Skip to main content
DNA Labs India

CYP3A4*22 Genotyping Test

DNA Labs India | ISO 9001:2015 Certified

CYP3A4*22 Genotyping Test

Short Name: CYP3A4*22

Also known as: CYP3A4 rs35599367 Genotyping Test, CYP3A4*22 Pharmacogenomic Test, CYP3A4 Variant Detection Test, Cytochrome P450 3A4 Genotyping Test

CYP3A4*22 Genotyping Test test available at DNA Labs India for ₹9,000. Uses PCR (Polymerase Chain Reaction), DNA Sequencing on Whole Blood samples. Results in 10 Working Days from sample receipt. Submit sample by 11 am for timely processing.. Free home collection in 300+ cities across India.

PhysicianAll Ages🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SULOCHANA HEMCHANDRA HOLLA

Dr SULOCHANA HEMCHANDRA HOLLA

Consultant Medical Geneticist · Reg: 8532

Last reviewed: September 7, 2026

Overview

The purpose of the CYP3A4*22 Genotyping Test is to identify whether an individual carries the CYP3A4*22 genetic variant that causes reduced activity of the CYP3A4 enzyme. This enzyme is responsible for metabolizing a large proportion of commonly prescribed drugs. By detecting this variant, the test helps physicians personalize medication choices and dosages, thereby reducing the risk of adverse drug reactions, drug toxicity, and therapeutic failure. The test is particularly valuable for patients undergoing treatment with CYP3A4-substrate medications such as statins, immunosuppressants, certain antibiotics, and anticancer agents.

Test Code
467
Price
₹9,000
Sample Type
Whole Blood
Result Time
10 Working Days from sample receipt. Submit sample by 11 am for timely processing.
Fasting Required
No
Method
PCR (Polymerase Chain Reaction), DNA Sequencing
Step 1

Sample Collection

Ensure a duly filled Genomics Clinical Information Requisition Form (Form 20) is completed and submitted with the sample. No fasting is required. Inform the phlebotomist of any recent blood transfusions or current medications.

Method: Venipuncture

Step 2

Laboratory Analysis

A standard venipuncture will be performed to collect 4 mL (minimum 2 mL) of whole blood into a Lavender top (EDTA) tube. The procedure typically takes less than 5 minutes.

Step 3

Report Delivery

Apply pressure to the puncture site for a few minutes to prevent bruising. The sample will be shipped refrigerated to the laboratory. Do not freeze the sample. Results will be available within 10 working days.

Timeline: 10 Working Days from sample receipt. Submit sample by 11 am for timely processing.

Patient Instructions

1
Before the Test:No special preparation such as fasting is required for the CYP3A4*22 Genotyping Test. However, a duly filled Genomics Clinical Information Requisition Form (Form 20) must be completed and submitted along with the blood sample. Inform your physician about any medications you are currently taking and any recent blood transfusions.
2
During the Test:The test involves a standard blood draw (venipuncture). A healthcare professional will collect approximately 4 mL of whole blood from a vein in your arm into a Lavender top (EDTA) tube. The procedure typically takes less than 5 minutes and involves minimal discomfort.
3
After the Test:After blood collection, pressure will be applied to the puncture site with a cotton ball or bandage. You may resume normal activities immediately. The sample will be transported refrigerated to the laboratory for DNA extraction and analysis. Results will be available within 10 working days and can be accessed online, via email, or WhatsApp.

About This Test

Who Should Get This Test

The purpose of the CYP3A4*22 Genotyping Test is to identify whether an individual carries the CYP3A4*22 genetic variant that causes reduced activity of the CYP3A4 enzyme. This enzyme is responsible for metabolizing a large proportion of commonly prescribed drugs. By detecting this variant, the test helps physicians personalize medication choices and dosages, thereby reducing the risk of adverse drug reactions, drug toxicity, and therapeutic failure. The test is particularly valuable for patients undergoing treatment with CYP3A4-substrate medications such as statins, immunosuppressants, certain antibiotics, and anticancer agents.

How to Prepare

  • Duly filled Genomics Clinical Information Requisition Form (Form 20) is mandatory
  • Collect 4 mL (2 mL min.) whole blood in 1 Lavender top (EDTA) tube
  • Ship the sample refrigerated. DO NOT FREEZE
  • Label the sample correctly with patient details and date of collection
  • Submit the sample by 11 am for timely processing

Doctor's Notes

Reviewed by — MBBS, MD (Medical Genetics) · Reg. No. 8532

"Pharmacogenomic testing such as the CYP3A4*22 Genotyping Test is a valuable tool for personalized medicine. In my clinical practice, I have observed that patients who carry the CYP3A4*22 variant may experience unpredictable responses to commonly prescribed medications including statins, immunosuppressants, benzodiazepines, and calcium channel blockers. The CYP3A4 enzyme metabolizes nearly 50% of all marketed drugs, making this variant clinically significant across multiple therapeutic areas. Identifying this variant before initiating therapy allows for proactive dose adjustments, reducing the risk of adverse drug reactions and improving overall treatment efficacy. I recommend this test for any patient with a history of unexplained side effects or poor therapeutic response to CYP3A4-substrate drugs."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeWhole Blood
Sample Volume4 mL (2 mL min.) whole blood in 1 Lavender top (EDTA) tube
ContainerLavender top (EDTA) tube
Collection MethodVenipuncture

Sample Stability

Room Temperature
Refrigerated (2-8°C)
Frozen
Sample Rejection Criteria:
  • Sample received without the mandatory Genomics Clinical Information Requisition Form (Form 20)
  • Hemolyzed or clotted blood sample
  • Sample collected in incorrect tube (non-EDTA)
  • Frozen sample
  • Insufficient sample volume (less than 2 mL)
  • Unlabeled or mislabeled sample

Understanding Your Results

The CYP3A4*22 Genotyping Test results indicate whether you carry the CYP3A4*22 variant allele and predict your metabolizer phenotype. Understanding your metabolizer status helps your doctor determine the most appropriate drug and dosage for your treatment. Below is a general guide to interpreting the results:
📊

No CYP3A4*22 variant detected. CYP3A4 enzyme activity is normal. Standard drug dosages are generally appropriate.

📊

One copy of the CYP3A4*22 variant detected. CYP3A4 enzyme activity is reduced. Dose adjustments or alternative medications may be recommended for certain drugs.

📊

Two copies of the CYP3A4*22 variant detected. CYP3A4 enzyme activity is significantly reduced. Significant dose reductions or alternative medications not metabolized by CYP3A4 are recommended.

⚠️ When to Consult a Doctor:

Consult your doctor if you experience any of the following: unexplained muscle pain or weakness, abdominal pain or discomfort, dark urine, yellowing of the skin or eyes (jaundice), fever or flu-like symptoms while taking medications metabolized by CYP3A4. Additionally, consult your doctor to discuss your CYP3A4*22 test results and to determine the appropriate medication and dosage adjustments based on your metabolizer status. Always consult a healthcare professional before making any changes to your medication regimen.

Limitations

  • ⚠This test only detects the CYP3A4*22 variant and does not identify other CYP3A4 genetic variations
  • ⚠Results reflect genetic predisposition and do not account for environmental factors, drug interactions, or disease states that also affect drug metabolism
  • ⚠Clinical utility depends on the specific medication being used; not all CYP3A4-substrate drugs are equally affected by this variant
  • ⚠Ethnic variation in allele frequency means the clinical significance may differ across populations
  • ⚠This test should be interpreted in conjunction with other clinical findings and is not a standalone diagnostic tool

Risks & Considerations

  • ●Minor bruising or discomfort at the blood draw site
  • ●Rare risk of slight dizziness or lightheadedness during venipuncture
  • ●Very rare risk of infection at the puncture site

Interfering Factors

  • ●Hemolyzed blood samples may affect DNA quality and test accuracy
  • ●Recent blood transfusions within the past 30 days may interfere with genotyping results
  • ●Contamination during sample collection or transport may compromise results
  • ●Improper sample storage (freezing or extended room temperature exposure) may degrade DNA

Compare With Similar Tests

TestCYP3A4*22 Genotyping TestCYP2C19 Genotyping TestCYP2D6 Genotyping TestCYP2C9 Genotyping TestComprehensive Pharmacogenomics Panel
ComparisonCYP3A4*22 Genotyping Test

Frequently Asked Questions

What is the CYP3A4*22 Genotyping Test?
The CYP3A4*22 Genotyping Test is a pharmacogenomic genetic test that detects the CYP3A4*22 variant (rs35599367), a specific mutation in the CYP3A4 gene. This variant reduces the activity of the CYP3A4 enzyme, which is responsible for metabolizing many commonly prescribed medications. Identifying this variant helps your doctor personalize your medication and dosage for safer and more effective treatment.
Why is the CYP3A4*22 Genotyping Test important?
This test is important because it helps identify individuals who may metabolize certain drugs differently due to a genetic variant. People carrying the CYP3A4*22 allele may have reduced enzyme activity, leading to higher drug levels in the blood. This information allows doctors to adjust drug dosages or select alternative medications to minimize the risk of adverse reactions and improve treatment outcomes.
What sample is required for the CYP3A4*22 Genotyping Test?
The test requires 4 mL (minimum 2 mL) of whole blood collected in a Lavender top (EDTA) tube. The sample should be shipped refrigerated and must not be frozen. A duly filled Genomics Clinical Information Requisition Form (Form 20) is mandatory for processing the sample.
How long does it take to get the CYP3A4*22 Genotyping Test results?
The turnaround time for the CYP3A4*22 Genotyping Test is approximately 10 working days from the date of sample receipt at the laboratory. Samples should be submitted by 11 am for timely processing. Reports are accessible through the online portal, email, or WhatsApp.
What does a positive CYP3A4*22 result mean?
A positive result indicates that you carry one or two copies of the CYP3A4*22 variant allele. This means you have reduced CYP3A4 enzyme activity, which may cause certain drugs to be metabolized more slowly than normal, potentially leading to higher drug levels and increased risk of side effects. Your doctor can use this information to adjust your medication regimen appropriately.
Is the CYP3A4*22 Genotyping Test covered by insurance in India?
Coverage for genetic and pharmacogenomic testing varies among insurance providers in India. Some private insurance plans may cover pharmacogenomic tests when prescribed by a physician. Government schemes such as PMJAY, CGHS, ECHS, and ESIC may have limited or no coverage for this test. It is advisable to check directly with your insurance provider before scheduling the test.
Who should consider getting the CYP3A4*22 Genotyping Test?
This test is recommended for individuals who have experienced adverse drug reactions, unexpected side effects, or poor response to medications metabolized by CYP3A4. It is also beneficial for patients starting medications such as statins, immunosuppressants, or certain chemotherapy agents, those on multiple medications, and individuals with a family history of altered drug metabolism.
What is the role of the CYP3A4 enzyme in drug metabolism?
The CYP3A4 enzyme, encoded by the CYP3A4 gene, is a member of the cytochrome P450 family of enzymes. It is the most abundant drug-metabolizing enzyme in the liver and intestine, responsible for metabolizing approximately 50% of all marketed drugs. Variants in the CYP3A4 gene can alter enzyme activity and affect how your body processes medications, leading to variations in drug efficacy and safety.
Which drugs are metabolized by CYP3A4?
CYP3A4 metabolizes a wide range of medications including statins (atorvastatin, simvastatin), immunosuppressants (tacrolimus, cyclosporine), certain antibiotics (erythromycin, clarithromycin), antifungal agents (ketoconazole), benzodiazepines (midazolam, diazepam), calcium channel blockers (amlodipine, nifedipine), and some anticancer drugs. Your doctor will advise which specific medications are relevant to your treatment plan.
How accurate is the CYP3A4*22 Genotyping Test?
The CYP3A4*22 Genotyping Test at DNA Labs India uses advanced PCR and DNA sequencing methods, which provide highly accurate and reliable results for detecting the specific rs35599367 variant. These molecular techniques have very high analytical sensitivity and specificity. However, it is important to note that this test only detects the CYP3A4*22 variant and does not cover all possible CYP3A4 genetic variations.
Do I need to fast before the CYP3A4*22 Genotyping Test?
No, fasting is not required for the CYP3A4*22 Genotyping Test. Since this is a genetic test that analyzes your DNA from a blood sample, food intake does not affect the results. However, a duly filled Genomics Clinical Information Requisition Form (Form 20) must be submitted along with the sample for the test to be processed.
Can the CYP3A4*22 Genotyping Test be done at home?
Yes, DNA Labs India offers free home sample collection for the CYP3A4*22 Genotyping Test when booked online. Our trained phlebotomists will visit your home at a convenient time to collect the blood sample. This service is available across numerous cities in India including Mumbai, Delhi, Bangalore, Hyderabad, Chennai, Kolkata, Pune, Ahmedabad, and many more. You can book your appointment through our website or by calling our helpline number.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

Related Tests

Clopidogrel CYP2C19 Genotype Test

₹50,000

Tacrolimus Genotyping (CYP3A5) Test

₹7,000

ADRB2 Genotyping for Beta-2 Agonist Responsiveness Test

₹5,500

COMT Genotyping Test

₹42,120

HLA Hypersensitivity to Allopurinol (B*58:01) Test

₹8,000

HLA Hypersensitivity to Carbamazepine & Phenytoin (B*15:02) Test

₹8,000
For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

Book Your Test

Enter your details and we'll connect you within 15 minutes.

🧬

Quick Connect

Enter your mobile number and we’ll connect you with the team.

+91

✅ Connecting you now...

🔒 Your number is used to respond to this request.