Skip to main content
DNA Labs India

17-Hydroxyprogesterone (17-OHP) Stimulation by ACTH Test

DNA Labs India | ISO 9001:2015 Certified

17-Hydroxyprogesterone (17-OHP) Stimulation by ACTH Test

Short Name: 17-OHP Stimulation Test

Also known as: ACTH Stimulation Test for 17-OHP, 17-OHP ACTH Test, Adrenal Stimulation Test

17-Hydroxyprogesterone (17-OHP) Stimulation by ACTH Test test available at DNA Labs India for ₹3,042. Uses Radioimmunoassay on Serum samples. Results in Reports are available within 1 week.. Free home collection in 300+ cities across India.

ACTH Stimulation TestAll Ages🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Ramarao Paidisetty

Dr Ramarao Paidisetty

Consultant Biochemist · Reg: 21504

Last reviewed: September 7, 2026

Overview

To diagnose disorders of the adrenal gland, such as congenital adrenal hyperplasia (CAH), by assessing the adrenal response to ACTH stimulation.

Test Code
41
Price
₹3,042
Sample Type
Serum
Result Time
Reports are available within 1 week.
Fasting Required
Yes
Method
Radioimmunoassay
Step 1

Sample Collection

Overnight fasting is mandatory. First basal sample will be taken between 8-10 am. For menstruating females, sample should be given in first 10 days of the menstrual cycle.

Method: Venipuncture

Step 2

Laboratory Analysis

A baseline blood sample is drawn, then 250 ug of synthetic ACTH (Synacthen) is administered intravenously, and a second sample is drawn 60 minutes later.

Step 3

Report Delivery

Apply pressure to the puncture site to prevent bleeding. Resume normal activities, but monitor for any adverse reactions to ACTH.

Timeline: Reports are available within 1 week.

Patient Instructions

1
Before the Test:Ensure overnight fasting. Avoid medications that may affect results as advised by your doctor.
2
During the Test:The test involves two blood draws and an IV injection of ACTH, typically lasting about 1 hour.
3
After the Test:You may resume normal activities. Monitor for any side effects from ACTH injection.

About This Test

Who Should Get This Test

To diagnose disorders of the adrenal gland, such as congenital adrenal hyperplasia (CAH), by assessing the adrenal response to ACTH stimulation.

How to Prepare

  • Draw fasting early morning baseline sample between 8-10 am
  • Administer 250 ug of synthetic ACTH (Synacthen) IV
  • Draw additional sample at 60 minutes
  • Ship refrigerated or frozen
  • Both tubes must be clearly marked with time drawn
  • Submit both tubes with one test request form

Doctor's Notes

Reviewed by — MBBS, MD (Biochemistry) · Reg. No. 21504

"This test is crucial for early diagnosis of congenital adrenal hyperplasia, which can prevent serious complications if treated promptly."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL per specimen (0.5 mL minimum)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature: Not recommended
Refrigerator: 4 days
Frozen: 4 weeks
Sample Rejection Criteria:
  • Samples not properly labeled with time drawn
  • Hemolyzed or lipemic samples
  • Insufficient sample volume
  • Samples collected at incorrect time

Understanding Your Results

The 17-OHP stimulation test measures the adrenal gland's ability to produce hormones in response to ACTH. Results are interpreted based on the increase in 17-OHP levels from baseline to 60 minutes post-ACTH.
Baseline 17-OHP levels: Normal range is 0.2-2.5 ng/mL
Post-ACTH 17-OHP levels: In healthy individuals, levels increase moderately
Elevated post-ACTH levels (>10 ng/mL in some contexts) may indicate congenital adrenal hyperplasia
Levels should be correlated with clinical findings and other tests
⚠️ When to Consult a Doctor:

If you experience symptoms such as dehydration, vomiting, early puberty, irregular periods, or infertility, consult an endocrinologist for evaluation.

Limitations

  • ⚠Test results should be interpreted in conjunction with clinical symptoms
  • ⚠May not distinguish between all types of CAH
  • ⚠Requires precise timing and administration of ACTH

Risks & Considerations

  • ●Minor bruising or bleeding at puncture site
  • ●Rare allergic reaction to ACTH
  • ●Lightheadedness or fainting

Interfering Factors

  • ●Medications such as corticosteroids
  • ●Stress or illness
  • ●Menstrual cycle phase in females
  • ●Recent ACTH administration

Compare With Similar Tests

Test17-Hydroxyprogesterone (17-OHP) Stimulation by ACTH TestCortisol Stimulation TestACTH Level Test
Comparison17-Hydroxyprogesterone (17-OHP) Stimulation by ACTH TestMeasures cortisol response to ACTHMeasures baseline ACTH levels

Frequently Asked Questions

What is the 17-OHP Stimulation Test?
It is a blood test that measures 17-Hydroxyprogesterone levels before and after ACTH administration to diagnose adrenal disorders like congenital adrenal hyperplasia.
Why is this test performed?
To evaluate adrenal gland function and diagnose conditions such as congenital adrenal hyperplasia, especially in individuals with symptoms like early puberty or infertility.
How should I prepare for the test?
Overnight fasting is required. The first sample is drawn early morning between 8-10 am. For menstruating females, testing should be done in the first 10 days of the menstrual cycle.
What does the test involve?
It involves two blood draws: one baseline sample and another 60 minutes after intravenous injection of synthetic ACTH (Synacthen).
Is fasting required?
Yes, overnight fasting is mandatory for accurate results.
What are the normal results?
Baseline 17-OHP levels typically range from 0.2 to 2.5 ng/mL. Post-ACTH levels vary, but elevated levels may indicate adrenal issues.
What do elevated levels indicate?
Elevated 17-OHP levels after ACTH stimulation may suggest congenital adrenal hyperplasia or other adrenal enzyme deficiencies.
Are there any risks?
Risks are minimal and may include bruising at the puncture site or rare allergic reactions to ACTH.
How long does the test take?
The test procedure takes about 1 hour, including the time for ACTH administration and the second blood draw.
When will I get the results?
Reports are typically available within 1 week via online portal, email, or WhatsApp.
Can this test diagnose CAH?
Yes, it is a key diagnostic test for congenital adrenal hyperplasia, helping to identify adrenal enzyme deficiencies.
Is home collection available?
Yes, DNA Labs India offers free home sample collection for this test across many cities in India.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

Related Tests

ACTH Test

₹1,732

Aldosterone / Renin Direct Ratio Plasma Test

₹9,500

17-Ketosteroids 24 Hour Urine Test

₹8,500

Aldosterone Plasma Test

₹1,872

Alpha Subunit Test

₹18,000

Corticosterone Test

₹4,000
For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

Book Your Test

Enter your details and we'll connect you within 15 minutes.

🧬

Quick Connect

Enter your mobile number and we’ll connect you with the team.

+91

✅ Connecting you now...

🔒 Your number is used to respond to this request.