Anti DNase B (Antideoxyribonuclease B) Test
Short Name: Anti DNase B
Also known as: Anti-DNase B Antibody Test, Antideoxyribonuclease B Titer, Anti-DNase B Titer, ADNase B Test, Streptococcal DNase B Antibody Test
Anti DNase B (Antideoxyribonuclease B) Test test available at DNA Labs India for ₹1,404. Uses Latex Enhanced Nephelometry on Serum samples. Results in Reports are typically available on the same day (samples collected on Tuesday or Friday by 9 AM).. Free home collection in 300+ cities across India.
Overview
The primary purpose of the Anti DNase B test is to detect antibodies against the DNase B enzyme produced by Group A Streptococcus. This test helps clinicians confirm evidence of a recent streptococcal infection, which is essential in diagnosing and managing post-streptococcal conditions including acute rheumatic fever, rheumatic heart disease, post-streptococcal glomerulonephritis, and post-streptococcal reactive arthritis. It is also used to monitor the course of a streptococcal infection and to assess the risk of developing complications. When used alongside the ASO titer, it provides a more comprehensive assessment of the immune response to streptococcal antigens.
- Test Code
- 153
- CPT Code
- 86215
- ICD Code
- B95.0
- Price
- ₹1,404
- Sample Type
- Serum
- Result Time
- Reports are typically available on the same day (samples collected on Tuesday or Friday by 9 AM).
- Fasting Required
- No
- Method
- Latex Enhanced Nephelometry
Sample Collection
No special preparation is required. Inform your physician about any medications, especially antibiotics or immunosuppressive drugs, as they may affect the test results. Fasting is not required for this test.
Method: Venipuncture
Laboratory Analysis
A trained phlebotomist will collect approximately 2 mL of blood from a vein in your arm using standard venipuncture technique. The blood is drawn into a Serum Separator Tube (SST). The procedure typically takes less than 5 minutes and causes minimal discomfort.
Report Delivery
Apply gentle pressure to the puncture site with cotton or gauze for a few minutes to stop any bleeding. A small bruise may appear at the site, which is normal and resolves within a few days. You may resume normal activities immediately after sample collection.
Timeline: Reports are typically available on the same day (samples collected on Tuesday or Friday by 9 AM).
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the Anti DNase B test is to detect antibodies against the DNase B enzyme produced by Group A Streptococcus. This test helps clinicians confirm evidence of a recent streptococcal infection, which is essential in diagnosing and managing post-streptococcal conditions including acute rheumatic fever, rheumatic heart disease, post-streptococcal glomerulonephritis, and post-streptococcal reactive arthritis. It is also used to monitor the course of a streptococcal infection and to assess the risk of developing complications. When used alongside the ASO titer, it provides a more comprehensive assessment of the immune response to streptococcal antigens.
How to Prepare
- No fasting required before the test
- Stay well hydrated before sample collection
- Inform the phlebotomist of any bleeding disorders or if you are on blood-thinning medications
- Wear clothing with sleeves that can be easily rolled up
- Bring your doctor's prescription or test requisition form
- Avoid strenuous exercise immediately before sample collection
Doctor's Notes
"The Anti DNase B test is a valuable serological marker for detecting evidence of prior Group A Streptococcal (GAS) infection. It is particularly useful when performed alongside the Anti-Streptolysin O (ASO) titer, as the combination increases diagnostic sensitivity. Elevated Anti DNase B levels may persist for weeks to months after a streptococcal infection and are clinically relevant in evaluating patients with suspected rheumatic fever, post-streptococcal glomerulonephritis, or post-streptococcal reactive arthritis. I recommend this test when a patient presents with symptoms suggestive of a post-streptococcal complication, especially if ASO titers are inconclusive. Early identification of a recent streptococcal infection can guide timely antibiotic therapy and help prevent long-term cardiac or renal complications."
Test Parameters & Specifications
Sample Stability
- Hemolyzed samples
- Lipemic samples
- Samples collected in incorrect tube type (not SST)
- Samples older than 6 hours at room temperature without refrigeration
- Insufficient sample volume (less than 1 mL serum)
- Samples without proper labeling or identification
Understanding Your Results
Elevated Anti DNase B titer
Suggests a recent or past Group A Streptococcal infection. Elevated levels may persist for several months. Clinical correlation is essential. Consider paired testing (acute and convalescent) to confirm recent infection.
Clinical action: Correlate with clinical symptoms (fever, sore throat, skin lesions), ASO titer, complete blood count, ESR, CRP, and urinalysis. Refer to a specialist for evaluation of post-streptococcal complications if indicated.
Normal Anti DNase B titer
Does not definitively exclude a recent streptococcal infection. Some patients, particularly those with skin infections or early testing, may have normal levels. Antibody rise may take 1–2 weeks after infection.
Clinical action: If clinical suspicion remains high, repeat testing in 2–4 weeks. Consider additional tests such as throat culture, rapid strep test, or ASO titer.
Borderline Anti DNase B titer
May indicate a resolving infection, early infection, or individual variability in immune response. Requires correlation with clinical findings and other laboratory markers.
Clinical action: Repeat testing in 2–4 weeks to assess for a rising titer. Monitor clinical symptoms and obtain complementary investigations.
Consult your doctor if you have had a recent sore throat or skin infection followed by joint pain, swelling, skin rashes, chest pain, shortness of breath, unexplained fever, blood in urine, facial or limb swelling, or prolonged fatigue. These symptoms may indicate post-streptococcal complications such as rheumatic fever or glomerulonephritis that require prompt medical evaluation and treatment. Children who have had recurrent strep throat infections should also be evaluated.
Limitations
- ⚠A single elevated titer does not distinguish between a current and a past infection without a baseline comparison
- ⚠The test detects antibodies and does not identify the bacteria directly; culture or rapid antigen tests are needed for direct detection
- ⚠Antibody levels may vary based on geographic region, age, and prior exposure history
- ⚠A normal result does not completely rule out a recent streptococcal infection, as some individuals mount a weak antibody response
- ⚠Results should always be interpreted in conjunction with clinical findings and other laboratory tests such as ASO titer
Risks & Considerations
- ●Minor bruising or soreness at the needle insertion site
- ●Slight risk of infection at the puncture site (very rare)
- ●Fainting or lightheadedness during blood draw (uncommon)
- ●No significant long-term risks associated with this test
Interfering Factors
- ●Recent antibiotic treatment may suppress the antibody response and yield falsely low results
- ●Very early testing (within 1 week of infection onset) may not yet show elevated antibody levels
- ●Immunosuppressive medications or conditions may blunt the antibody response
- ●Cross-reactive antibodies from other bacterial infections may occasionally influence results
- ●Hemolyzed or lipemic serum samples may affect the accuracy of nephelometric measurements
Compare With Similar Tests
| Test | Anti DNase B (Antideoxyribonuclease B) Test | Anti-Streptolysin O (ASO) Titer |
|---|---|---|
| Comparison | Anti DNase B (Antideoxyribonuclease B) Test |
Frequently Asked Questions
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