Shotgun Metagenome Sequencing Data Analysis Test
Short Name: Shotgun Metagenome Analysis
Also known as: Shotgun Metagenomics, Whole Genome Metagenomic Sequencing, Metagenomic Next-Generation Sequencing (mNGS)
Shotgun Metagenome Sequencing Data Analysis Test test available at DNA Labs India for ₹18,000. Uses Shotgun Metagenomic Sequencing (Illumina platform), Bioinformatics analysis on Extracted DNA samples. Results in Reports are typically available within 4 weeks from sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
The purpose of shotgun metagenome sequencing data analysis is to identify and characterize all microorganisms present in a clinical sample by sequencing their entire genetic material. This helps in detecting pathogens that may be causing symptoms, understanding microbial diversity, and identifying antimicrobial resistance genes. It is especially useful in cases of unexplained infections, sepsis, pneumonia, meningitis, and chronic conditions where standard tests are inconclusive.
- Test Code
- 6377
- CPT Code
- N/A
- ICD Code
- N/A
- Price
- ₹18,000
- Sample Type
- Extracted DNA
- Result Time
- Reports are typically available within 4 weeks from sample receipt.
- Fasting Required
- No
- Method
- Shotgun Metagenomic Sequencing (Illumina platform), Bioinformatics analysis
Sample Collection
No special preparation required. Inform your physician about any ongoing antibiotics or medications.
Method: Blood / Tissue / Swab / Body fluid
Laboratory Analysis
Sample collection is performed by trained phlebotomists using sterile techniques.
Report Delivery
No specific precautions. Resume normal activities immediately.
Timeline: Reports are typically available within 4 weeks from sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of shotgun metagenome sequencing data analysis is to identify and characterize all microorganisms present in a clinical sample by sequencing their entire genetic material. This helps in detecting pathogens that may be causing symptoms, understanding microbial diversity, and identifying antimicrobial resistance genes. It is especially useful in cases of unexplained infections, sepsis, pneumonia, meningitis, and chronic conditions where standard tests are inconclusive.
How to Prepare
- Ensure sample is collected in a sterile container
- For blood, use EDTA or DNA-free tubes as provided
- For tissue or swab, follow the collection kit instructions
- Label the sample with patient ID and date
- Transport at 2-8°C if delayed
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"Shotgun metagenome sequencing is a powerful tool for identifying elusive pathogens, especially in immunocompromised patients or chronic infections where conventional tests fail. This analysis provides a comprehensive view of the microbial community, enabling targeted therapy."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample with visible contamination or hemolysis
- Insufficient DNA quantity or quality
- Improper labeling or missing requisition form
- Sample received after prolonged storage at room temperature
Understanding Your Results
Likely causative agent; consider targeted therapy
May indicate polymicrobial infection or contamination; clinical correlation needed
Could be due to low microbial load, prior antibiotics, or non-infectious etiology
Potential resistance to certain antibiotics; guide antimicrobial selection
Consult your physician if you have persistent symptoms despite treatment, or if you have a weakened immune system and suspect an infection. The results of this test should be discussed with your doctor to plan appropriate management.
Limitations
- ⚠May not detect RNA viruses (requires separate RNA sequencing)
- ⚠Cannot differentiate between live and dead organisms
- ⚠Bioinformatics analysis may miss novel pathogens
- ⚠Requires high-quality DNA; degraded samples may fail
- ⚠Results may be influenced by background contamination
Risks & Considerations
- ●Minimal risk of bruising or infection at blood draw site
- ●No significant risks associated with the test
Interfering Factors
- ●Contamination during sample collection or processing
- ●Low microbial load in sample
- ●Host DNA dominance reducing sensitivity
- ●Antibiotic therapy prior to sample collection
- ●Improper storage or transport of sample
Compare With Similar Tests
| Test | Shotgun Metagenome Sequencing Data Analysis | 16S rRNA Gene Sequencing | Targeted PCR | Culture |
|---|---|---|---|---|
| Comparison | Shotgun Metagenome Sequencing Data Analysis |
Frequently Asked Questions
What is shotgun metagenome sequencing?
How is this test different from 16S rRNA sequencing?
What sample types are accepted?
Do I need to fast before the test?
How long does it take to get results?
Is home sample collection available?
Can this test detect antibiotic resistance?
What is the cost of the test?
Will this test work if I am already on antibiotics?
Are there any risks associated with the test?
How should I interpret the results?
Is the test NABL accredited?
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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
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