of MERS-CoV RNA in clinical specimens, confirming active infection. It aids in the diagnosis of MERS in symptomatic patients, supports outbreak investigations, and helps differentiate MERS from other respiratory infections. The multiplex design targeting UpE, ORF1a, and ORF1b increases confidence in results, especially in early infection when viral load may be low.
- Test Code
- 6196
- CPT Code
- 87507
- ICD Code
- B97.29
- Price
- ₹11,560
- Sample Type
- Sputum, throat and nasal swabs in viral transport medium, lung tissues, cell cultures, respiratory swab, endotracheal aspirate, serum, stool, feces
- Result Time
- Reports are available on the 4th working day after sample collection. Email delivery within 48 hours, phone within 36 hours.
- Fasting Required
- No
- Method
- Real-Time PCR
Sample Collection
No special preparation is required. Inform your healthcare provider about any medications or underlying conditions. A signed consent document is required.
Method: Healthcare professional
Laboratory Analysis
For swabs, a healthcare professional will gently insert a swab into the nostril or throat. For sputum, you will be asked to cough deeply to produce phlegm. The procedure is quick and minimally invasive.
Report Delivery
No specific precautions are needed. You may resume normal activities immediately.
Timeline: Reports are available on the 4th working day after sample collection. Email delivery within 48 hours, phone within 36 hours.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this test is to detect the presence of MERS-CoV RNA in clinical specimens, confirming active infection. It aids in the diagnosis of MERS in symptomatic patients, supports outbreak investigations, and helps differentiate MERS from other respiratory infections. The multiplex design targeting UpE, ORF1a, and ORF1b increases confidence in results, especially in early infection when viral load may be low.
How to Prepare
- For respiratory swabs: use synthetic fiber swabs with plastic shafts; place in viral transport medium
- For sputum: collect in a sterile, leak-proof container; ensure it is from the lower respiratory tract
- For serum: collect in a serum separator tube; allow to clot before centrifugation
- For stool: collect a small amount in a sterile container
- Label all specimens with patient identification and collection date/time
- Transport specimens to the laboratory as soon as possible, preferably on ice
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Early detection of MERS-CoV is critical for outbreak control and patient management. This multiplex assay enhances sensitivity by targeting multiple genomic regions."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Unlabeled or mislabeled specimens
- Inappropriate transport medium
- Leaking or broken containers
- Specimens received after prolonged delay without proper refrigeration
- Insufficient sample volume
Understanding Your Results
No evidence of MERS-CoV infection. However, if clinical suspicion is high, consider repeat testing or alternative diagnostics.
MERS-CoV RNA present. Confirms active infection. Clinical correlation and public health reporting are required.
Possible early infection or low viral load. Confirmatory testing may be needed.
Consult a physician immediately if you have fever, cough, shortness of breath, and a history of travel to MERS-endemic areas or contact with a confirmed case. Early testing and supportive care are crucial.
Limitations
- ⚠A negative result does not rule out MERS-CoV infection; if clinical suspicion remains, repeat testing or alternative testing may be needed
- ⚠The test detects RNA, not viable virus; positive results do not imply infectivity
- ⚠Extrapulmonary samples (serum, stool) may have lower sensitivity compared to respiratory samples
- ⚠Results should be interpreted in conjunction with clinical presentation and epidemiological history
Risks & Considerations
- ●Minimal risk of discomfort during swab collection
- ●Rare risk of bleeding or infection at collection site
- ●No significant systemic risks
Interfering Factors
- ●Improper sample collection or storage may degrade RNA, leading to false negatives
- ●Inhibition of PCR by substances in the sample (e.g., blood, mucus)
- ●Low viral load in early or late stages of infection
- ●Cross-contamination during collection or processing
Compare With Similar Tests
| Test | MERS (Middle East Respiratory Syndrome) (UpE, ORF1a & ORF1b) Multiplex Detection and Differentiation (RNA Detection) Qualitative Test | MERS-CoV Serology (Antibody Test) | Rapid Antigen Test | Single-target MERS PCR |
|---|---|---|---|---|
| Comparison | MERS (Middle East Respiratory Syndrome) (UpE, ORF1a & ORF1b) Multiplex Detection and Differentiation (RNA Detection) Qualitative Test | Serology detects past infection (antibodies) and is not useful for early diagnosis. PCR detects active viral RNA and is preferred for acute infection. | Antigen tests are faster but less sensitive than PCR. PCR is the gold standard for MERS diagnosis. | This multiplex test targets three genes, reducing the risk of false negatives due to genetic variation, unlike single-target assays. |
Frequently Asked Questions
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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
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