Parvo Virus B19 Viral Load Quantitative Test
Short Name: Parvo B19 Viral Load
Also known as: Parvovirus B19 PCR, B19V DNA Quantification, Erythema Infectiosum Viral Load
Parvo Virus B19 Viral Load Quantitative Test test available at DNA Labs India for ₹8,160. Uses Real-Time PCR on Blood (EDTA plasma) or respiratory secretions (saliva, sputum, nasal mucus) samples. Results in Reports are delivered on the 4th working day after sample collection. Email reports within 48 hours, phone consultation within 36 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The primary purpose of the Parvo Virus B19 Viral Load Quantitative Test is to confirm the presence of parvovirus B19 and determine the viral burden in the blood. This information is vital for: 1) Diagnosing acute or persistent parvovirus B19 infection, especially in patients with compatible symptoms or exposure history. 2) Monitoring immunocompromised patients (e.g., HIV, transplant recipients, chemotherapy patients) who are at risk of chronic anemia due to persistent viral replication. 3) Assessing the severity of infection in patients with hemolytic disorders (e.g., sickle cell disease, hereditary spherocytosis) who may develop transient aplastic crisis. 4) Evaluating response to antiviral therapy or immunoglobulin treatment. 5) Investigating cases of fetal anemia or hydrops fetalis in pregnant women. 6) Differentiating parvovirus B19 infection from other causes of rash, arthropathy, or anemia.
- Test Code
- 6222
- CPT Code
- 87798
- ICD Code
- B08.3
- Price
- ₹8,160
- Sample Type
- Blood (EDTA plasma) or respiratory secretions (saliva, sputum, nasal mucus)
- Result Time
- Reports are delivered on the 4th working day after sample collection. Email reports within 48 hours, phone consultation within 36 hours.
- Fasting Required
- No
- Method
- Real-Time PCR
Sample Collection
No special preparation required. Inform your healthcare provider about any medications you are taking, especially immunosuppressants or antivirals. A consent form must be signed before sample collection.
Method: Venipuncture or swab collection
Laboratory Analysis
Blood sample will be drawn from a vein in your arm using a sterile needle. If respiratory secretion is required, a swab will be taken from your nose or throat. The procedure is quick and minimally invasive.
Report Delivery
You can resume normal activities immediately. There may be slight bruising at the puncture site, which is normal. Your healthcare provider will discuss the results with you once available.
Timeline: Reports are delivered on the 4th working day after sample collection. Email reports within 48 hours, phone consultation within 36 hours.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the Parvo Virus B19 Viral Load Quantitative Test is to confirm the presence of parvovirus B19 and determine the viral burden in the blood. This information is vital for: 1) Diagnosing acute or persistent parvovirus B19 infection, especially in patients with compatible symptoms or exposure history. 2) Monitoring immunocompromised patients (e.g., HIV, transplant recipients, chemotherapy patients) who are at risk of chronic anemia due to persistent viral replication. 3) Assessing the severity of infection in patients with hemolytic disorders (e.g., sickle cell disease, hereditary spherocytosis) who may develop transient aplastic crisis. 4) Evaluating response to antiviral therapy or immunoglobulin treatment. 5) Investigating cases of fetal anemia or hydrops fetalis in pregnant women. 6) Differentiating parvovirus B19 infection from other causes of rash, arthropathy, or anemia.
How to Prepare
- Use EDTA lavender-top tube for blood collection
- For respiratory secretions, use sterile viral transport medium
- Label the sample with patient name, date, and time of collection
- Transport the sample to the laboratory within 24 hours at 2-8°C
- Do not freeze whole blood; freeze plasma if storage is required
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Quantitative parvovirus B19 PCR is essential for monitoring immunocompromised patients and assessing response to therapy. Early detection prevents severe anemia and aplastic crisis."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or clotted blood sample
- Insufficient sample volume
- Sample received in wrong container (e.g., serum separator tube)
- Sample stored at room temperature for more than 24 hours
- Improperly labeled or unlabeled sample
Understanding Your Results
No parvovirus B19 DNA detected
No active infection; however, recent infection may be below detection limit. Serology may be helpful.
Minimal viral replication
May indicate resolving infection or early infection. Monitor clinically.
Active viral replication
Consistent with acute infection. Assess for symptoms and complications.
High level of viral replication
Suggests severe infection, especially in immunocompromised or anemic patients. Urgent management required.
Consult your healthcare provider if you have symptoms of parvovirus B19 infection, especially if you are pregnant, immunocompromised, or have a blood disorder. Seek immediate medical attention if you develop severe anemia, shortness of breath, or fetal distress.
Limitations
- ⚠A negative result does not completely rule out infection if sample was collected too early or too late
- ⚠Viral load may be below the limit of detection in mild or resolving infections
- ⚠Quantitative results should be interpreted in conjunction with clinical findings and serology
- ⚠Test cannot differentiate between latent and active infection without clinical correlation
Risks & Considerations
- ●Minor bruising or bleeding at venipuncture site
- ●Dizziness or fainting during blood draw
- ●Infection (rare, if sterile technique is not followed)
Interfering Factors
- ●Hemolysis of blood sample may affect PCR accuracy
- ●Contamination with nucleases can degrade viral DNA
- ●Inhibitors in the sample (e.g., heparin, bilirubin) may reduce PCR efficiency
- ●Improper storage or delayed processing can lead to false-negative results
Compare With Similar Tests
| Test | Parvo Virus B19 Viral Load Quantitative Test | Parvovirus B19 IgM/IgG Serology | Complete Blood Count (CBC) | Bone Marrow Examination |
|---|---|---|---|---|
| Comparison | Parvo Virus B19 Viral Load Quantitative Test | Serology detects antibodies, indicating past or recent infection. PCR detects viral DNA, confirming active infection. PCR is more sensitive in immunocompromised patients who may not produce antibodies. | CBC may show anemia, reticulocytopenia, or neutropenia, but is non-specific. PCR provides definitive evidence of viral presence. | Bone marrow biopsy can show erythroid hypoplasia in aplastic crisis, but is invasive. PCR is non-invasive and more rapid. |
Frequently Asked Questions
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