Vibrio Cholerae Multiplex Detection & Differentiation of 01, Non 01, 0139 & Ctx Gene (RNA Detection) Qualitative Test
Short Name: Vibrio Cholerae Multiplex PCR
Also known as: Vibrio cholerae PCR, Cholera PCR, Vibrio cholerae RNA detection
Vibrio Cholerae Multiplex Detection & Differentiation of 01, Non 01, 0139 & Ctx Gene (RNA Detection) Qualitative Test test available at DNA Labs India for ₹9,520. Uses Real Time PCR (RT-PCR) on Stool, Water Sample, Culture samples. Results in Reports are typically available within 3 working days after sample collection. Email reports are sent within 36 hours after the report is generated, and phone consultation is available within 24 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of this test is to confirm the diagnosis of cholera by detecting Vibrio cholerae and identifying its serogroup and toxigenic potential. It aids in differentiating between epidemic-causing strains (O1 and O139) and non-epidemic strains (non-O1/non-O139), which may have different clinical implications. Detection of the ctx gene indicates the strain's ability to produce cholera toxin, which is essential for causing severe disease. This information helps clinicians initiate appropriate antibiotic therapy, fluid replacement, and infection control measures promptly. It also supports epidemiological surveillance and outbreak management.
- Test Code
- 6252
- CPT Code
- 87550
- ICD Code
- A00.9
- Price
- ₹9,520
- Sample Type
- Stool, Water Sample, Culture
- Result Time
- Reports are typically available within 3 working days after sample collection. Email reports are sent within 36 hours after the report is generated, and phone consultation is available within 24 hours.
- Fasting Required
- No
- Method
- Real Time PCR (RT-PCR)
Sample Collection
No special preparation is required. However, it is important to inform your physician about any recent antibiotic use. For stool sample, collect in a sterile container. For water sample, use a sterile bottle. For culture, follow laboratory instructions.
Method: Stool: collect in sterile container; Water: collect in sterile bottle; Culture: as per lab instructions
Laboratory Analysis
For stool: collect a small amount (5-10 mL) directly into the sterile container, avoiding contamination with urine or toilet water. For water: collect mid-stream water in a sterile bottle. For culture: as per laboratory guidance.
Report Delivery
Ensure the container is tightly sealed and labeled with your name and date. Transport the sample to the laboratory as soon as possible. If there is a delay, refrigerate the sample (2-8°C) for up to 24 hours.
Timeline: Reports are typically available within 3 working days after sample collection. Email reports are sent within 36 hours after the report is generated, and phone consultation is available within 24 hours.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this test is to confirm the diagnosis of cholera by detecting Vibrio cholerae and identifying its serogroup and toxigenic potential. It aids in differentiating between epidemic-causing strains (O1 and O139) and non-epidemic strains (non-O1/non-O139), which may have different clinical implications. Detection of the ctx gene indicates the strain's ability to produce cholera toxin, which is essential for causing severe disease. This information helps clinicians initiate appropriate antibiotic therapy, fluid replacement, and infection control measures promptly. It also supports epidemiological surveillance and outbreak management.
How to Prepare
- Use sterile, leak-proof containers
- Label the container with patient name, date, and time of collection
- Transport sample to lab within 2 hours if possible
- If delay, store at 2-8°C for up to 24 hours
- Do not freeze stool or water samples
- For culture, follow specific instructions provided by lab
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Cholera is a medical emergency. Rapid and accurate identification of Vibrio cholerae and its toxigenic potential is crucial for appropriate isolation, treatment, and public health control. This multiplex RT-PCR test provides comprehensive information in a single run."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample in inappropriate container
- Sample leaked or contaminated
- Sample not labeled correctly
- Sample received after prolonged delay without proper storage
- Insufficient quantity
Understanding Your Results
Indicates infection with toxigenic O1 strain, the most common cause of epidemic cholera. Immediate treatment and public health notification required.
Indicates infection with toxigenic O139 strain, also capable of causing epidemics. Similar management as O1.
Rare but possible; may cause sporadic cases of cholera-like illness. Clinical correlation advised.
Presence of Vibrio cholerae but without cholera toxin gene. May cause mild gastroenteritis; monitor symptoms.
No Vibrio cholerae detected in the sample. Cholera is unlikely, but other pathogens should be considered.
Consult a physician immediately if you experience severe watery diarrhea, vomiting, or signs of dehydration such as dry mouth, decreased urination, or dizziness. Early treatment is essential to prevent complications.
Limitations
- ⚠This test is qualitative and does not provide bacterial load or quantification
- ⚠Detection of ctx gene does not confirm toxin production; additional testing may be required
- ⚠Non-O1/non-O139 strains may be less pathogenic but can still cause disease
- ⚠Negative result does not rule out cholera if sample was collected after antibiotic initiation
- ⚠Test should be performed by trained personnel in a molecular laboratory
Risks & Considerations
- ●No significant risks associated with sample collection
- ●Minor discomfort during stool collection is unlikely
- ●No radiation or contrast exposure
Interfering Factors
- ●Improper sample collection or transport
- ●Delay in processing leading to bacterial overgrowth or degradation
- ●Inhibition of PCR by substances in stool (e.g., bile salts, bilirubin)
- ●Antibiotic use before sample collection may reduce bacterial load
- ●Cross-contamination during collection or handling
Compare With Similar Tests
| Test | Vibrio Cholerae Multiplex Detection & Differentiation of 01, Non 01, 0139 & Ctx Gene (RNA Detection) Qualitative Test | Stool Culture | Rapid Antigen Test | Microscopy |
|---|---|---|---|---|
| Comparison | Vibrio Cholerae Multiplex Detection & Differentiation of 01, Non 01, 0139 & Ctx Gene (RNA Detection) Qualitative Test |
Frequently Asked Questions
What is the cost of the Vibrio Cholerae Multiplex PCR test?
What sample is required for this test?
How long does it take to get results?
Is fasting required before the test?
What does the test detect?
Can this test be done at home?
What is the turnaround time for home collection?
Is this test covered by insurance?
What is the significance of the ctx gene?
Can this test differentiate between epidemic and non-epidemic strains?
What should I do if my result is positive?
Are there any risks associated with the test?
Related Tests
Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
Book Your Test
Enter your details and we'll connect you within 15 minutes.