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Epstein-Barr Virus Early RNA (EBER-ISH) In-Situ-Hybridization Test

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Epstein-Barr Virus Early RNA (EBER-ISH) In-Situ-Hybridization Test

Short Name: EBER-ISH Test

Also known as: EBER In-Situ Hybridization Test, EBV Early RNA ISH Test

Epstein-Barr Virus Early RNA (EBER-ISH) In-Situ-Hybridization Test test available at DNA Labs India for ₹6,000. Uses In-Situ-Hybridization on Tumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded (FFPE) block samples. Results in 10-12 business days. Free home collection in 300+ cities across India.

Diagnostic🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

To detect the presence of Epstein-Barr Virus (EBV) in tissue samples, particularly in cases of suspected EBV-associated cancers such as lymphomas and nasopharyngeal carcinoma, aiding in accurate diagnosis and treatment planning.

Test Code
15
Price
₹6,000
Sample Type
Tumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded (FFPE) block
Result Time
10-12 business days
Fasting Required
No
Method
In-Situ-Hybridization
Step 1

Sample Collection

Ensure the tissue sample is properly fixed and labeled with patient details and biopsy site.

Method: Biopsy collection by healthcare professional

Step 2

Laboratory Analysis

Biopsy performed by a trained healthcare professional under sterile conditions to obtain adequate tissue.

Step 3

Report Delivery

Store the sample at room temperature and transport it promptly to the laboratory to preserve integrity.

Timeline: 10-12 business days

Patient Instructions

1
Before the Test:No special preparation is required for the test itself, but ensure the biopsy procedure is scheduled and clinical history is documented.
2
During the Test:The tissue sample is processed in the laboratory, where probes hybridize to EBER RNA, and results are visualized microscopically.
3
After the Test:Monitor the biopsy site for any signs of infection or bleeding; results will be available online or via delivery in 10-12 days.

About This Test

Who Should Get This Test

To detect the presence of Epstein-Barr Virus (EBV) in tissue samples, particularly in cases of suspected EBV-associated cancers such as lymphomas and nasopharyngeal carcinoma, aiding in accurate diagnosis and treatment planning.

How to Prepare

  • Submit tumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded (FFPE) block
  • Ship at room temperature
  • Include a copy of the histopathology report, site of biopsy, and clinical history

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This test is crucial for confirming EBV involvement in certain cancers, aiding in targeted therapy decisions."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeTumor tissue in 10% Formal-saline or Formalin fixed paraffin embedded (FFPE) block
Sample VolumeNot specified; depends on biopsy size
ContainerFormal-saline container or FFPE block
Collection MethodBiopsy collection by healthcare professional

Sample Stability

Room temperature: Stable for several days if properly fixed
Refrigerator: Not recommended for this sample type
Frozen: Not applicable
Sample Rejection Criteria:
  • Inadequate fixation or tissue integrity
  • Missing clinical information or histopathology report
  • Contaminated or degraded sample

Understanding Your Results

Results are interpreted based on the detection of EBV Early RNA in tissue cells, indicating viral presence or absence.
Positive: EBV RNA detected, confirming EBV infection in the tissue; often associated with certain cancers
Negative: No EBV RNA detected; but clinical symptoms may warrant further testing
Inconclusive: Due to technical issues or insufficient sample; retest may be required
⚠️ When to Consult a Doctor:

If experiencing persistent symptoms like fever, sore throat, or swollen lymph nodes, or if diagnosed with EBV-associated cancer, consult a doctor for test recommendation and follow-up.

Limitations

  • ⚠Only detects EBV RNA, not DNA or antibodies
  • ⚠Requires specialized laboratory equipment and expertise
  • ⚠False negatives possible with low viral load or improper sample handling

Risks & Considerations

  • ●Minimal risk associated with biopsy procedure, such as infection or bleeding at the site
  • ●Rare allergic reactions to local anesthesia if used

Interfering Factors

  • ●Inadequate tissue fixation
  • ●Sample degradation during transport
  • ●Technical errors in hybridization process

Compare With Similar Tests

TestEpstein-Barr Virus Early RNA (EBER-ISH) In-Situ-Hybridization TestEBV Antibody TestEBV DNA PCR
ComparisonEpstein-Barr Virus Early RNA (EBER-ISH) In-Situ-Hybridization Test

Frequently Asked Questions

What is the EBER-ISH test?
The EBER-ISH test is a diagnostic method that uses in-situ hybridization to detect Epstein-Barr Virus RNA in tissue samples, helping identify EBV in cancers.
Why is the EBER-ISH test performed?
It is performed to confirm the presence of EBV in suspected cancers like lymphomas, aiding in diagnosis, prognosis, and treatment planning.
How much does the EBER-ISH test cost in India?
The test costs INR 6000 at DNA Labs India, with discounts available for online bookings and home collection.
What sample is required for the EBER-ISH test?
A tumor tissue sample fixed in 10% Formal-saline or as a Formalin fixed paraffin embedded (FFPE) block is required, along with a histopathology report.
Is home collection available for the EBER-ISH test?
Yes, DNA Labs India offers free home sample collection across India for this test when booked online.
How long does it take to get EBER-ISH test results?
Results are typically available within 10-12 business days, delivered via online portal, email, or WhatsApp.
What are the symptoms of Epstein-Barr virus infection?
Symptoms may include fever, sore throat, fatigue, swollen lymph nodes, enlarged spleen, loss of appetite, headache, and skin rash, though many infections are asymptomatic.
How accurate is the EBER-ISH test?
The test is highly accurate for detecting EBV RNA in tissue cells, offering better specificity than blood tests for localized infections.
Are there any risks associated with the EBER-ISH test?
Risks are minimal and primarily related to the biopsy procedure, such as infection or bleeding at the collection site.
What if the EBER-ISH test result is positive?
A positive result indicates EBV presence in the tissue; consult your doctor for further evaluation and potential treatment options.
Can the EBER-ISH test detect EBV in blood?
No, this test is designed for tissue samples only. Blood tests like EBV antibody or PCR tests are used for blood detection.
How should I prepare for the EBER-ISH test?
No special preparation is needed, but ensure your biopsy is scheduled and all relevant clinical information is provided to the lab.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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