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Newborn Screening Panel: NBS Quad Test

DNA Labs India | ISO 9001:2015 Certified

Newborn Screening Panel: NBS Quad Test

Also known as: NBS QUAD Test, Newborn Screening QUAD Panel

Newborn Screening Panel: NBS Quad Test test available at DNA Labs India for ₹1,170. Uses Fluoroimmunoassay on Heel prick blood on filter paper samples. Results in Reports available the next day after sample receipt.. Free home collection in 300+ cities across India.

Screening PanelNewborn🏠 Home Collection

🩺 Medically Reviewed By

Overview

The NBS Quad Test is designed to screen newborns for four critical conditions: Phenylketonuria (PKU), Cystic Fibrosis, Hypothyroidism, and Biotinidase Deficiency, enabling early intervention and treatment.

Test Code
1303
Price
₹1,170
Sample Type
Heel prick blood on filter paper
Result Time
Reports available the next day after sample receipt.
Fasting Required
No
Method
Fluoroimmunoassay
Step 1

Sample Collection

Ensure clinical details and drug history are provided. No special preparation needed for the baby.

Method: Heel prick

Step 2

Laboratory Analysis

A trained phlebotomist will perform a heel prick to collect blood drops on filter paper.

Step 3

Report Delivery

Apply a small bandage to the heel. Monitor for any signs of discomfort or infection.

Timeline: Reports available the next day after sample receipt.

Patient Instructions

1
Before the Test:No fasting required. Ensure baby is comfortable and clinical details are available.
2
During the Test:Quick heel prick procedure to collect blood samples.
3
After the Test:Baby may experience minor discomfort; apply gentle pressure if needed.

About This Test

Who Should Get This Test

The NBS Quad Test is designed to screen newborns for four critical conditions: Phenylketonuria (PKU), Cystic Fibrosis, Hypothyroidism, and Biotinidase Deficiency, enabling early intervention and treatment.

How to Prepare

  • Provide clinical details and drug history with the sample
  • Collect sample as per protocol
  • Ship refrigerated or frozen if necessary

Doctor's Notes

Reviewed by — MBBS, MD (Medical Genetics) · Reg. No. 8532

"Early newborn screening is vital for the timely diagnosis and management of metabolic disorders, ensuring better health outcomes for infants."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeHeel prick blood on filter paper
Sample Volume1 drop per spot on 3 spots
ContainerFilter paper card
Collection MethodHeel prick

Sample Stability

Room Temperature: 2 hours
Refrigerator: 1 week
Frozen: 1 week
Sample Rejection Criteria:
  • Insufficient sample volume
  • Contaminated or hemolyzed sample
  • Missing clinical information

Understanding Your Results

Results indicate the presence or absence of markers for the screened conditions. Abnormal results require confirmatory testing and specialist consultation.
📊

Elevated phenylalanine levels may indicate PKU.

📊

Abnormal immunoreactive trypsinogen (IRT) levels may suggest CF.

📊

Elevated TSH levels may indicate hypothyroidism.

📊

Low biotinidase activity may indicate BTD.

⚠️ When to Consult a Doctor:

If the test results are abnormal or if the baby shows any symptoms of the screened conditions, consult a pediatrician or geneticist immediately.

Limitations

  • Screening test, not confirmatory
  • False positives possible
  • May require follow-up testing

Risks & Considerations

  • Minor pain or discomfort from heel prick
  • Small risk of bruising or infection
  • Emotional stress for parents

Interfering Factors

  • Medications affecting hormone levels
  • Premature birth
  • Sample handling errors

Frequently Asked Questions

What is the NBS Quad Test?
The NBS Quad Test is a newborn screening panel that tests for four conditions: Phenylketonuria (PKU), Cystic Fibrosis, Hypothyroidism, and Biotinidase Deficiency.
Why is the NBS Quad Test important?
It detects conditions early, allowing for timely treatment to prevent long-term health problems like brain damage or developmental delays.
What conditions does the NBS Quad Test screen for?
It screens for Phenylketonuria (PKU), Cystic Fibrosis (CF), Hypothyroidism, and Biotinidase Deficiency (BTD).
How is the NBS Quad Test performed?
A few drops of blood are collected from the baby's heel and sent to a lab for analysis using fluoroimmunoassay.
What is the cost of the NBS Quad Test?
The cost is INR 1170.00, with free home sample collection available in many cities across India.
When should the NBS Quad Test be done?
It is recommended shortly after birth, typically within the first few days of life, as part of routine newborn screening.
What if my baby's test result is positive?
A positive result requires confirmatory testing and consultation with a specialist for diagnosis and management.
Are there any risks associated with the NBS Quad Test?
Risks are minimal, including minor discomfort from the heel prick, slight bruising, or rare infection.
How accurate is the NBS Quad Test?
It is a highly sensitive screening test, but false positives can occur; follow-up testing is essential for confirmation.
Is the test painful for the baby?
The heel prick may cause brief discomfort, but it is generally well-tolerated with minimal pain.
How long does it take to get the results?
Reports are typically available the next day after the sample is received by the laboratory.
Can the NBS Quad Test be done at home?
Yes, free home sample collection is offered for online bookings across numerous cities in India.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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Your medical data is protected under Indian law.

Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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