Immunohistochemistry C4d Test
Short Name: C4d Test
Also known as: C4d Immunohistochemistry, Complement C4d Test
Immunohistochemistry C4d Test test available at DNA Labs India for ₹3,500. Uses Immunohistochemistry on Tissue sample in 10% Formal-saline or Formalin fixed paraffin embedded block samples. Results in Report available within 5-7 days from sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
To detect C4d protein in tissue samples for diagnosing antibody-mediated rejection in kidney transplants and autoimmune disorders like SLE, rheumatoid arthritis, and glomerulonephritis.
- Test Code
- 966
- Price
- ₹3,500
- Sample Type
- Tissue sample in 10% Formal-saline or Formalin fixed paraffin embedded block
- Result Time
- Report available within 5-7 days from sample receipt.
- Fasting Required
- No
- Method
- Immunohistochemistry
Sample Collection
Ensure proper biopsy sample with clinical history and histopathology report provided.
Method: Biopsy or surgical procedure
Laboratory Analysis
Tissue sample collected via biopsy or surgery under sterile conditions.
Report Delivery
Sample preserved in formalin and shipped to laboratory at room temperature.
Timeline: Report available within 5-7 days from sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
To detect C4d protein in tissue samples for diagnosing antibody-mediated rejection in kidney transplants and autoimmune disorders like SLE, rheumatoid arthritis, and glomerulonephritis.
How to Prepare
- Submit tissue in 10% Formal-saline or Formalin fixed paraffin embedded block
- Ship at room temperature
- Provide a copy of the Histopathology report, Site of biopsy, and Clinical history
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"This test is crucial for evaluating antibody-mediated rejection in kidney transplants and diagnosing autoimmune kidney diseases, aiding in timely clinical management."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Improperly fixed samples
- Insufficient tissue quantity
- Missing clinical documentation
Understanding Your Results
Positive C4d staining
Indicates complement activation, often seen in antibody-mediated rejection or autoimmune disorders like SLE.
Action: Consult a nephrologist or immunologist for further evaluation and management.
Negative C4d staining
No significant complement activation detected in the tissue sample.
Action: Correlate with clinical symptoms and other diagnostic tests for comprehensive assessment.
If symptoms of autoimmune disorders persist, after kidney transplant evaluation, or if C4d test results are abnormal.
Limitations
- ⚠May not be specific for a single disease; requires clinical correlation
- ⚠Results depend on proper sample collection and handling
Risks & Considerations
- ●Risks associated with biopsy procedure, such as bleeding or infection
- ●Minimal risk from the staining process itself
Interfering Factors
- ●Improper sample fixation
- ●Technical errors in staining procedure
- ●Insufficient tissue sample
Compare With Similar Tests
| Test | Immunohistochemistry C4d Test | Antinuclear Antibody (ANA) Test | Complement C3 Test | Renal Biopsy Panel |
|---|---|---|---|---|
| Comparison | Immunohistochemistry C4d Test | Often used alongside C4d test for autoimmune diagnosis to detect nuclear antibodies. | Measures complement C3 levels in blood; C4d test focuses on tissue-specific complement activation. | C4d staining is part of a comprehensive renal biopsy evaluation for kidney disorders. |
Frequently Asked Questions
What is the Immunohistochemistry C4d Test?
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