ACTH Test
Also known as: Adrenocorticotropic Hormone Test, ACTH Level Test
ACTH Test test available at DNA Labs India for ₹1,732. Uses Chemiluminescent Immunoassay on Plasma from EDTA tube samples. Results in Reports are typically available within 1 week after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
To measure ACTH levels in the blood for diagnosing and monitoring adrenal gland disorders, aiding in conditions like Addison's disease and Cushing's syndrome.
- Test Code
- 57
- Price
- ₹1,732
- Sample Type
- Plasma from EDTA tube
- Result Time
- Reports are typically available within 1 week after sample collection.
- Fasting Required
- No
- Method
- Chemiluminescent Immunoassay
Sample Collection
Sample should be collected between 8 a.m. and 10 a.m. due to diurnal variation in ACTH levels. Inform your doctor about any medications you are taking.
Method: Venipuncture
Laboratory Analysis
A healthcare professional will draw blood from a vein in your arm using a needle. The process is quick and minimally invasive.
Report Delivery
Apply pressure to the puncture site with a cotton ball to prevent bleeding and bruising. You can resume normal activities immediately.
Timeline: Reports are typically available within 1 week after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
To measure ACTH levels in the blood for diagnosing and monitoring adrenal gland disorders, aiding in conditions like Addison's disease and Cushing's syndrome.
How to Prepare
- Collect sample between 8 a.m. and 10 a.m.
- Use a pre-chilled Lavender Top (EDTA) tube
- Mix blood well and separate plasma immediately
- Transfer plasma to a sterile screw-capped vial and freeze
- Ship frozen without thawing
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"ACTH test is crucial for diagnosing adrenal gland dysfunction. If symptoms like fatigue or weight changes persist, timely testing can lead to early management and improved outcomes."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not collected in specified time frame
- Incorrect container or improper mixing
- Thawed or improperly stored sample
Understanding Your Results
May indicate primary adrenal insufficiency (Addison's disease), ectopic ACTH syndrome, or stress response.
Level: High ACTH
May indicate Cushing's syndrome, secondary adrenal insufficiency, or pituitary dysfunction.
Level: Low ACTH
Consult an endocrinologist if you have symptoms like unexplained fatigue, weight changes, blood pressure issues, or if test results are abnormal for further evaluation and management.
Limitations
- ⚠Single test may not be conclusive; often requires correlation with other tests
- ⚠Results can be influenced by various physiological factors
- ⚠Does not specify the cause of abnormal levels without additional diagnostics
Risks & Considerations
- ●Minor bruising or pain at the puncture site
- ●Rare risk of infection or excessive bleeding
- ●Fainting or dizziness in some individuals
Interfering Factors
- ●Stress
- ●Medications such as corticosteroids
- ●Time of day due to diurnal variation
- ●Recent surgery or illness
Compare With Similar Tests
| Test | ACTH Test | Cortisol Test | DHEA-S Test |
|---|---|---|---|
| Comparison | ACTH Test | Directly measures cortisol levels; often performed alongside ACTH test to evaluate adrenal function. | Measures dehydroepiandrosterone sulfate, another adrenal hormone, for assessing adrenal disorders. |
Frequently Asked Questions
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